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Articles by Rahman Md. Mukhlesur
Total Records ( 3 ) for Rahman Md. Mukhlesur
  Rahman Md. Mukhlesur and Yutaka Hirata
  Eighty five different cultivars of Brassica rapa, B.juncea, B.napus, B. carinata, B. oleracea and hexaploid Brassica, collected from Bangladesh, Japan, China and Denmark, were analyzed for seed and leaf protein variations by SDS-PAGE to identify the polymorphic genetic markers for evaluation of genetic resources. Ten polymorphic markers were identified from seed protein and no identifiable polymorphic band was found from leaf protein. However, polymorphic markers clearly distinguished these Brassica species. Brassica rapa var. `yellow sarson` of Bangladesh origin showed uniquely identifiable four polymorphic bands for seed protein in contrast to the other B.rapa of brown-seeded type. The Bangladeshi and Japanese cultivars of B. rapa differed among protein quantity. Analytical results of SDS-PAGE for seed protein showed that hexaploid Brassica has the highest indices, such as % of polymorphic band, the degree of phenotypic diversity (Ho), diversity value for genetic marker (HEP) and the sum of the effective number of alleles (SENA). The genetic diversity values of hexaploid Brassica were followed by amphidiploid (B. napus, B. juncea, B. carinata) and diploid (B. oleracea, B. rapa) species, respectively.
  Rahman Md. Mukhlesur , Yutaka Hirata and Shah-E-Alam
  Genetic relationship were evaluated among thirty two cultivars of Brassica rapa, collected from Bangladesh, Japan and China, by employing SDS-PAGE for seed protein and esterase, acid phosphate and peroxidase analysis. SDS-PAGE generated a total of 32 bands of which nearly 31% were polymorphic, followed by esterase of 9 bands of nearly 19% polymorphic and no identifyable polymorphic band was found from both acid phosphate and esterase analysis. However, these polymorphic markers clearly distinguished between yellow sarson and brown seeded cultivars. Cluster analysis using data generated by SDS-PAGE for seed protein, clearly separated the yellow sarson, self-compatible cultivars from the brown seeded, self-incompatible cultivars, as well as different brown seeded cultivars from Bangladesh, Japan and China origin.
  Rahman Md. Mukhlesur and Yutaka Hirata
  Polymerase Chain  Reaction (PCR) was carried out with seed coat color specific SCAR marker of B. juncea. The yellow-seeded cultivars were collected from Japan and Denmark, where the brown-seeded cultivars were collected from Bangladesh and Japan. PCR products were visualized in 1% agarose gel, where the yellow-seeded cultivars produced a strong band at 0.5 kb and weak band at 1.2 kb. In addition of these two specific bands, the Japanese yellow-seeded cultivars expressed two more week bands at 1.1 and 1.0 kb. The brown-seeded cultivars generated a single strong band at 1.1 kb. In segregating population of a cross of yellow-seeded and brown seeded cultivars, the yellow-seed coat color marker segregated at a ratio 15 (brown): 1 (yellow), indicating the digenic inheritance pattern of the traits. PCR was also carried out with the same primers to the yellow-seeded and brown-seeded cultivars of B. rapa. It was interesting to note that the yellow-seeded cultivar of B. rapa did not show any band, where the brown-seeded cultivar of B. rapa showed bands at 1.1 kb and 0.5 kb. The result indicates that the seed coat color specific primer of B. juncea has also ability to differentiate the yellow-seeded and brown-seeded cultivars of B. rapa. PCR-Southern hybridization was carried out with 0.5 and 1.1 kb fragments as prove to check the homology of the other amplified fragments. The prove DNA fragments (0.5 or 1.1 kb) hybridized all other DNA fragments at 1.2, 1.1, 1.0 and 0.5 kb of B. juncea and brown-seeded B. rapa. The result indicating that all the mentioned fragments bearing a common homology region of DNA of yellow-seeded B. juncea and brown-seeded cultivars of B. rapa.
 
 
 
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