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Articles by Qingzhong Xue
Total Records ( 2 ) for Qingzhong Xue
  Shaukat Ali , Zhang Xianyin , Qingzhong Xue , M. Jaffer Hassan and Haifeng Qian
  In this study, we established an efficient Agrobacterium-mediated transgenic system in rice (Oryza sativa L.). During the studies in the japonica (Xiushui-11) and indica rice cultivars (XC-95), the role of various factors responsible for virulence of Agrobacterium was screened more carefully to establish a better transformation system for the rice cultivars. MS and AB media (as VIM) proved to be efficient for Xiushui-11 and XC95, respectively. 100 μM AS (acetosyringone), pH 6.0 (VIM and COM) and use of either sucrose, glucose (Xiushui-11) or sucrose (XC-95) produced the best results for both of japonica and indica rice. Bacterial cell density A600 0.4-0.6 for 10-15 min was the most favorable optimized condition. Completely dark (Xiuishui-11) and light (XC-95) conditions during co-cultivation period were found to be ideal for transformation process. The significance of achieved results has been discussed properly.
  Haifeng Qian , Xianyin Zhang and Qingzhong Xue
  In the current study, the effect of different concentrations of 6-benzyladenine, the effect of light on callus induction, browning and GUS transient expression in Indica rice Pei’ai64s was investigated. The results indicated that embryos callus from seeds and GUS transient expression is favored by the addition of concentration of 6-benzyladenine to cultured medium and culturing under light condition. High 6-benzyladenine concentration(0.5, 1 mg L-1 )reduces the frequency of callus, GUS transient expression and enhances browning ratio. Light was also observed as browning for promoting factor
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