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Articles by Rajeeva Gaur
Total Records ( 4 ) for Rajeeva Gaur
  Rajeeva Gaur , Soni Tiwari and Alka Sharma
  Twelve bacterial strains isolated from the soils sample were screened for protease production on skimmed milk agar. Among them, strain P-02 was adjudged as the best protease producer and was identified as Bacillus sp. The isolate showed maximum protease production (3768 U mL-1) in the presence of wheat bran and peptone at 60°C, pH 9.0 within 48 h of incubation. The enzyme activity was increased in the presence of Ca2+, Mg+2 and Mn+2 ions (0.1%). Protease activity was strongly inhibited in the presence of Hg and Fe ions. This isolate may be useful in several industrial applications owing to its thermo-tolerant and alkali-tolerant characteristics.
  Rajeeva Gaur , Soni Tiwari and Sheffalika Singh
  Fifteen bacterial strains isolated from the dairy soil samples for acidic protease production. Among them, culture RGSS-09 was adjudged as the best protease producer and was identified as Pseudomonas aeruginosa RGSS-09 from MTCC Chandigarh. The isolate showed maximum protease production (1256 U mL-1) with 2.5 g L-1 biomass production in the presence of wheat bran and yeast extract at 37°C pH 6.5 within 48 h of incubation. Pseudomonas aeruginosa RGSS-09 also showed enhanced activity and stability in presence of Ca, Mg, Tween-20, Tween-40, triton-X-100 and SDS at 5 and 10 mM concentration. The enzyme was also stable in the presence of n-dodecane, isooctane, n-decane, xylene, toluene, n-haxane, n-butanol and cyclohexane, respectively. The presence of benzene, methanol and ethanol marginally reduced the protease stability, respectively. The enzyme was showed it 100% activity at 75°C and pH 7.5 with 110 and 100% stability at 70°C and pH 7.0, respectively. The purity of the enzyme was confirmed by the presence of a single band on SDS-PAGE and its molecular weight was approximately 42 kDa. Pseudomonas aeruginosa can be used profitably for the large scale production of protease to meet the present day demand of the industrial sector.
  Soni Tiwari , Rajeeva Gaur and Anurag Singh
  The aim of this study was to isolate a consortium of bacterium and yeast from natural resources for better decolorization of distillery spentwash. Consortium exhibited 82±1.5% decolorization within 24 h when incubated at 45°C under static condition in effluent supplemented with 0.1%, glucose; 0.1%, peptone; 0.05%, MgSO4; 0.01%. The cultures were identified as Pediococcus acidilactici by 16S rDNA analysis and Candida tropicalis on the basis of phenotypic level. It is the first time when thermotolerant melanoidin decolorizing consortium (Pediococcus acidilactici and Candida tropicalis) isolated from distillery soil was capable to decolorizing melanoidin pigment of distillery effluent. Hence, it was observed that consortium has the ability to degrade the spentwash efficiently. This study could be an approach towards control of ecological pollution and health hazards of humans in and about the distillery location.
  Soni Tiwari , Pooja Pathak and Rajeeva Gaur
  Background and Objective: The growing disquiets about the dearth of remnant fuels, the release of green house gasses and air pollution by incomplete incineration of fossil fuel have also resulted in an increasing focus on the use of cellulases to perform enzymatic hydrolysis of the lignocellulosic materials for the generation of bioethanol. The aim of this study was to isolate a potential thermotolerant cellulase producing bacterium from natural resources for the bioethanol production. Materials and Methods: The soil samples were collected aseptically from different site of university campus to isolate cellulase producing bacteria by serial dilution method on CMC agar plates (pH 7.0) at 55°C. Fifty bacterial strains isolated from the soil samples were screened for cellulase production. Among them, two cultures were adjudged as the best cellulase producer and were identified as Streptococcus and Bacillus sp. on the basis of biochemical characterization. After growth, incubated plates were overlaid with congo-red solution (0.1%) for 10 min and then washed with 1 N NaOH solution for de-staining and effective cellulolytic bacterial culture were screened out on the basis of clear zone around the colony for further study. The selected bacterial isolates were identified from MTCC Chandigarh. Data were analyzed by one way ANOVA. Results: The Bacillus sp. showed maximum cellulase production (170 U mL–1) in the presence of sugarcane baggase, ammonium sulphate and Mn2+ ions at 55°C, pH 7.0 within 72 h of incubation while Streptococcus sp. showed maximum cellulase production (130 U mL–1) in the presence of wheat bran, ammonium sulphate and K2+ ions at 50°C, pH 6.5 within 96 h. The enzyme showed maximum activity in the presence of Triton-X-100. Tween-40, Tween-60 and Tween-80 (100 mM), was also found to stimulatory effect, respectively. Conclusion: These isolates (Bacillus and Streptococcus sp.) may be useful in several industrial applications owing to its thermotolerant, heavy metal resistant and surfactant resistance characteristics.
 
 
 
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