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Articles by R.S. Singh
Total Records ( 3 ) for R.S. Singh
  R. Kaur , R.S. Singh and C. Alabouvette
  The antagonistic activity against in vitro growth of Fusarium oxysporum f. sp. ciceri was determined for 90 isolates florescent Pseudomonas obtained from the rhizosphere and rhizosphere of healthy, partially wilted and completely wilted chickpea. Based on zone of inhibition, isolates were categorized into four categories, i.e. highly, moderately, least and non-antagonists. Maximum number of highly antagonistic isolates was obtained from rhizosphere and rhizoplane of healthy chickpea plants, whereas, maximum number of non-antagonistic isolates were obtained from completely wilted chickpea plants. Isolate H-Pf5 and C7R12 were selected for green house and field bioassays. Cell free culture filtrate of both the selected isolates was able to inhibit conidial germination of pathogen. Under green house and field conditions the isolates of fluorescent Pseudomonas, significantly enhanced seed germination, reduced disease incidence and promoted plant growth of chickpea as compared to control.
  R.S. Singh , B.S. Sooch , Kamaljit Kaur and J.F. Kennedy
  The present study was carried out at flask level for the fermentative production of citric acid from cheddar cheese whey using a yeast culture Metschnikowia pulcherrima NCIM 3108. Clarified and unclarified whey with and without nutrient supplementation was screened for citric acid production. The order of citric acid production was supplemented unclarified whey>supplemented clarified whey>unclarified whey>clarified whey. The effect of various process parameters was investigated to maximize the citric acid production from supplemented unclarified whey. The lactose concentration of 6.0% (w/v) at pH 6.0 with an inoculum size 10% (v/v) of 48 h old culture at 30°C supported the maximum concentration of citric acid production after 5 days of incubation period. The agitation of the yeast culture suppressed the citric acid production to a little extent as compared to stationary conditions.
  R. Panesar , P.S. Panesar , R.S. Singh , J.F. Kennedy and M.B. Bera
  The use of Kluyveromyces marxianus NCIM 3465 for the production of ß-D-galactosidase is reported. Experimentation was carried out to optimize the process parameters for maximum enzyme production by varying the parameters such as pH of the medium, temperature, inoculum size, age of inoculum, agitation and incubation time. The maximum enzyme activity was observed with pH 5.0, temperature 30°C, inoculum size 6% (v/v) having 20 h age, under shaking conditions (100 rpm) after 24 h of incubation.
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