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| Articles
by
Raha Abdul Rahim |
Total Records (
2 ) for
Raha Abdul Rahim |
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Lesley Maurice Bilung
,
Son Radu
,
Abdul Rani Bahaman
,
Raha Abdul Rahim
,
Suhaimi Napis
,
Cheah Yoke Kqueen
,
Chandrika Murugaiah
,
Yousr Abdul Hadi
,
Tunung Robin
and
Mitsuaki Nishibuchi
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Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains. |
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Nagi A. AL-Haj
,
Mariana Nor Shamsudin
,
Raha Abdul Rahim
,
H. Halimaton
,
Lai L. Suang
,
M. Nurmas I. Mashan
and
Zamberi Sekawi
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Vibrio cholerae has caused severe outbreaks of cholera
worldwide with thousands of recorded deaths annually while the Staphylococcus
aureus is one of the most significant pathogens causing nosocomial and community-acquired
infections. Conventional detection methods for diagnosis of clinical samples,
water and food based on culture, microscopy and biochemical testing are limited
by the speed of detection, sensitivity and specificity, so it is necessary to
develop innovative molecular methods for the rapid detect the presence genes,
expression levels of the toxigenic and drug target genes in S. aureus
and V. cholerae using PCR, sequencing and membrane array. The genes studied
are SEA-SEJ (genes encoding S. aureus enterotoxins) ace, zot,
ctxA, ctxB, toxR (toxigenic genes of V. cholerae)
Sav1017 and AdaB (protein synthesis and DNA synthesis genes in
S. aureus. These techniques were carried out step by step with primers
designing, PCR amplification, sequencing and detection of expression by membrane
array. These assays are extremely robust, sensitive, specific and economical
and can be adapted to different throughputs. Thus, a rapid, sensitive and reliable
technique for detecting toxigenic genes of S. aureus and V. cholerae
was suc-cessfully developed. |
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