Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by R. Karthik Raja
Total Records ( 1 ) for R. Karthik Raja
  N. Ramesh , V. Rajesh Kannan , K. Karthikeyan , K. Nanthakumar and R. Karthik Raja
  Isolation of Bluetongue virus from blood samples of sheep and goat was carried out in the present study. Out of one fifty blood samples screened for seroprevalance of BTV antibodies by Agarose Gel Precipitation Test (AGPT) 42 gave positive results. The overall percentage of virus isolation was 28% from Embryonated Chicken Eggs (ECE). The identities of the isolates were confirmed by cytopathogenicity. All the isolates were passaged twenty one times in embryonated chicken eggs and further passaged in BHK-21 cell lines. The viral isolates adapted well to the cell culture system and produced cytopathic change like grouping of cells, polycaryon, syncytica formation, acidophilic and intracytoplasmic inclusion bodies in BHK-21 cells. This study confirms the BTV incidence in the tested blood sample with a possible means showing that the virus can easily adapt to ECE and BHK-21 cell line.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility