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Articles by R Albertini
Total Records ( 1 ) for R Albertini
  I Zegers , T Keller , W Schreiber , J Sheldon , R Albertini , S Blirup Jensen , M Johnson , S Trapmann , H Emons , G Merlini and H. Schimmel
  BACKGROUND:

The availability of a suitable matrix reference material is essential for standardization of the immunoassays used to measure serum proteins. The earlier serum protein reference material ERM-DA470 (previously called CRM470), certified in 1993, has led to a high degree of harmonization of the measurement results. A new serum protein material has now been prepared and its suitability in term of homogeneity and stability has been verified; after characterization, the material has been certified as ERM-DA470k/IFCC.

METHODS:

We characterized the candidate reference material for 14 proteins by applying a protocol that is considered to be a reference measurement procedure, by use of optimized immunoassays. ERM-DA470 was used as a calibrant.

RESULTS:

For 12 proteins [2 macroglobulin (A2M), 1 acid glycoprotein (orosomucoid, AAG), 1 antitrypsin (1-protease inhibitor, AAT), albumin (ALB), complement 3c (C3c), complement 4 (C4), haptoglobin (HPT), IgA, IgG, IgM, transferrin (TRF), and transthyretin (TTR)], the results allowed assignment of certified values in ERM-DA470k/IFCC. For CRP, we observed a bias between the lyophilized and liquid frozen materials, and for CER, the distribution of values was too broad. Therefore, these 2 proteins were not certified in the ERM-DA470k/IFCC. Different value transfer procedures were tested (open and closed procedures) and found to provide equivalent results.

CONCLUSIONS:

A new serum protein reference material has been produced, and values have been successfully assigned for 12 proteins.

 
 
 
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