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Articles by Ranjan Kumar Bhagobaty
Total Records ( 2 ) for Ranjan Kumar Bhagobaty
  Santa Ram Joshi , Ranjan Kumar Bhagobaty and Rakshak Kumar
  Impact of roadside pollution on the population of leaf surface microorganisms of Alnus nepalensis D. Don and Pinus kesiya Royle Ex Gordon was studied at a hilly terrain in Eastern Himalayas. Changes in the population of microbes especially fungi and bacteria due to roadside pollution was noted. Leaves were collected at sites 8 m and 1 km away from the National Highway No. 44 and were analysed for microbial population, heavy metals and sulphur accumulation. Leaves of both tree species closer to the road contained higher amounts of heavy metals than those at 1 km site. The population of bacteria and most fungi species was higher at 1 km site than the site closer to the highway. Diversity of micro fungal community in phylloplane at the two sites differed significantly. Counts of fungal units (cfu) and bacteria propagules showed significant negative correlation with the concentrations of metals and sulphur. Some fungal forms including Fusarium oxysporum, Mortierella sp. and Aureobasidium pollulans were abundant in the polluted roadside compared to other species of fungi.
  Ranjan Kumar Bhagobaty and Abdul Malik
  In the present study wastewater irrigated agricultural soil with a previous history of chlorpyrifos use was examined for its capacity to harbor bacteria capable of utilizing it as a sole source of carbon. Four bacterial isolates designated as RA-3, RA-5, RA-10, RA-20, isolated from the soil, using enrichment culture technique showed promising capability to utilize chlorpyrifos as a carbon source for their growth. Morphological and biochemical tests performed on the bacteria indicated that they might belong to the genus Pseudomonas. Thin layer chromatography and tetrazolium reduction assay showed that the strains were capable of degrading chlorpyrifos. All the chlorpyrifos degrading bacterial isolates were also tested for their antibiotic sensitivity against 10 antibiotics/drugs. All the isolates were sensitive to gentamycin and methicillin. RA-10 and RA-3 were sensitive to ampicillin whereas RA-5 was resistant and RA-20 showed intermediate range of sensitivity. RA-5 and RA-3 were sensitive to chloramphenicol whereas, RA-10 and RA-20 showed intermediate sensitivity. RA-5 and RA-20 showed resistance against co-trimoxazole and nalidixic acid. RA-5 and RA-10 showed intermediate sensitivity to tetracycline whereas RA-20 was resistant and RA-3 sensitive to it. All the bacterial isolates were also found to harbor a single plasmid. This leads us to believe that the soils with previous exposure to chlorpyrifos contain a diverse range of bacteria having novel organophosphorus hydrolase enzyme systems for causing the enhanced biodegradation of this toxic pesticide in the environment. Further elucidation of the enzymatic and molecular mechanisms involved in the process will help in creating possible bioremediation technologies using the soil bacteria.
 
 
 
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