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Articles by R. Mohan Kumar
Total Records ( 2 ) for R. Mohan Kumar
  G.C. Minakshi , K. Vasanth , Tanupriya , K. Ilango , R. Mohan Kumar , Aruna Agrawal and G.P. Dubey
  Garcinia indica have been used for centuries with claim for its medicinal value by traditional practitioners to treat numerous diseases. The anti-oxidant activity of G. indica fruit which were assessed by determining the total phenolic content, evaluating the 2, 2-diphenyl-1-picrylhydrazyl, (DPPH) activity, superoxide dismutase (SOD) activity, as well as its ability to inhibit Nitric Oxide (NO) and also their effect on H2O2 induced Reactive Oxygen Species (ROS) generation in human monocytic (THP-1) cells was investigated by tracking intensity of a cell permeable fluorescent dye. In addition, High Performance Liquid Chromatography (HPLC) was used to quantify the bioactive constituent of G. indica fruit extract responsible for its biological activity. The fruit extract of G. indica had the highest amount of hydroxycitric acid (HCA) and phenolic content showed potent antioxidant activity. The IC50 values for DPPH, SOD and NO scavenging activities were 50.34, 37.08 and 34.15 μg mL–1, respectively. Garcinia indica fruit extract (200 μg mL–1) attenuated ≥60% of H2O2 mediated ROS generation in THP-1 cells. The above data provides evidence that the fruit extract of G. indica is rich in natural anti-oxidants and thus justify its use in folk medicine especially in the management of free radical-mediated disorders.
  K. Ilango , N. Kasthuri Bai , R. Mohan Kumar , K. Ananth Kumar , G.P. Dubey and Aruna Agrawal
  High altitude herbal medicines offers remedy for many diseases, particularly for which no medicine is available. As high altitude plants grow under stressful situations and exposed to high UV radiations, they are reported to have immense potential. Various scientific studies conducted on Hippophae species (Elaeagnaceae) during the last decade confirm its medicinal values. The present study was carried out with a view to lay down standards which could be useful to detect the authenticity of these medicinal plants, further the microscopical and physiochemical evaluation were studied to reveal the differences among Hippophae rhamnoides L. and Hippophae salicifolia D. Don. For the microscopic observation, free hand cross sections of the leaves of Hippophae rhamnoides and Hippophae salicifolia were stained with phloroglucinol and hydrochloric acid (1:1) and studied according to standard methods. Physiochemical analysis was carried out as per WHO guidelines on quality control methods for medicinal plants. The cross section of both the species of leaves were almost similar and revealed that the lower epidermal cells were completely covered with apressed stellate trichomes and abundant sunken stomata. Ash values of Hippophae rhamnoides leaves, showed higher ash content, compared to Hippophae salicifolia. The Pharmacognostic and phytochemical profile of Hippophae rhamnoides and Hippophae salicifolia are highly dependent on environmental adaptability of the plants. The present study helps in identification and differentiation of both the species of Hippophae.
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