Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by Rahmah Mohamed
Total Records ( 6 ) for Rahmah Mohamed
  Lai L. Suang , Zamberi Sekawi , Nagi A. Al-Haj , Mariana N. Shamsudin , Rasedee Abdullah and Rahmah Mohamed
  Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study, by using the available B. pseudomallei genome database provided by Wellcome. The study demonstrated that the bioinformatics comparative technique was able to annotate LPS genes in Burkholderia pseudomallei. By developing a simple and easy flow chart including the using of Artemis software, total of 44 putative ORFs involved in biosynthesis of lipopolysaccharide for B. pseudomallei and the genetic mapping for the ORFs have been successfully determined using bioinformatics and laboratory approach. It is about 95.7% of success for annotation based on the 46 genes that act as references. In near future, a suitable vaccine or antimicrobial may be developed by targeting the genes encoding the various components essential in LPS biosynthesis and survival of the pathogen.
  Lai L. Suang , Zamberi Sekawi , Nagi A. Al-Haj , Mariana N. Shamsudin , Rasedee Abdullah and Rahmah Mohamed
  Recently several cases of melioidosis have been reported in the tropical climates, especially in Southeast Asia where, it is endemic, it also occurs sporadically throughout the world. The diagnosis of the acute or chronic infection remains challenging. The present study highlight on the optimized and reliable technique based DNA preparation for use in Polymerase Chain Reaction (PCR) assay. PCR amplification with specific pair of primer for each putative gene was proving specific for amplification of genes in Burkholderia pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. seudomallei specifically for strain D286. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis.
  Nagi A. Al-Haj , Lai L. Suang , Mariana N. Shamsudin , Rasedee Abdullah , Rahmah Mohamed and Zamberi Sekawi
  Burkholderia pseudomallei is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of B. pseudomallei infections may cause by Lipopolysaccharides (LPS), an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study was designed to further elucidate genes involved in the biosynthesis pathway of LPS in melioidosis agent followed with selected gene product expression with essential function for survival and virulence melioidosis agent. Expression of Bplps0013/lpxA and Bplps0007/rfaF successful expressed the entire proteins in 2 h with sizes of approximately 29 kDa and 43.7 kDa, respectively. The baseline information provided through the present research can be a preliminary approach towards the development of effective therapeutics against melioidosis.
  Nagi A. AL-Haj , E. Amghalia , Mariana N. Shamsudin , Rasedee Abdullah , Rahmah Mohamed and Zamberi Sekawi
  Methicillin-resistant Staphylococcus aureus strains have appeared in countries worldwide and continue to be one of the most common hospital pathogens and it has become increasingly prevalent in community-acquired infections and provided strong evidence for the independent origins of health care-associated Methicillin-resistant Staphylococcus aureus and community-acquired. It has been shown that methicillin-susceptible S. aureus strains become MRSA strains by the acquisition of a staphylococcal cassette chromosome mec element carrying the mecA gene, which is responsible for methicillin resistance and has become essential for the characterization of Staphylococcus aureus clones in epidemiological studies. The objective of this study to identify the staphylococcal cassette chromosome mec types of methicillin-resistant Staphylococcus aureus isolated from different Malaysian Hospitals. PCR amplification and sequencing analysis were performed to determine the SCCmec type of MRSA. The present research successfully established molecular characteristics of local MRSA contribute as initial database of these isolates in order to fully understand the epidemiology, microbiology and pathophysiology of these infections.
  Nagi A. AL-Haj , E. Amghalia , Mariana N. Shamsudin , Rasedee Abdullah , Rahmah Mohamed and Zamberi Sekawi
  For centuries honey had a valued place in traditional medicine, being used in the treatment of wounds and diseases of the gut. The scientific community has now rekindled interest in the therapeutic use of honey in modern medicine and a number of published reports support its use in certain medical conditions, including burns and wounds. The aim of the present study to the effectiveness of the antimicrobial activity of honey against Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin-Sensitive Staphylococcus aureus (MSSA) isolates collected from various Malaysian hospitals. Thirty isolated of Staphylococcus aureus were found to be resistant to routinely used higher antibiotics. Using an agar incorporation technique the sensitivity of these strains to honey was tested by the method of minimum inhibitory concentration. All the tested strains of Staphylococcus aureus showed inhibition with honey at concentrations of 25 and 30%. The present study recommended that the multidrug-resistant Staphylococcus aureus infection particularly wound and burns honey may be useful for controlling infection.
  Mohd Firdaus-Raih , Roohaida Othman and Rahmah Mohamed
  The tropical soil pathogen Burkholderia pseudomallei is known to secrete a variety of extracellular virulence factors and is resistant to a wide range of antibiotics. ATP Binding Cassette (ABC) transport systems typically consist of three proteins encoded by genes in the same operon or neighboring operons. This functionally diverse protein super family typically carry out vector driven transport across cell membranes. The studies have identified an open reading frame that was predicted to code for an ATP binding protein in B. pseudomallei. A whole genome inventorization and function classification by sequence similarity clustering was done by comparison of ABC transporters from three completed genomes. The predicted protein sequence of the ATP binding domain was successfully modelled onto the crystal structure of an ATP binding subunit for the histidine permease of Salmonella typhimurium. The putative ATP binding site was identified and the model deemed to be functionally viable from a structural-mechanics point of view. We have also detected two highly conserved residues which appear to be independent of the known motifs associated with the ATP binding subunit. This integrated process was able to identify and infer function to an ABC domain from B. pseudomallei despite low target template sequence identity (26.4%). This approach enabled ABC transporters with varying functions to be classified predictively.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility