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Articles by R. Heidary
Total Records ( 4 ) for R. Heidary
  S. Tajdoost , T. Farboodnia and R. Heidary
  The aim of this study to show the importance of salt intracellular compartmentation as a tolerance mechanism by inhibition of Na+/H+ antiporter system. In this research 4 day/old Zea mays L. seedlings (var. single cross 704) were exposed to 200 and 300 mM NaCl with and without 100 and 200 micromolar amiloride. After 48 h, the roots and shoots of seedlings were harvested separately. The changes of total Na+absorption, the amount of malondialdehyde and the activity of antioxidant enzymes such as guaiacol peroxidase, ascorbate peroxidase and catalase were analysed. The results indicated that Na+ absorption has been increased by salt stress but was not influenced by amiloride. Malondialdehyde content and the activity of antioxidant enzymes such as guaiacol peroxidase, ascorbate peroxidase and catalase were increased in salt stressed plants specially in plants treated with salt and amiloride. Therefore salt stress has caused osmotic and oxidative stress in plants and amiloride as inhibitor of vacuolar Na+/H+ antiporter has been increased salt stress. Therefore we concluded that Na+ compartmentation in the cell is very important to reduce its damage in the cytosol and vacuolar Na+/H+ antiporter has an essential role in Na+ homeostasis in the cell by exporting excess Na+ to the vacuole.
  G.H. Laleh , H. Frydoonfar , R. Heidary , R. Jameei and S. Zare
  The anthocyanin pigment was extracted from the four different Berberis plant (B. khorasanica, B. integerrima, B. orthobotrys, and B. vulgaris) using the soaking and wetting in Ethanol (1% acidified). The extracted anthocyanin pigments then were exposed to number of environmental conditions, which could destabilize the anthocyanin molecules. These environmental conditions were included three different pHs (0, 1.5 and 3), various temperatures (5oC, 15oC, 25oC and 35oC) and presence or absence of light. The results of the study showed that increasing in pH, temperature or exposure to light is able to spoil the anthocyanin molecule. Another factor affecting the tolerance of anthocyanin towards the environmental condition is the role of different species. Among the various Berberis species anthocyanin pigment in B. khorasanica, showed the greatest resistance to destruction by environmental conditions followed by B. vulgaris, B. orthobotrys, and B. integerrima.
  L. Pourabdal , R. Heidary and T. Farboodnia
  Flooding stress has many important morphological and biochemical effects on plants. Because of the importance of determination the effects of flooding on plants and understanding of the tolerance mechanisms, in this research 4 day old zea mays L. (cv. single cross 704) seedlings were exposed to 4, 7 and 10 days flooding stress. At the end of each treatment the roots and shoots of seedlings were harvested separately. To show the histological effects of flooding on plants, cross section of roots and shoots were studied with light microscope. There was no clear change in the tissues structures of leaves and stems of different treatments in comparison with controls, but in the roots of plants the aeranchyma had been developed under stress condition especially in the mesocotyl region. The roots of flooded plants grow towards the soil surface despite positive geotropism of control roots. The chlorophyll a and b content and the ratio of chlorophylls a/b have been decreased but the amounts of soluble sugars have been increased in both the roots and shoots of seedlings. We conclude that flooding influences plants growth and life and development of aeranchyma and vegetative roots help plants to adapt itself to stress condition. So it is very important to know which plants are sensitive or tolerant and what are tolerance mechanisms in different plants to succeed in agricultural efforts.
  L. Pourabdal , R. Heidary and T. Farboodnia
  Flooding results in major changes in the soil environment. The slow diffusion rate of gases in water limits the oxygen supply; which affects aerobic root respiration as well as many (bio) geochemical processes in the soil. Production of Reactive Oxygen Species (ROS) and induction of oxidative stress in plant tissues are some of the other effects of flooding. Malondealdehyde (MDA) production is the indicator of lipid peroxidation reactions. A significant increase in MDA in plants which were flooded probably is the indication of fatty acid oxidation in the present of free radicals generated by flooding. In this research, seeds of zea mays were sown in pots filled with vermiculate and then the pots were transferred to a controlled growth room with photoperiod of 16/8h light/dark and temperature 27/23 day/night. After 24 h, the seedlings were flooded for 4, 7 and 10 days. After sampling MDA content as well as lipid peroxidation reaction, total protein content and activity including CAT, GPX and APX were measured, in those plants which were flooded total protein increased significantly. Flooded plants had a much higher MDA content than unflooded control plants. Antioxidant enzymes activity in flooded plants such as guaiacol peroxidase, ascorbate peroxidase and catalase increased.
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