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Articles by Rama S. Singh
Total Records ( 2 ) for Rama S. Singh
  Ramandeep Kaur , Jaspal Kaur , Rama S. Singh and C. Alabouvette
  Biocontrol efficacy of selected isolates of nonpathogenic Fusarium and fluorescent Pseudomonas was determined in growth chambers and in microplots. The selected isolates of nonpathogenic Fusarium and fluorescent Pseudomonas were evaluated singly as well as in combination against Fusarium oxysporum f. sp. ciceri (Foc) causing chickpea wilt. Under growth chambers conditions, antagonists were applied as seed and soil treatment while in microplots only seed treatment was done. Pathogenic isolate of F. oxysporum f. sp. ciceri was mixed in soil as soil treatment in all the combinations of antagonists. Under growth chamber conditions, there were 15-30 and 0-15% plants showing wilting after 30 days of sowing with treatment of nonpathogenic Fusarium and fluorescent Pseudomonas, respectively. Gradually the percentage of wilting increased and after 60 days of sowing it was 20-40 and 40-50%, respectively. The combination of Fo52 with C7R12 was the best where none of plants was observed wilted after 30 days of sowing and only 10% plants showed wilting after 60 days. Similar results were obtained from the microplot studies. The seed treatment with Fo52 and C7R12 in combination showed maximum seed germination and disease inhibition as compared to other treatment.
  R. Kaur , J. Kaur and Rama S. Singh
  Fusarium oxysporum is an important fungal group among the soil bone microflora. These strains are well-known for inducing wilt or root rots in important agricultural crops worldwide and some occur only as a saprophytes in rhizosphere of plants. There are certain strains which are nonpathogenic and protect plants from pathogenic strains. Based on phenotypic and genetic studies F. oxysporum showed a great diversity among its populations. The nonpathogenic strains, which were first isolated from suppressive soils strains showed several modes of action against pathogenic strains and thus exploited as biocontrol agents. These nonpathogenic strains suppress pathogens by competing for nutrients in the soil, reduce their chlamydospore germination, compete for infection sites on the root and induce systemic resistance in plant when invade host plant species before the pathogen. The nonpathogenic strains are formulated in talc and charcoal based media and commercial formulations are also available. These strains of Fusarium has been successfully combined with other biocontrol agents to obtain a effective biocontrol of plant pathogens. For application of nonpathogenic Fusarium under field condition some additional research is needed in several areas including: field studies and integration into production systems; risk assessment; and genetic improvement of biocontrol agents.
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