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Articles by R. Begum
Total Records ( 4 ) for R. Begum
  K.M. Nasiruddin , R. Begum and S. Yasmin
  The present study was conducted at the USDA Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University, Mymensingh during July 2000-July 2002 to investigate the effect of different concentrations of 2,4-D on callusing and PLB formation; BAP (0, 2, 4 and 6 mg l-1) and NAA (0, 0.5 and 1.0 mg l-1) on formation of Protocorm Like Bodies (PLBs) and plantlet regeneration from Dendrobium formosum leaf callus. PLB formation, shoot proliferation, root formation, leaf number, shoot length and root length were recorded. The fastest callusing (9.32 days) and the amount (0.73 g explant-1) was the greatest with 1 mg l-1 2,4-D at 20 DAE, also the number of PLB was the highest (10.52 explant-1) at 60 DAE. Time required for PLB formation was the longest (32.72 days) with 0.50 mg l-1 2,4-D. Plantlet height, root length and number of shoots, leaves and roots per plantlet showed significant variation due to BAP and NAA addition. BAP at 2.5 mg l-1 showed the best results in combination with 1 and 2 mg l-1 NAA.
  S. Yasmin , K.M. Nasiruddin , R. Begum and S.K. Talukder
  The experiment was conducted in the USDA Biotechnology Laboratory, Biotechnology Department, Bangladesh Agricultural University, Mymensingh to observe the effect of NAA and BAP on callus formation and regeneration from leaf and internodal segment explants. Five levels of each of NAA (0, 1.25, 2.5, 5 and 10 mg l-1) and BAP (0, 0.5, 1, 2 and 4 mg l-1) were applied to MS media for callus induction as well as plantlet regeneration. Twenty explants were cultured in each combination. Leaf showed better performance in callus induction and plantlet regeneration. The highest percentage of callus (95%) was induced with 2.5 mg l-1 NAA + 2 mg l-1 BAP and also minimum time (8.13 days) was required for callus induction in the same concentrations. The percentage of regeneration showed the highest value (80%) with 2.5 mg l-1 NAA + 2 mg l-1 BAP of all the combinations. It was also observed that callus derived from leaf produced plantlets in a shortest period of time (23.68 days) compared to that from stem (28.96 days).
  Abdullah-Al-Mahin , A.B.M. Sharifuzzaman , M.O. Faruk , M.A. Kader , J. Alam , R. Begum and Harun-Or-Rashid
  Due to huge demand of citric acid, attempts are taking to introduce its efficient production either by using low cost substrates or by improving the potency of the fermentation microorganisms. In this study, sugarcane bagasse extract was used for citric acid production using wild type Aspergillus niger CA16 and its radiation mutant 79/20 by submerged fermentation. Fermentation was carried out up to 15 days using 5, 10, 15 and 20% of sugarcane bagasse extract which contained 21.06, 32.60, 43.50 and 53.20 g L-1 sugar, respectively. The fermentation medium was supplemented with prescott salt. With the increasing concentration of baggase extract, total titratable acidity and citric acid production was increased. Moreover, radiation mutant A. niger 79/20 had higher citric acid production than A. niger CA16. Maximum amount of citric acid (12.81 g L-1) was produced in the 20% bagasse extract medium by A. niger 79/20, whereas, CA16 produced 10.25 g L-1 citric acid in the same fermentation medium. Maximum substrate uptake, growth yield co-efficient and productivity were also found higher in case of the strain 79/20. Thus, radiation mutation induced improved citric acid production in A. niger 79/20.
  M.A.H. Chowdhury , R. Begum , M.R. Kabir and H.M. Zakir
  Microbial respiration (CO2 evolution),inorganic SO4-S and available P were monitored in a S deficient soil amended with dhaincha, cowdung and rice straw @ 1 g per 50 g soil during 30 days incubation. A basal dose of 250 μg N, 200 μg P and 250 μg K g -1 soil was applied. Dhaincha showed the highest decomposition rate and rice straw showed the lowest rate, while that of cowdung was intermediate. After 30 days of incubation, 53, 17 and 39% C was decomposed from cowdung, rice straw and dhaincha, respectively. Decomposition was also found to be related to its nutrient status (C:N:S ratio). Considerable S mineralization was recorded in dhaincha and cowdung amended soil and attained the maximum at 15th day (13.8 μg g -1S) and 30 (9.8 μg g -1 S), respectively. During rice straw decomposition S was immobilized markedly. Application of plant and animal residues caused significant increase in P mineralization. Net mineralization was 95.5, 87.6 and 50.8 μg P g -1 soil in dhaincha, cowdung and rice straw amended soil, respectively.
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