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Articles by Ragaa A. Hamouda
Total Records ( 5 ) for Ragaa A. Hamouda
  Khalid Abd EL Ghany , Ekbal Abd Elhafez , Ragaa A. Hamouda , Hoda Mahrous , Fattah Allah H. Ahmed and Hanafy A. Hamza
  Probiotic bacteria are considered beneficial microorganisms and have been widely used. They protect against harmful bacteria that can cause diseases. This study was carried out to evaluate the potential for L. acidophilus intact cells and cell lysates as antioxidant activities and also to evaluate the potential of this bacteria and their exopolysacchariedes (EPS) as antitumor activity in vitro and in vivo against Ehrlich Ascites Carcinoma (EAC) cell line. Lactobacillus acidophilus demonstrated having antioxidant activities for both cell lysates and intact cells with increasing antioxidative properties for cell lysates against 2, 2--diphenyl-1-picrylhydrazyl (DPPH), also resistance to hydrogen peroxide and hydroxyl radical. The EPS produced by L. acidophilus showed a powerful antitumor effect in vivo and in vitro in comparison with L. acidophilus itself while, E. coli increasing solid tumor volume than control.
  Shymaa Kamal , Ragaa A. Hamouda , Hoda Mahrous , Mohammed Labib Salem , Hanafy A. Hamza and Ekbal Abd Elhafez
  Increase in the levels of cholesterol is harmful, it is a risk factor for cardiovascular disease. This study was carried out to compare the in vitro anti-hypercholesteremia effects of different concentrations of probiotic bacteria (lactobacillus casei and lactobacillus plantarum) and cyanobacteria (Spirulina platensis) isolated as intact cells or cell lysates. The results showed that S. platensis is more effective The mixture of cell lysates from L. casei, L. plantarum and Spirulina platensis lowered the level of cholesterol by 65% as compared to the inhibitory effect of L. casei (32%), L. plantarum (33%) and S. platensis (47%). Their significant differences by using the mixture of testing bacteria as intact cells and cell lysate in the percentage of cholesterol reduction.
  Khalid Abd El Ghany , Ragaa A. Hamouda , Hoda Mahrous , Ekbal Abd Elhafez , Fath Allah H. Ahmed and Hanafy A. Hamza
  Background: Lactic Acid Bacteria (LAB) have great therapeutic and nutritional benefits. Among these are: Improved nutritional value of food, control of intestinal infections, improved digestion of lactose, immune modulator effect, control of some types of cancer and control of serum glucose level. Objective: The aim of this study was carried out to evaluate the therapeutic effect of β-glucan extracted from LAB was isolated from different food sources in Egypt. Materials and Methods: The isolation was performed from local Egyptian boza, cider, cheese and yoghurt. Only seven species from 27 was identified as LAB. Only one showed to produce β-glucan and identified by VITEC® 2 as Pediococcus parvulus . The optimization of isolate growth was examined in different temperature, pH and media. Results: The study revealed that the optimum temperature was 37°C, pH was 6 and the most selective medium for growth was Pediococcus selective medium (PSM). Beta-glucan production was assessed by FT-IR analysis, HPLC and HNMR spectroscopy. The therapeutic effect of Pediococcus parvulus β-glucan as antioxidant against DPPH was more significant, cholesterol lowering effect of β-glucan gave valuable results compared to simvastatin drug, the antibacterial effect and control of cancer in vitro against Ehrlich Ascites Carcinoma (EAC) were also gave valuable results. Conclusion: The extracted β-glucan from Egyptian Pediococcus parvulus had different therapeutic effect in vitro including, antioxidative properties, antimicrobial, control of cancer and cholesterol lowering effect.
  Badr E. El Bialy , Ragaa A. Hamouda , Khaled S. Khalifa and Hanafy A. Hamza
  Objective: This study aimed to evaluate the potential anti-cancer effect of silver nanoparticles (AgNPs, of 8-16 nm size range) biosynthesized from Turbinaria turbinata marine-alga against Ehrlich Cell Carcinoma (ECC) in mice. Materials and Methods: After 1 day of s/c Ehrlich cells inoculation in mice thigh, AgNPs were injected in the same site at doses of 85 and 42.5 μg for each mouse daily for 20 days. Till the end of the experiment, none of clinical signs were observed on mice. Results: The AgNPs induced dose-dependent reduction in tumor size. The cytotoxic insult of AgNPs against ECC referred to its potential oxidative damage effect proved by elevation of MDA and H2O2 contents in tumor tissue and induction of apoptosis via caspase 3 activation. Also, AgNPs brought the elevated white blood cell count in tumor-bearing mice to near-normal range. The histopathological investigation of tumor tissues revealed presence of severe necrosis of cancer cells especially at higher dose of AgNPs. Conclusion: Biosynthesized AgNPs from Turbinaria turbinata could control the growth of ECC in mice with limited adverse effects.
  Ragaa A. Hamouda , Noura El-Ahmady El-Naggar and Ghada Wagih Abou-El-Seoud
  Background and Objective: The growth rate, primary and secondary metabolites are influenced by nitrogen concentration of media. This study aimed to determine the effect of different concentrations of KNO3 as a source of nitrogen on the growth, phytochemical components, cholesterol reduction, antioxidant and antibacterial activities of the green alga, Scenedesmus obliquus (S. obliquus). Materials and Methods: Scenedesmus obliquus was cultured in Kuhl’s medium and KNO3 was added with different concentrations in the medium at different concentration (0.12, 0.75, 1.5, 2.25 and 3 g L–1). The effect of different five concentrations of nitrogen on protein and carbohydrates was determined. Antioxidant activity, total phenolic content (TPC) of alga extracts, cholesterol reduction and antibacterial activity were evaluated. The data analyses were carried out using SPSS software version 16. Results: The results revealed that the best KNO3 concentration for algal growth and carbohydrate content is1.5 g L–1. Meanwhile, the high lipid content was obtained with KNO3 starvation. Medium containing 0.75 g L–1 of KNO3 has the highest effect on the protein production of S. obliquus. The contents of dry alga extracted with chloroform: methanol and the extracted contents were determined by GC/MS chromatogram; the major contents were 5-Hydroxymethylfurfural followed by hexadecanoic acid (palmitic acid), cis-9-octadecenoic acid (oleic acid) and hexadecanoic acid and methyl ester, respectively. Conclusion: Potassium nitrate limitation caused the highest effect on the total phenol content (TPC), increased antioxidant capacity, cholesterol reduction activity and also antibacterial activity against Staphylococcus aureus and Escherichia coli.
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