Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by R.B. Rai
Total Records ( 3 ) for R.B. Rai
  A. Hansa , R.B. Rai , M. Yaqoob Wani and K. Dhama
  Bovine coronavirus (BCoV) is widespread in cattle population, resulting in heavy economic losses to both dairy and beef industry throughout the world. The syndromes associated with BCoV include winter dysentery in adult dairy cattle and respiratory and intestinal tract infections in young calves. The virus has specific tropism for intestinal and pulmonary epithelial cells. Reports regarding prevalence and molecular detection of BCoV from India are scarce. In this study, 101 fecal samples were collected from clinical cases of diarrheic calves from North Indian region covering three dairy farms of Uttar Pradesh and dead calves of post mortem facility of Indian Veterinary Research Institute. Fecal samples from all the cases were screened for the presence of BCoV by commercially available ELISA kit. Furthermore, all samples were subjected to RT-PCR for detection and confirmation BCoV. RT-PCR was carried out using two different sets of primers to amplify the conserved nucleocapsid (N) gene of the virus targeting a 407 and 730 bp fragments. An incidence rate of more than 14% (15/101) was observed with ELISA and about 20% (20/101) by RT-PCR. The present report is first in its nature regarding the detection of BCoV at molecular level in India. In conclusion, RT-PCR was found more sensitive than commercial ELISA kit for detecting BCoV in fecal samples. Further extensive epidemiological studies are suggested for the virus in the country to know the magnitude of BCoV infection in dairy calves along with isolation of viral strains and to investigate their antigenic and genetic properties.
  M. Saminathan , R.B. Rai , K. Dhama , G.J. Ranganath , V. Murugesan , K. Kannan , S. Pavulraj , A. Gopalakrishnan and C. Suresh
  Mammary tumours rank second as the most common neoplasms in dogs after skin tumours, whereas in women the most common cause of cancer-related deaths is breast cancer. N-Methyl-N-Nitrosourea (NMU) is a highly specific mammary gland carcinogen which directly act and does not require metabolic activation. In the present study, NMU at the dose rate of 50 mg kg-1 body weight was used intra-peritoneally for the induction of mammary tumour. The first palpable tumour appeared on 70th day post carcinogen injection and subsequently, most of the tumours were developed around 18-20th week. During 28 weeks of experimental period, the tumour incidence was 82.86% (29/35). The tumour frequency was found to be 4.7±0.33 tumours and the average latency period was 107±4.1 days. The average tumour volume was found to be 69±8.8 cm3. Equally, 50% of mammary tumours appeared on the right (22/44) and left (22/44) mammary gland chain. Region wise, 81.82% (36/44) of the tumours appeared on abdominal-inguinal mammary glands and 18.18% (8/44) on the cervical thoracic mammary glands. A total of 44 mammary tumours were diagnosed in which 88.64% (39/44) were malignant and 11.36% (5/44) were benign. Among the malignant tumours, 33.33% (13/39) were non-invasive and 66.67% (26/39) were invasive. The average values of mitotic index, Proliferating Cell Nuclear Antigen (PCNA), Vascular Endothelial Growth Factor (VEGF) and Platelet Endothelial Cell Adhesion Molecule (PECAM-1) in NMU induced mammary tumours were found to be 4.5±0.46/hpf, 77±2.6, 16.2±0.86 and 15±0.69, respectively. The present study for the first time demonstrated the expression of VEGF and PECAM-1/CD-31 proteins in NMU induced mammary tumours.
  A. Hansa , R.B. Rai , K. Dhama , M.Y. Wani , M. Saminathan and G.J. Ranganath
  Bovine Coronavirus (BCoV) is widespread both in dairy and beef cattle throughout the world. The virus is one of the largest RNA virus and has specific tropism for intestinal and pulmonary epithelial cells. It is responsible for huge economic losses by causing winter dysentery in adult dairy cattle and respiratory and intestinal tract infections leading to pneumo-enteritis in young calves. Isolation of BCoV has been reported to be difficult. Studies regarding epidemiology, virus isolation and molecular detection from India are very few. In the present study Vero cell line was used for isolation of the BCoV from Enzyme Linked Immunosorbent Assay (ELISA) positive samples. Direct florescent antibody technique (dFAT) and reverse transcriptase-polymerase chain reaction (RT-PCR) were used to confirm the isolated virus strains at antigenic and genomic levels, respectively. Out of the 15 positive fecal samples, virus from only seven was able to infect vero cell line. Subsequently BCoV got adapted to the vero cell line upto three passages, which was confirmed both at genomic and antigenic levels by dFAT and RT-PCR testing. It can be concluded that vero cell line can be used for isolation of BCoV, however due to the enormous stain diversity of the virus it is possible that many stains can’t grow and get adapt in this cell line. Further studies are required for isolation of different viral strains, finding the susceptible cell lines and also to confirm the variations among the BCoV isolates at antigenic/genomic levels.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility