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Articles by R.A. Onyeagba
Total Records ( 2 ) for R.A. Onyeagba
  E.S. Amadi , A. Oyeka , R.A. Onyeagba , I. Okoli and O.C. Ugbogu
  The antimicrobial effect of cold water, hot water and ethanolic extracts of Spondias mombin and Baphia nittida on cariogenic streptococci isolated from dental caries patients attending the Ebonyi State University Teaching hospital dental clinic Abakaliki was investigated using the agar well diffusion technique. The cold water and ethanolic extracts of Baphia nittida showed inhibition zone diameter (IZD) of 10 and 12 mm respectively at 400 mg mL -1, while the hot water showed no inhibitory effect. All extracts of Spondias mombin did not inhibit the test organism. The cold water and ethanolic extracts of Baphia nittida showed Minimum Inhibitory Concentration (MIC) of 100 and 50 mg mL -1 respectively. The combination of the cold water extracts of the two herbs showed enhanced activity of 13 mm. Phytochemical analysis of Baphia nittida revealed the presence of flavonoids, glycosides, proteins saponins, tannins, carbohydrate and steroidal aglycone. Acute toxicity testing of Baphia nittida at a range of 250-5000 mg kg -1 bw using mice showed no clinical signs of acute toxicity. No chemical toxicity was observed amongst rats given Baphia nittida extracts 500 and 1000 mg kg -1 bw after 30 days. Baphia nittida may be a potential source of an antimicrobial agent for the treatment and management of dental caries.
  V.O. Nwaugo , R.A. Onyeagba , G.N. Chima and D.I. Agwaranze
  The effects of drilling wastes on the farmland microbial spectrum in Egbema, a community in Niger Delta area of Nigeria was investigated. Bacterial species in 12 genera were isolated and include Escherichia, Klebsiella, Pseudomonas, Xanthomonas, Alcaligenes, Bacillus and Staphylococcus. Others are Serratia, Flavobacterium, Micrococcus and Nitrobacter species. All the organisms had their lowest prevalence nearest the waste pit (10 m), followed by 50 m away while there was no significant difference between the 100 m and control samples (p = 0.05) during the dry season. In rainy season, the prevalence rates were similar for 10 and 50 m but significantly different from 100 m and control points. The bioload of the various groups of organisms investigated followed the same pattern i.e., 10 m > 50 m > 100 m > control. While the least affected group was the sulphur reducing bacteria, the most affected were Nitrifying bacteria. Time and distance influenced soil pH as it increase with increase in distance (towards alkalinity) but decreased with time (toward acidity). The highest temperature was observed nearest the waste pit (10 m) and the lowest occurred further away (100 m) but increased with time. Change in pH and temperature indicated metabolism of the drilling wastes as observed in the screening of isolates for chrome-lignoso/fonate utilization. Turbidity, lowering of pH and increase in temperature were observed in chrome-lignoso/fonate utilizing bacterial species as seen in Pseudomonas, Alcaligenes and Serratia species. This was followed by Micrococcus, Staphylococcus and Bacillus species, while Xanthomonas, Citrobacter, Klebsiella and Flavobacterium species did not metabolize much of the substrate and showed no significant changes in the parameters mentioned. The drilling wastes were therefore concluded to have affected the soil microbial spectrum adversely but the affects waned very gradually with time and distance.
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