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Articles by Raoudha Abdellaoui
Total Records ( 2 ) for Raoudha Abdellaoui
  Raoudha Abdellaoui , Hatem Cheik M’Hamed , M`barek Ben Naceur , Leila Bettaieb-Kaab and Jeannette Ben Hamida
  This research aim is to study whether we still have genetic diversity of barley all around the country, or there has been genetic erosion leading to a reduction in landraces barley cultivars. To fulfill this purpose, some ecotypes were collected from few frequented various bioclimatic regions and morpho-physiological and molecular level were studied. Our results showed differences among the ecotypes studied based on the morpho-physiological criteria such as heading date, density and ear length and response to saline stress. The molecular analysis showed the limits of the morpho-physiological approach. In fact, identical ecotypes were found grown in different parts of the country and the morpho-physiological differences observed could be due to environmental conditions’ adaptation acquired over time. Also, ecotypes that were grown mixed together in the same area and having similar physiological behavior were found different using the RAPD markers method. Important local barley genetic variability was found, concluding the Tunisian germplasm richness.
  Raoudha Abdellaoui , Faouzia Yahyaoui and Mohamed Neffati
  Retama raetam is a stem-assimilating, C3, evergreen, medicinal plant species, desert legume common to arid ecosystems around the Mediterranean basin. This study addresses the genetic diversity and relationship among and within three populations collected from different habitats in southern Tunisia by Random Amplified Polymorphic DNA (RAPD). Estimates of the percentage of polymorphic bands, Shannon’s diversity information index and Nei’s gene diversity index were determined. Results showed that population from the Island Djerba has the lowest Nei’s gene diversity; this also was for Shannon diversity index. An analysis of molecular variance indicated that the majority of variation existed within populations (68%) and that there was significant differentiation among populations (ΦPT = 0.316, p<0.001). Genetic distance (ΦPT based values) between pair-wise populations ranged from 0.098 to 0.505 and the differentiation between pair-wise populations was significant when individual pairs of populations were compared. Based on the coefficient of gene differentiation (Gst), gene flow (Nm) was estimated and was found to vary from 0.490 to 4.609 between pair-wise populations and 1.42 among populations. The results of UPGMA cluster analysis and PCoA analysis indicated that most variation occurred within populations and that genetic differentiation had happened between populations. These findings are important for a better understanding of the adaptive strategy of R. raetam in southern Tunisia and will be useful for conservation managers to work out an effective strategy to protect this important species.
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