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Articles by R. Ouzrout
Total Records ( 2 ) for R. Ouzrout
  Z. Dendani , M.A. Arcangioli , P. Bezille , R. Ouzrout and N. Laouabdia Sellami
  Thirty-two strains of Staphylococcus aureus have been collected in the setting of an epidemiological investigation in Rhone-Alps region in France (collaboration AFSSA-ENV Lyon) from samples of milk of districts of thirty-two cows affected by clinical mastitis, belonging to 17 herds, on one period of 5 months (January 2007 to May 2007). This set of withdrawals has been achieved in 4 veterinary clienteles situated in Rhone-Alps. Twenty five strains of Staphylococcus aureus were the object of identification phenotypic and a genetic characterization. Epidemiological scorers have been taken, the profiles of resistance to the antibiotics, the profiles of Pulsed Field Gel Electrophoresis (PFGE). The one has been chosen here because of its excellent power of discrimination. A sensitivity of 89% of the isolates to all tested antibiotics has been determined by the method of disk by diffusion on agar Mueller Hinton (MH) (Sanofi Diagnosis Pasteur or Bio-Rad). The frequencies of resistance to the Penicillin to the ampicillin to the oxacillin to the Cefquinome to the Cefalotin and to the Amoxicillin + AC clavulanique is respectively 20, 08, 08, 08, 08 and 04%, those to the trimethoprime more the sulfamethoxazole and the Sulfamethoxypyridazine are 08 and 24%. No resistance of the strains of Staphylococcus aureus to the oxytetracycline to the Doxycycline to the erythromycin to the Spiramycin to the Lincomycin to the Gentamicin and to the Enrofloxacine. The molecular typage by Pulsed-Field Gel Electrophoresis (PFGE), after digestion of the chromosomal DNA of the isolates with the SmaI endonuclease restriction, revealed 09 different genetic profils (A-I). The two main pulsotypes A and B represented 58% together and have been found in 64% of studied exploitations. So the strains of Staphylococcus aureus are in majority of the genotype A and B. They belonged to the predominant genotypes and could have a certain predilection to cause some mastitis among the dairy cow. In spite of the heterogeneity of the some, the results of the PFGE are in favor of a dissemination of a small number limited of predominant genotypes.
  R. Bouzid , N. Laouabdia Sellami , A. Benkhelil , A. Hocine , R. Ouzrout and K. Touati
  The serums coming from 450 dairy cows have been analyzed for the detection of the brucellosis, the prevalence observed of the infection is of 10-44%, the test of the Intradermotuberculination for the detection of the tuberculosis raised that the prevalence observed of the infection is of 8-22%, with a significant difference (p<0.05) between the individuals of 2 years and those aged of >4 years. All studied sanitary trouble were present during all year with a predominance of the mastitis especially in beginning of spring, where the prevalence attain 44-88% and or the troubles of reproduction bound have the stake bottom were the order of 14-88%, the respiratory trouble especially appeared in winter with a peak of 21-77%, the digestive trouble were the order of 11% in the spring whereas the parasitosis, the piroplasmosis reached their maximum in summer with 21-55%, the locomotive trouble were constantly present all along the year touch the same beasts practically, with a peak in estimated winter to 18-22%, what showed that the season has an effect on the emergence of these different trouble with a valor highly significant p<0.001.
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