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Articles by R.S. Sultana
Total Records ( 6 ) for R.S. Sultana
  Ahsan Nagib , S.A. Hossain , M.F. Alam , M.M. Hossain , R. Islam and R.S. Sultana
  In order to produce virus free potato tuber seeds in vitro culture was established using apical meristem of four widely cultivated varieties of Bangladesh namely Diamant, Cardinal, Multa and Lalpakri. Meristem of these varieties of potato were cultured for shoot proliferation and root induction in MS and MS0 medium supplemented with different types and concentration of phytohormones. Among the different growth regulator formulations, 0.5 mg l-1 GA3+0.04 mg l-1 KIN was found to be the best medium for the primary establishment of meristem. The primary established meristems were subcultured on to MSo medium and MS medium containing BA and IBA singly or in combinations. Considering all the treatments singly use of IBA (0.5 mg l-1) is recommended for proper shoot and root development from primary meristem. After DAS-ELISSA test the virus free in vitro grown potato plantlets were using for massive micro propagation. GA3 and KIN singly or in combinations are used for shoot and root multiplication, among all these combinations 2.0 mg l-1 GA3 was found to be the best media for shoot induction for the studied varities. For high frequency of root formation, combination of GA3 (0.1 mg l-1)+KIN (0.1 mg l-1) was most effective. Rooted plantlet were gradually acclimatized and successfully established in the field. Visual evolution of the morphological trials of the tissue culture derived plants showed all plants were found normal and free from virus diseases. Substantial yield increase was observed in meristem derived plants over their source plants.
  R.S. Sultana , M.A. Bari , M.H. Rahman , M.M. Rahman , N.A. Siddique and N. Khatun
  Plantlets regeneration were achieved in callus culture of leaf segments from five days old field grown seedlings of Citrulus lanatus Thumb. Callus induction and plant regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed in MS medium supplemented with 2.5 mg L-1 2, 4-D. After transfer of this callus on MS medium supplemented with 1.0 mg L-1 BA+0.2 mg L-1 NAA induce adventitious shoots and developed into plant by further subculture in the same medium. NAA (0.1 mg L-1) was found effective in the production of root. Plantlets were acclimatized and subsequently transferred to the field. Survival of the plantlets under ex vitro condition was 80%.
  R.S. Sultana and M.A. Bari Miah
  An efficient protocol was developed for direct plant regeneration, multiplication and rooting in in vitro condition of Momordica charantea Linn. locally known as "karalla". Nodal segments and shoot tips from in vitro grown seedling were cultured on MS medium supplemented with different concentrations and combinations of cytokinins (BA, KIN) and auxins (NAA, IAA) for direct shoot regeneration. Best response towards multiple shoot regeneration was obtained from the nodal segments of Momordica charantea Linn. on MS medium supplemented with 2.0 mg l G 1 BA + 0.2 mg l G 1 NAA. In this combination, the frequency of multiple shoot regeneration response from nodal segments was 95% and average number of shoots per explant was 8.20 " 0.26. The length of the longest shoot was 5.20 " 0.21 for nodal segments cultured on same combination. Efficient rooting was achieved on half strength of MS + 0.5 mg l G 1 NAA. About 90% of the regenerated plantlet survived in the field condition.
  R.S. Sultana , M.A. Bari Miah , M.M. Rahman , M.H. Rahman and M.U. Mollah
  Effects of different concentrations of sucrose, agar and different levels of pH on in vitro axillary shoot multiplication of Momordica charantea Linn. were studied in the present investigation. The nodal segments from the field grown plant were used as testing plant material. For maximum number of shoot induction and multiplication in MS medium containing 2.0 mg L 1 BAP+0.2 mg L 1 NAA with 30 g L 1 of sucrose, 7 g L 1 of agar and 5.5-6.0 levels of pH proved more effective. When the medium having 30 g L 1 sucrose, the highest percentage of explant responded to shoot proliferation and that was 100%. This sucrose concentration also showed the optimum result for total number of shoot per culture and average length of shoots and that were 5.10.8 and 5.60.4 cm, respectively. The highest response of shoot proliferation from the nodal explant was observed on MS medium having 7 g L 1 of agar and the frequency was 100%. Among different levels of pH, the highest percentage of explant showing proliferation was observed on the media adjusted to pH 5.5-6.0 levels. It was proved by the present investigation that in vitro growth and shoot multiplication was affected by sucrose, agar and pH on the shoot induction medium.
  A.N.K. Mamun , M.N. Matin , M.A. Bari , N.A. Siddique , R.S. Sultana , M.H. Rahman and A.S.M. Musa
  The woody legume, Albizia lebbeck, commonly cultivated for social forestry in rural area of Bangladesh was selected for micropropagation by tissue culture technique. Cotyledon, nodal segment of in vitro grown seedlings and nodal segments of field grown mature tree were used as explants. With proper manipulation of cytokinin and auxin combinations and concentrations, it was possible to induce callus from three types of explants of Albizia lebbeck. Different combinations of BA with NAA and KIN with NAA were proved efficient media formulation for callus induction. Among all the hormonal supplements used BA-NAA combination with MS medium was proved best in all respect of callusing response in Albizia lebbeck. Among the explants the in vitro internodal segments were the best for callus induction. The highest 100% callus induction was observed in media having MS+2.0 mg L-1 BA+0.2 mg L-1 NAA and the fresh weight of callus was 1.3 g. Among these three explants of Albizia lebbeck only calli derived from cotyledon regenerated best shoots in MS media supplemented with BA singly or in combination with NAA and KIN. In respect of direct shoot regeneration nodal explants of Albizia lebbeck produced highest range of regenerated shoots.
  M.Z. Rahman , M.H. Rahman , M.U. Mullah , N. Nahar , R.S. Sultana , M.A. Bari and M. Hossain
  The effects of various cytokinins (viz., BAP and KIN), auxins (viz., IBA, NAA and IAA) and coconut water (CW) alone or in combinations on shoot multiplication of banana cv. Anupom were investigated. The rate of multiplication varied in different treatments. Among the treatments, MS medium supplemented with 5.0 mg L-1 each of BAP + KIN and 13% coconut water produced the highest number of shoot per explant (5.8±0.154). The number of shoots responded for rooting and their survivability were found higher with IBA than NAA and IAA. MS medium supplemented with 1.0 mg L-1 IBA produced the highest number of roots per shoot (7.±0.245). Plantlets grown without any auxin in the medium gave the least number of roots/shoot (2.60±0.219). The rooted plantlets were successfully transferred in the polybags and finally were well established in the field.
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