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Articles by R. Alam
Total Records ( 5 ) for R. Alam
  R. Alam , M.Z. Karim , M. Fida Hasan , M.A. Hossain and A.B.M. Mafizur Rahman
  The study was conducted with 45 Sugarcane clones grown at zero-N and 120 kg N ha-1 levels in order to evaluate their possible BNF (biological nitrogen fixation) capability under field conditions. Field evaluation led to identification of some clones viz., I 153/94, Co 846, B 34-104 and Isd 28 that demonstrated considerable BNF capability. The yields of these clones at zero-N were 54.30, 61.48, 53.69 and 63.97 t ha-1 but at 120 kg N ha¯1 it was 59.72, 65.75, 57.15 and 70.59 t ha-1, respectively. The non BNF clones Isd 2/54 and Isd 18 showed poor yield at zero-N (31.38 and 28.17 t ha-1) while at 120 kg N ha-1 it was 54.61 and 61.58 t ha-1. The BNF capable clones performed almost equally well under both zero-N and 120kg N ha-1 as demonstrated through number of tiller (000 ha-1), number of millable cane (000 ha-1) and leaf nitrogen content at 120 and 200 days after plantation (DAP). Under aseptic culture condition, root extract and cane juice from the BNF- endowed clones showed the presence of gram negative bacterium that have been subjected to further studies.
  R. Baksha , R. Alam , M.Z. Karim , S.K. Paul , M.A. Hossain , M.A.S. Miah and A.B.M.M. Rahman
  Multiple shoots were obtained from shoot tip explant of sugar-cane (Saccharum officinarum) cultured on MS medium supplemented with BAP (0.5-2.0 mg l-1), Kn (0.1-0.5 mg l-1) and IBA (0.1-0.5 mg l-1). Roots were induced in in vitro regenerated shoots on half MS medium supplemented with 5.0 mg l-1 NAA, IBA and IAA. Plant regeneration from shoot tip was the highest on MS medium supplemented with BAP 2.0 mg l-1 and IBA 0.5 mg l-1. The reported experimental findings present a method of plant regeneration of sugar-cane variety Isd 28 through shoot tip culture. The plantlets were successfully transferred to soil and the percentage of survivability under ex vitro condition was 70.
  M.Z. Karim , M.N. Amin , M.A.K. Azad , F. Begum , M.M. Rahman , S. Ahmad and R. Alam
  Effects of sucrose, agar and pH on in vitro shoot multiplication of Chrysanthemum were studied. Nodal explant from the ex vitro grown plant was used as the test material. For optimum shoot induction and multiplication in MS medium containing BAP+ sucrose 30 gm l-1, agar 6 gm l-1 and pH 5.5-6.0 proved more effective. The media having 30 gm l-1 sucrose showed the highest percentage of explant responded to shoot proliferation and that was 100%. This sucrose concentration also showed the optimum result for number of usable shoots per culture, number of node shoot-1 and average length of shoots and the values were 5.4±0.6, 5.1±0.8 and 5.6±0.4 cm. The highest proliferation response of the explant was observed on MS medium having 6 gm l-1 of agar and the frequency was 100%. Among different level of pH, the highest percentage of explant showing proliferation was observed on the media adjusted to pH 5.5 and 6.0. The results presented here proved to be suitable for the in vitro shoot multiplication of Chrysanthemum morifolium.
  M.Z. Karim , R. Alam , R. Baksha , S.K. Paul , M.A. Hossain and A.B.M. Mafizur Rahman
  Leaf sheath were used as explants to induce callus on modified MS media supplemented with 2, 4-D as growth regulators. Different concentrations of BAP, IBA, NAA and IAA were used to regenerate shoots and a concentration of 1.0 mg/l BAP and 0.5mg/l IBA was found superior in the optimum production of multiple shoots. NAA (5mg/l) showed best performance in the production of roots. The plantlets were successfully transferred to soil with 70 percent survivability.
  M.Z. Karim , M.N. Amin , Asaduzzaman , S. Islam , Faruk Hossin and R. Alam
  An efficient protocol was developed for direct regeneration, multiplication and rooting under in vitro conditions of Chrysanthemum. The frequency of multiple shoot regeneration response was 95 and 91%, for nodal segments and shoot tips, respectively when cultured on the medium containing MS +1.0 mg l-1 BAP. Efficient rooting was achieved on half strength of MS+0.2 mg l-1 IBA. In vitro raised plantlets were transferred to potted soil and finally transferred to the field.
 
 
 
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