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Articles by Rahmat Ali Khan
Total Records ( 2 ) for Rahmat Ali Khan
  Latif Ullah Khan , Rahmat Ali Khan , Samiullah Khan , Syeda Asma Bano , Fehmida Fasim and Bushra Uzair
  Background and Objective: Various parts of medicinal plants have been used to treat specific disorder from ancient times. Swertia chirayita (Roxb. ex Fleming) is a customary folklore medicine, used in the treatment of liver disorders, fevers, dysentery, diarrhea, stomach problems and other disorders. The present study was carried out in order to assess the antioxidant activity, to evaluate the antifungal properties of the plant’s root and to observe anticancer potential of methanolic extract of Swertia chirayita root. Materials and Methods: Phytochemical analysis and different chemical tests for the screening and identification of bioactive chemical constituents in Swertia chirayita methanolic root extract (SCME) were carried out using the standard procedures. The plants were purchased from local herbal market. In vitro determination of antioxidant properties of SCME were conducted using 1,1-diphenyl-2-picrylhydrazyl (DPPH)radical scavenging activity, H2O2 scavenging activity, Beta-carotene bleaching assay, total antioxidant activity by phosphomolybdenum method, azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation scavenging activity and hydroxyl radical scavenging activity. Anticancer activity of SCME was determined according to the protocol of brine shrimp lethality test. Antifungal potential was determined by measuring zone of inhibition on Potato Dextrose Agar (PDA) plates. The different concentrations of SCME used were 3.0, 1.5, 0.75 and 0.37 mg mL–1. The data were evaluated as Mean±Standard Deviations of 5 independent experimental responses. The results were analyzed using t-test for independent samples with SPSS version 16.0. Results: Phytochemical analysis of SCME showed that phlobatannins, tannins, saponins and terpenoids were present. The SCME exhibited strong antioxidant activity in a concentration dependent manner for in all six models. The SCME at dose of 3 mg mL–1 caused 100% death rate of brine shrimp after 72 h. The SCME showed potent activity against Aspergillus flavus (87%) followed by Aspergillus niger (88%) while the highest activity was shown against Aspergillus fumigatus (92%). Conclusion: The SCME exhibited strong antioxidant, antifungal and cytotoxic potential. Purification of different bioactive compounds should be carried out and in vivo studies are required for further verification.
  Wasim Ahmad , Mushtaq Ahmad , Rahmat Ali Khan , Nadia Mushtaq , Jean Paul Kamdem and Joao Batista Teixeira da Rocha
  Background and Objective: Ischemia is a stern decline or absolute obstruction in blood, flowing to various parts of the body. This pathophysiological episode causes cerebral mutilation, a protuberant feature of stroke, which is the 3rd leading cause of demise after cancer and heart attack globally. The principal objective of this work was to understand the sights of neuroprotection provided by M. Officinalis against OGD-R in rat’s brain cortex slices. Materials and Methods: Mitochondrial viability assays were performed via the colorimetric 3(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) method. After 2 h of oxygen and glucose deprivation (OGD) followed by 1 h of reperfusion, only viable slices showed the ability to trim down MTT into a purple "Formazan" product that was soluble in dimethyl sulfoxide (DMSO). Absorbance was measured at 570 and 630 nm and the net absorbance (A570-A630) was taken as an index of cell viability. Results: The results of the present investigation demonstrated that oxygen-glucose deprivation (OGD) followed by re-oxygenation led to cell damage/death via an amplifying ROS/free radicals production in rat’s brain cortex slices compared with control after 2 h OGD followed by 1h reperfusion. Melissa officinalis at a concentration of 40 μg mL–1 displayed potential role in neuro-protection against OGD, followed by re-oxygenation in mitochondrial viability assays in vitro. In addition, Melissa officinalis declined or slow down the production of free radicals in the supernatant and slices homogenate of cortex at the end of 2 h OGD followed by 1 h reperfusion. Furthermore, higher concentrations of Melissa officinalis slightly showed neurotoxicity for cortex slices which might be attributed to its pro-oxidant outcome. Conclusion: The results obtained during this study offer evidence for neuroprotective properties of M. officinalis against in vitro ischemia in rat’s cortex slices. Melissa officinalis could be considered as a therapeutic agent in the prevention of neuronal cell death in Ischemia induced by oxygen and glucose deprivation of cortex slices, strengthening further investigations to define the actual component for its use in human. Furthermore, in vivo ischemic models are now in progress to confirm and better characterize its neuroprotection.
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