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Articles by R. Asmah
Total Records ( 5 ) for R. Asmah
  O. Fauziah , P. Hanachi , S. Yogespiriya and R. Asmah
  The effect of Strobilanthes crispus extract and glycyrrhizin in on rats induced with carcinoma is reported. The results showed significant, increase of liver microsome glutathione s-transferase (GST) in rats after 12 weeks. Treatment with glycyrrhizin caused decrease in liver GST activity compared to control. Treatment with Strobilanthes crispus extract caused overall decrease in liver GST activity almost close to control groups. The microscopic observation of the lesion score during hepatocarcinogenesis revealed that cells of cancer group without treatment were severely necrotic at week 12. However, cells of cancer group with S. crispus treatment appeared normal at the same period.
  P. Hanachi , S.H. Kua , R. Asmah , G. Motalleb and O. Fauziah
  Normal biochemical processes in human body may produce free radicals. These free radicals can, in turn, lead to oxidative stress related disease. This study examined the antioxidant activity of Berberis vulgaris fruit extract and its cytotoxic effect on human liver cancer cell line (HepG2).The antioxidant activity of Berberis vulgaris Fruit Extract (BFE) was assay by β-carotene bleaching and 1-diphenyl-2-picrylhydrazyl (DPPH) assay. The screening of cytotoxic effect was carried out by the microculture tetrazolium salt (MTT) assay on the human liver cancer cell line (HepG2). The BFE with concentration of 5-140 μg mL-1 was used. The control group cell was without any treatment. Intracellular Alkaline Phosphatase (ALP) activity is determined by p-nitrophenyl phosphate. The concentration of 5 μg mL-1 was chosen for this test. In β-carotene bleaching, ascorbic acid showed the mean total antioxidant activity of 96.16±5.09%, f ollowed by BHT (66.71±2.52) and BFE (59.91±8.64). In DPPH, the EC50 of ascorbic acid was 0.252±0.000 mg mL-1, BHT (0.612±0.009 mg mL-1) and BFE (0.685±0.033 mg mL-1). The IC50 of BFE was found 106.0±10.1 μg mL-1. Beside reduction in cell proliferation the crude extract was capable of enhacing the intracellular protein content in cell cancer line by one fourth while intracellular alkaline phosphatase activity increased by 7 fold. The results showed that processed commercial Berberis vulgaris exhibited antioxidant properties, has the ability of reducing cell viability and may had the potential of enhancing the ALP activity probably through structural changes.
  S. Yogespiriya , P. Hanachi , I. Patimah , R. Asmah and O. Fauziah
  This study was conducted to investigate the effectiveness of 5% (w/v) Strobilanthes crispus extract on rat liver during chemically induced hepatocarcinogenesis. Histological evaluation of liver was conducted to observe the cellular and morphological changes during hepatocarcinogenesis treated with Strobilanthes crispus. The histological evaluation revealed that a certain grade of inflammation or necrosis at portal and lobular region and stages of fibrosis during hepatocarcinogenesis was successfully reduced after administration of Strobilanthes crispus extract. However, these changes did not fully recovered by supplementation of Strobilanthes crispus to normal histological features of liver. This could be due to a short experimental duration and in addition, the supplementation of this extract to normal rat did not show any changes in normal hepatocytes. Thus, Strobilanthes crispus can be considered as a potential chemopreventive agent.
  G. Motalleb , P. Hanachi , S.H. Kua , O. Fauziah and R. Asmah
  This study was carried out to determine the total antioxidant activity and phenolic content of barberry (Berberis vulgaris) fruit extracts. The extract was prepared with distilled water and ethanol and methanol (80%), respectively. The -carotene bleaching and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay were used to determine antioxidant properties of barberry fruit by measuring the decrease in absorbance at 470 and 517 nm. In distilled water, Berberis vulgaris showed 82.52±0.64% free radical scavenging activity. In ethanol, Berberis vulgaris fruit extract showed 73.62±1.87% free radical scavenging activity, BHT 67.50±0.53% and vitamin C 88.56±0.43%, respectively. Berberis vulgaris fruit extract exhibited the higher free radical scavenging activity in distilled water with 82.52% and EC50 = 0.64 mg mL 1. The methanol 80% extract of Berberis vulgaris showed 60.15% -carotene bleaching assay and vitamin C 91.17%. Berberis vulgaris fruit extract in 80% methanol had the highest phenolic content followed by extract in water. The results of showed significant differences (p<0.05) in the means of free radical scavenging activities of barberry fruit in water and ethanol.

  J. Suherman , R. Asmah , O. Fauziah , I. Patimah and A. Nor Haslinda
  The administration effect of Strobilanthes crispus extracts (SC) during hepatocarcinogenesis in rats was studied to investigate the possible cancer suppressive effect of the component existed in the leaves. Hepatocarcinogenesis was induced using Diethylnitrosamine (DEN) (200 mg kg-1) and 2-acetylaminoflourence (AAF) (0.02% w/w). Glycyrrhizin (G), the commercial anticancer drug, was used for comparison. A total of 84 male Sprague-Dawley rats were divided into 14 groups viz control (N), DEN/AAF (C), SC1% (NS1), SC2.5% (NS2.5), SC5% (NS5), SC7.5% (NS7.5), DEN/AAF/SC1% (CS1), DEN/AAF/SC2.5% (CS2.5), DEN/AAF/SC5% (CS5), DEN/AAF/SC7.5% (CS7.5), DEN/AAF/G1% (CG1), DEN/AAF/G2.5% (CG2.5), DEN/AAF/G5% (CG5) and DEN/AAF/G7.5% (CG7.5). The effect of SC was investigated by identifying activities of liver and plasma γ-glutamyl transpeptidase (GGT) and alkaline phosphatase (ALP) and glutathione (GSH) concentrations. Treatment with DEN/AAF caused increase in all enzymes activities when compared to control. The administration of SC (1, 2.5, 5 and 7.5%) to the induced cancer rats decreased the microsomal GGT. T-test showed a significant difference (p<0.05) when the GGT level of all the treatment groups were compared to the control (N) and DEN/AAF (C). The findings suggest that supplementation of SC on DEN/AAF rats reduced the severity of hepatocarcinogenesis by reducing liver GGT and ALP activities and also the levels of GSH.
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