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Articles by R. Sharma
Total Records ( 7 ) for R. Sharma
  K.C. Das , R. Sharma , S.S. Paul , K. Dhama , N. Haque , K.K. Baruah and C. Rajkhowa
  Six fibre degrading fungus were isolated from rumen of mithun (Bos frontalis) at National Research Centre on Mithun, Jharnapani, Medziphema, Nagaland using specific media for rumen fungus. These isolated fungal cultures showed higher fibre degrading enzymes like CM cellulase, Xylanase and Avicelase activity. Addition of fungal culture in the mixed rumen liquor of mithun increased in vitro gas production. The identification and characterization of these isolates was done by DNA isolation, Polymerase Chain Reaction (PCR) amplification, sequencing and plotting of phylogenic tree. The result showed that two isolates were homologus to Orpinomyces species and four isolates were homologus to Neocallimastix species. These isolates can have potential to be used as microbial feed additives in ruminants.
  R. Sharma , R.C. Rajak and A.K. Pandey
  The edible ectomycorrhizal Basidiomycetes are difficult to inoculate in field for reforestation of trees and mushroom production due to insufficient mycelial colonization of substrate. Edible mushroom in the genera Cantharellus was tested for mycorrhization with Dendrocalamus using house waste tealeaves+sand based inoculum under laboratory and green house conditions using polythene bags and plastic boxes. Mycorrhizal seedlings were acclimatized in open pot soil. Dense Cantharellus mycelia colonized the substrate in 2-3 weeks. Inoculum survived for atleast six months and retained its viability. Occasionally few bacterial contaminants were observed, which were discarded. The cost effective method developed in present investigation can be used for tailoring large-scale seedling/nursery plantlets and sustainable reforestation of various tree species.
  S. Asthana , R. Sharma , N. Choudhary , P.A. Vyas , M.A. Rather and G. Krishna
  Cytogenetic characterization plays an important role in cataloguing and conservation of germplasm. Osteobrama belangeri is one of the important fish species in North East India and need urgent attention for conservation. Hence cytogenetic characterization was carried out for Osteobrama belangeri. Samples of the specimen were collected from Manipur, India and maintained in wet laboratory in live condition. Metaphase chromosome preparation was made using standard hypotonic solution, methanol acetic-acid fixation followed by Giemsa staining for karyotyping of the species. The diploid chromosome number was found to be 50 and the karyotype is composed of 14m+8sm+28 (st and t) with fundamental arm No. (FN) 72. Positive chromosome bands like C, G and NOR bands were developed on the metaphase chromosome spread. NOR bands were found on the constriction regions near centromeric, as well as, slightly away from terminal regions. While, G bands was at A, T-rich regions appearing to be away from centromere towards the end of the chromosome bodies. C bands were localized on the regions adjacent or immediate to the centromeres and on the constrictions as indicating the concurrency with constitutive hetrochromatin. The result showed the diploid chromosome number as 50 which is mostly seen in other cyprindis and the study could localize C, G and NOR bands for the first time on this species.
  N.S. Gill , R. Sharma , R. Arora and M. Bali
  The present study was carried out to evaluate the antioxidant and antibacterial activity of methanolic leaf extract of Hippophae rhamnoides L. (Sea buckthorn, SBT) leaves under in vitro conditions. The chemical composition of the methanolic leaf extract was quantified in terms of total phenol and flavonoid contents. The Hippophae rhamnoides methanolic leaf extract exhibited potent antioxidant activity determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and then by 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), assays. The scavenging capacity by DPPH and ABTS of the extracts was found to increase with every unit increase of Hippophae rhamnoides leaf extracts reaching 68% at 30 μg mL-1 concentration. Further, the extract was evaluated for antibacterial activity against three human pathogenic bacteria E. coli, A. protophormial and M. luteus, however, it was maximum against E. coli. The study shows that the methanolic extract of Hippophae rhamnoides leaves have marked antioxidant and antibacterial activities.
  S Negi , P Santisree , E. V Kharshiing and R. Sharma

Nitric oxide (NO) is involved in diverse plant growth processes; however, little is known about pathways regulating NO levels in plants. In this study, we isolated a NO-overproducing mutant of tomato (Solanum lycopersicum) in which hyper-accumulation of NO, associated with increase in nitric oxide synthase (NOS)-like activity, caused diminished vegetative growth of plants and showed delayed flowering. The hyper-accumulation of NO caused drastic shortening of primary root (shr) in the seedlings, while the scavenging of NO restored root elongation in shr mutant. Inhibition of NOS-like activity reduced NO levels and stimulated root elongation in the shr mutant seedlings, while inhibition of nitrate reductase (NR) activity could not rescue shr phenotype. The stimulation of NO levels in shr mutant also conferred increased resistance to pathogen Pseudomonas syringae. Application of pharmacological inhibitors regulating ubiquitin–proteasome pathway reduced NO levels and NOS-like activity and stimulated shr root elongation. Our data indicate that a signaling pathway involving regulated protein degradation likely regulates NO synthesis in tomato.

  Mahender Kumar , R.K. Agnihotri , R. Vamil and R. Sharma
  Coriander commonly known as Dhania or Chinese parsley is generally grown for its use in soups, salads, dressing vegetables, seasoning and chutney. Effect of two phytohormones viz. GA3 and 2,4-D on seed germination, seedling growth and various physiological and biochemical parameters were studied. The hormones were applied individually in different concentrations (10, 50 and 100 μM concentrations). Both the hormones enhanced the germination percentage, seedling growth (root and shoot length), leaf area, chlorophyll and carotenoid content. The application of these hormones also decreased the germination time. Maximum germination, shoot length, leaf area and carotenoid content was observed in 100 μm concentration of GA3. Root length, chl. a and chl. b was maximum in 50 μM of 2,4-D and 100 μm GA3, respectively. The application of two hormones exhibited a marked increase on all the parameters studied as compared to the control.
  R. Sharma and R. Gupta
  Fifty proteolytic bacterial cultures were screened for their potential to degrade chicken feather. Eight strains which degraded chicken feather within 24 h were further evaluated for degradation of surrogate yeast prion protein, Sup35 NM. Prion degradation was studied by congo red binding assay and a strain of Pseudomonas aeruginosa KS-1 was selected for further evaluation as it exhibited maximal shift in absorbance of congo red from 0.268 to 0.926. Keratinase from this strain was purified using Q-sepharose chromatography with 60% recovery and a purification fold of 2.24. It was identified as a 33 kDa monomeric protein having a pH and temperature optima of 7 and 50°C, respectively. It was stable over a broad range of pH ranging from pH 2-11 and was highly thermostable with a t1/2 of >2 h at 80°C. It was a serine, thiol activated protease with two fold increase in activity in presence of β-mercaptoethanol. It also hydrolyzed a range of complex protein substrates including casein, feather, fibrin. Amidolytic activity on synthetic substrates revealed that it efficiently cleaved phenylalanine and arginine. It hydrolyzed insulin B-chain at cystein, glycine, histidine, phenylalanine and leucine as revealed by ESI-MS analysis.
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