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Articles by R.J. Buhr
Total Records ( 2 ) for R.J. Buhr
  J.A. Cason , R.J. Buhr , L.J. Richardson and N.A. Cox
  External and internal persistence of inoculated Salmonella and spread to uninoculated broiler chicks in the same pens were studied by sampling ceca and rinses of feathered carcasses in two experiments. Half of the day-old chicks in pens were orally inoculated with a nalidixic-acid-resistant strain of Salmonella Typhimurium at three levels of inoculum (0.1 mL delivering approximately 4 X 102, 104, or 106 cfu). At 3, 6, and 8 weeks of age, equal numbers of inoculated and non-inoculated pen mates were individually electrocuted and rinsed in 400 mL of diluent, after which ceca were removed aseptically, with a total of 654 chickens sampled in the two experiments. There were no differences in Salmonella incidence between inoculated and non-inoculated birds at any age, so the marker Salmonella was well distributed within pens. Total incidence was 70%, 86%, and 83% at the 102, 104, and 106 inoculum levels, respectively. Considering both cecal and rinse samples, incidence was 81%, 84%, and 72% at 3, 6, and 8 weeks of age respectively. There were 95 positives in the cecal samples only, 149 positives in the rinses only, and 277 positives in both ceca and rinse samples, so sampling either ceca or carcass rinses alone underestimated the total incidence of the marker Salmonella.
  A. Jr. Hinton , J.A. Cason , R.J. Buhr and K. Liljebjelke
  Effects of spray washing carcasses with Lauric Acid (LA)-Potassium Hydroxide (KOH) on bacteria recovered from Whole-Carcass Rinsates (WCR) were examined. Carcass skin was inoculated with antibiotic resistant strains of Escherichia coli, Salmonella Typhimirum and Campylobacter coli. The first trial examined effects of washing carcasses with water, 0.25% LA-0.125% KOH, 0.50% LA-0.25% KOH, 1.00% LA-0.50% KOH, or 2.00% LA-1.00% KOH at 80 psi for 15 sec. Findings indicated that significantly fewer Total Plate Count (TPC) bacteria, E. coli and Salmonella Typhimirum were recovered from carcasses washed with 2.00% LA-1.00% KOH than from carcasses washed with water and that no C. coli were recovered from carcasses washed with 2.00% LA-1.00% KOH. Another trial examined effects of washing carcasses at 60, 100, or 150 psi of pressure with 2.00% LA-1.00% KOH for 15 sec. Findings indicated that significantly fewer TPC bacteria were recovered from rinsates of carcasses washed with 100 psi than from carcasses washed with 60 or 150 psi. Finally, a trial was conducted to examine effects of washing carcasses for 0, 5, 15, or 30 sec with 2.00% LA-1.00% KOH at 100 psi. Results indicated that significantly fewer bacteria were recovered from carcasses washed for 5 sec than from unwashed carcasses. Furthermore, significantly fewer TPC bacteria and Salmonella Typhimirum were recovered from carcasses washed for 15 sec than for 5 sec and no C. coli were recovered from carcasses washed for 15 or 30 sec. Findings indicate that spray washing carcasses with LA-KOH can affect the number of bacteria recovered from WCR. These studies also provide data that may be useful in designing applications for using of microbicidal surfactants in processing operations.
 
 
 
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