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Articles by R. Baksha
Total Records ( 3 ) for R. Baksha
  R. Baksha , R. Alam , M.Z. Karim , Sk. A. Mannan , B.P. Podder and A.B.M.M. Rahman
  Microshoots were cultured on MS medium supplemented with different combinations of auxin, sucrose and pH level for in vitro rooting of callus induced sugarcane (Saccharum officinarum) variety namely Isd 31. Among the types and concentrations of auxin used the best rooting in all the microshoots was obtained with half strength (major salts) MS basal medium supplemented with NAA at 5.0 mg l‾1, sucrose at 50 g l‾1 and pH level 5.7 were the optimum conditions for rooting when microshoots were incubated 25+ 2°C under 16 h photo period regime. The regenerated plantlets were successfully transferred to soil and the percentage of survivability under ex vitro conditions was 75.
  R. Baksha , G.T. Mavlanov , G.B. Nasirova and S.A. Djataev
  The objective of this study was to determine the influence of different plant hormones on gossypol accumulation in callus culture and morphogenesis of cotton (G.hirsutum.L). Conditions have been described for in vitro culturing of tissues from the seeds of two different types of cotton (glanded and glandless). The callus cultures were initiated using defined levels of Kinetin (Kn) and 6-Benzylaminopurine (BAP) and 2.4-dichlorophenoxyacetic acid (2:4-D) and 1-naphthaleneacetic Acid (NAA) and were then transferred on the same media and on media with no hormones also. The gossypol content of each callus cultures and different parts (root, stem and leaf) of aseptically grown (7 to 10 days old) seedlings and seeds was determined using HPLC for cotton plants. The different levels of auxin and cytokinin in the culture media led to differentiation and increased the accumulation of gossypol.
  M.Z. Karim , R. Alam , R. Baksha , S.K. Paul , M.A. Hossain and A.B.M. Mafizur Rahman
  Leaf sheath were used as explants to induce callus on modified MS media supplemented with 2, 4-D as growth regulators. Different concentrations of BAP, IBA, NAA and IAA were used to regenerate shoots and a concentration of 1.0 mg/l BAP and 0.5mg/l IBA was found superior in the optimum production of multiple shoots. NAA (5mg/l) showed best performance in the production of roots. The plantlets were successfully transferred to soil with 70 percent survivability.
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