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Articles by Ranajit Kumar Shaha
Total Records ( 4 ) for Ranajit Kumar Shaha
  N.K. Sana , I. Hossin , E.M. Haque and Ranajit Kumar Shaha
  Ionic effect on the degradation of oil seeds (Brassica napus L) storage lipid was investigated during germination. Lipid degradating enzyme lipase was identified in germinating Brassica napus L and the enzyme was purified to homogeneity by extraction, Sephadex G-50, DEAE and CM-cellulose column chromatography. The enzyme was purified to 67.59 fold with the final specific activity of 39.10 mmol min-1 mg-1 at 37°C using triolein as a substrate. The purified enzyme showed a single band when it was subjected to SDS / PAGE electrophoresis. The SDS/PAGE electrophoresis indicated a molecular mass of 34 kDa for this lipase which corresponding to the high-resolution gel filtration. The optimum pH and temperature for hydrolysis of Oleic acid were 7.0 and 37°C, respectively. In the presence of Ca 2+ and Bi 3+, the lipase activity was dramatically enhanced by 165 and 124%, respectively. Fe3+, Fe2+, Zn2+, Hg2+ and Cu2+ could inhibit this lipase but Al 3+, Pb 2+ showed no influence on hydrolysis activity. Km of this enzyme was 0.23 mM.
  Dipak Kumar Paul and Ranajit Kumar Shaha
  Seventeen different common citrus fruits have been analyzed for their content of nutrients: carbohydrate, protein, lipids, vitamins,carotene, thiamine, riboflavin, ascorbic acid, important macro and micro minerals such as sodium, potassium, calcium, magnesium, phosphorous, iron, zinc and copper. Carbohydrate, protein and fats in citrus fruits varied from 4.60-8.50, 5.80-7.90 and 2.50-9.50 g, respectively. The content of carotene, thiamine, riboflavin and ascorbic acid varied different amounts in citrus fruits. The highest contents of Carotene fruits are orange and tomato. Among the analyzed citrus fruits pineapple content the maximum amount of thiamine (0.20 mg 100-1 g) and wood apple content maximum riboflavin (0.15 mg 100-1 g). Amla (Indian gooseberry) fruits contents the highest ascorbic acid 600 mg 100-1 g of fresh edible parts of fruits. Lemon contained the highest amount of calcium. The highest amount of magnesium was found in Black berry (49.80 mg 100-1 g of edible portion of the fruits). Sodium present in different citrus fruits ranged from 1.0 to 28 mg 100-1 g. Wood apple contained the highest amount of phosphorus (98.90 mg 100-1 g) among all citrus fruits. Tomato contained the highest amount of Potassium (275 mg 100-1 g). The iron content in different fruits ranged from 0.10 to 38 mg 100-1 g. Zinc present in fruits ranged between 0.18 to 0.48 mg 100-1 g. Copper content in different fruits analyzed ranged from 0.1 to 0.68 mg 100-1 g of fruits.
  Dipak Kumar Paul and Ranajit Kumar Shaha
  The present study was to investigate the nutritive values such as moisture, ash, fibre, protein, fat, carbohydrate as well as Phytate and zinc content of different locally developed improved eleven varieties of wheat, four varieties of legumes. The nutritive values, Phytate and zinc content were analyzed by standard methods. Among all cereals and legumes sample studied, fat content varies from 1.9-3.12 g; protein content varies from 8.9-12.5 g and carbohydrate content varies from 58-71.3 g, respectively. From the study it is revealed Akbar variety of wheat content the highest amount of fat (3.12 g) and carbohydrate (72.5 g) and grass pea contain the highest amount of protein (28.2 g/100 g edible portion). Phytate and zinc contents of different varieties of wheat, legumes are studied. Among the tested samples grass pea was the highest Phytate content (745.5 mg/100 g edible portion). Zinc content was found in different wheat varieties (1.08-3.94 mg) and legumes (2.75-3.96 mg). Maximum content of zinc in legumes (grass peas, 3.96 mg) and wheat (kanchan and Gaurav varieties 3.94 mg). Maximum Phytate and zinc contents of whole-wheat flour were found 712 mg (Akbar variety) and 3.94 mg (kanchon and Gaurav varieties)/100 g edible portions, respectively. Phytate-zinc molar ratios ranged from 15.3-22.2 in different wheat varieties and 16.9-19.4 in legumes. Balaka variety of wheat contents the highest Phytate-zinc molar ratio (22.2). In this study, estimated Phytate-zinc molar ratio of Bangladeshi daily diet was found to be 12.7.
  Md. Rezuanul Islam , D.K. Paul and Ranajit Kumar Shaha
  Fifteen different kinds of leafy vegetables were investigated for the content of moisture, ash, protein, fat, carbohydrate and dietary fiber. Among all leafy vegetables the lowest value of moisture content in Jute leaves (Corchorus olitorius L.) 76.52 g 100 g-1 and the highest value Pui leaves (Basella alba L.) 92.44 g 100 g-1, respectively. Ash content in leafy vegetables varied between 0.9 to 1.8 g 100 g-1. Among leafy vegetables the lowest value of fibre content in Bengal germ leaves (Cicer arietinum L.) 5.57 g 100 g-1 and the highest value Jute leaves 10.8 g 100 g-1, respectively. From the present results protein, fat and carbohydrate contents in different leafy vegetables varies from 1.67 to 6.5, 0.09 to 1.5 and 3.3 to 12.57 g 100 g-1, respectively. Highest protein, fat and carbohydrate content leafy vegetable is Bengal germ leaves 6.5 g 100 g-1, Drumstick leaves (Moringa oleifera L.) 1.5 g 100 g-1 and Jute leaves 12.57 g 100 g-1, respectively. Among all studied leafy vegetables the highest amount of Neutral Detergent Fiber (47.50 g 100 g-1) was estimated in Radish leaves (Raphanus sativus L.) and the lowest amount of NDF (24.98 g 100 g-1) was contained by Napa leaves (Malva verticillata L.). Cellulose content varied between highest Jute leaves 11.09 g 100 g-1 to lowest Lettuce leaves 4.05 g 100 g-1, hemicellulose content varied between highest Radish leaves 33.90 g 100 g-1 to lowest Napa leaves 15.12 g 100 g-1 and lignin varied between Jute leaves highest 6.01 g 100 g-1 to lowest Radish leaves 0.87 g 100 g-1.
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