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Articles by R. Nandakumar
Total Records ( 2 ) for R. Nandakumar
  S. Dinesh Kumar , P. Santhanam , T. Jayalakshmi , R. Nandakumar , S. Ananth , A. Shenbaga Devi and B. Balaji Prasath
  The interaction of phosphorus and nitrogen on their removal by microalgae can be of great concern during their role as bioremediant of waste water. Microalgae play an imperative role in nutrient and heavy metal removal in wastewater by their biosorption mechanisms. The study was attempted to optimize the pH and retention time for nutrient and heavy metal (zinc) removal from aqueous solutions using immobilized marine microalga Chlorella marina as beads and powder. The study inferred that pH 6 was found to optimum for removal of all nutrients except nitrite. However, for heavy metal removal, the pH 8 was found to be suitable for biosorbent studies. Further, the present experiment inferred that the 24 h incubation was enough for nutrients and zinc removal while using immobilized microalga C. marina beads.
  R. Nandakumar , S. Babu , G. Amutha , T. Raguchander and R. Samiyappan
  Phytotoxic metabolite produced by the five different isolates of Sarocladium oryzae, the rice sheath rot pathogen, was isolated from its in vitro culture filtrate to know the variability among isolates in terms of toxin production. The toxin was purified through solvent extraction and then by gel filtration on sephadex column. Carbohydrate and protein content of the toxin and the quantity of phytotoxin produced in planta were varied among isolates. Toxin from the five isolates also showed variation in its biological activity as detected in its ability to induce sheath rot symptoms on detached leaf sheath, greenhouse grown plants and to cause leakage of electrolytes from rice leaf sheath. The symptoms produced by the purified toxin were, symptoms similar to those of natural infection by the pathogen. The virulent isolates SO1 and SO2 produced more amount of toxin, more leakage of electrolytes and severe sheath rot symptoms. The in planta toxin production by different isolates was detected serologically, using the polyclonal antibody produced against SO1 toxin. Correlation between the variation in toxin production and the virulence of the pathogen was discussed.
 
 
 
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