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Articles by R. Bhakyaraj
Total Records ( 3 ) for R. Bhakyaraj
  N. Yogananth , R. Bhakyaraj , M. Syed Ali and R. Muthezhilan
  Background and Objective: Kaempferol is the important polyphenolic flavonoids that is used to the remedy for cancers, cardiovascular disease, neuron disorder, cholesterol and serve as antioxidant and anti-inflammatory. Materials and Methods: The present investigation was the comparative analysis of kaempferol from field grown plant parts and in vitro callus and also the enhancement of kaempferol by the influence of yeast elicitor were determined. In the present study, at the concentration of 2.0 mg L1 2,4–D+0.5 mg L1 KIN gave the maximum callus growth for both stem and leaf explants (3.47±0.50e and 3.56±0.44e, respectively). Results: The maximum content of kaempferol in three type of samples was observed in this order; yeast elicitor treated samples > in vitro raised callus samples > field grown samples. The highest amount of kaempferol was fount in 1 mL yeast elicitor treated with stem callus sample (0.986 mg g1 dw). Conclusion: Therefore, the present finding it was proved that the highest accumulation of kaempferol in elicitor treated in vitro callus is provides resource for preparation of drugs against various diseases by the pharmaceutical industries.
  R. Bhakyaraj and K. Singaravadive
  The cashew apple is a tropical fruit, which is being wasted as manure after picking the nut attached in it. Understanding the presence of bioactive compounds and minerals in pseudo fruit will help in developing technologies for value addition of cashew apple. In the present study variation in minerals and bioactive compounds of cashew apple juice was investigated. Minerals like Calcium (Ca), magnesium (Mg), copper (Cu), potassium (K), phosphorous (P), iron (Fe), sodium (Na), zinc (Zn) and selenium (Se) were analyzed by ICP-OES (inductively coupled plasma optical emission spectrophotometer) and bioactive compounds by GC-MS. The results showed that cashew apple is rich in minerals especially potassium (3337 ppm), phosphorous (440.2 ppm) and followed by magnesium (279.3 ppm). Other minerals like Iron, sodium, zinc and calcium also present in significant level. The bioactive compounds like 9,12-octadecadienoic acid (Z,Z)- and 9,12-octadecadienoyl chloride (Z,Z)- etc were also present in cashew apple. The results ensured that the biowaste cashew apple is a good source of various essential minerals and bioactive compounds which can be used to develop nutritious value added food and beverages.
  N. Yogananth , R. Bhakyaraj , A. Chanthuru and S. Palanivel
  A micropropagation protocol for the important medicinal plant species Dregea volubilis was developed using nodal explants by culturing on Murashige-Skoog (MS) media fortified with different concentration of KIN and BAP. The highest number of multiple shoots (9.36±0.67e) was noticed at the KIN level of 3.0 mg L-1 followed by 8.26±0.62e shoots in KIN 4.0 mg L-1. The in vitro raised micro shoots produced highest percentage of rooting (78%) in MS media augmented with 1.5 mg L-1 IBA. The hardened plantlets were acclimatized in the green house conditions and then reintroduced in the Herbal garden attached to the Centre in which 75% of the plants survived.
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