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Articles by Qing Zhu
Total Records ( 14 ) for Qing Zhu
  Juan Li , Cen Wang , Fan-Li Kong , Yan Wang , Xiao-Ling Zhao , Yi-Ping Liu and Qing Zhu
  MUSTN1 (Mustang, Musculoskeletal Temporally Activated Novel-1) gene is a musculoskeletal temporally activated novel gene which plays a key role in regulating the muscle development. In this study, the Single Nucleotide Polymorphisms (SNPs) in MUSTN1 were scanned, the association of SNPs with carcass traits in chickens were analyzed by Polymerase Chain Reaction-single-strand Conformation Polymorphism (PCR-SSCP) and DNA sequencing methods. Two variations (2120T>C and g.2160A>G) were identified when screened its entire exons and partial introns in 638 chickens. The genotypes of g.2120T>C (Mutations in exon 2) were significantly associated with breast muscle weight, muscle fiber diameters (p<0.05). The genotypes of g.2160A>G (Mutations in exon 2) were significantly associated with breast muscle weight, breast muscle weight percentage (p<0.05) and great significantly associated with muscle fiber diameters (p<0.01). MUSTN1 may act as a candidate gene of quantitative trait loci in regulating muscle growth.
  Ling-Bin Liu , Di-Yan Li , Xiao-Ling Zhao , Yi-Ping Liu , Yan Wang and Qing Zhu
  Prolactin Receptor (PRLR), as an important regulatory gene about growth and differentiation, might be a candidate gene for reproductive traits. The purpose in the present study was to analyze the association of Single Nucleotide Polymorphisms (SNPs) of exon 6 in prolactin receptor gene with egg production traits in Erlang mountainous chickens, a native breed from Poultry farm of Sichuan Agricultural University which consisted of two pure lines SD02 and SD03. Polymerase Chain Reaction-single-strand Conformation Polymorphism (PCR-SSCP) and DNA sequencing methods were used. Results showed that One SNP (G1836C) which had not yet been reported was found and two alleles (G, C) and two genotypes (GG, CC) were also detected. It observed significant differences in allelic and genotypic frequencies among all the chicken populations. Genotype frequencies in all chicken for GG and CC were 56.22 and 43.78%, respectively. Associations of polymorphisms of the PRLR gene with egg production traits were analyzed by using a GLM procedure. The CC genotype had extremely significant effect on Age at First Egg (AFE) (p<0.01) and the AFE of CC genotype had 3.75 days earlier than GG genotype. There was no significant association of G1836C with BWFE; body weight at first egg, WFE; weight at first egg, EN; egg number during 300 days after hatch, BWTA; body weight at 300 days of age and WTE; egg weight at 300 days of age. Therefore, the study showed that the PRLR gene may either a major gene that influences age at first egg of chicken or a molecular marker in close linkage with such a gene.
  Qingzan Kong , Qing Zhu and Liqi Wang
  Statins inhibit cholesterol synthesis by blocking 3-hydroxy-3-methylglutaryl coenzyme A reductase in the liver, thereby ameliorating hypercholesterolemia. Thus, to determine statins with the best efficacy, a meta-analysis was performed to compare the effects of statins against hypercholesterolemia. Comprehensive literature searches were established, from Cochrane library, Pubmed, Embase. The studies were performed to randomize controlled trials (RCTs), cohort studies or case-control studies about efficacy of different statin drugs and dose against hypercholesterolemia published between 1997 and 20 February, 2017. Study qualities were assessed according to Cochrane collaboration recommendations. The non-programming software Aggregate Data Drug Information System (ADDIS) (version 1.16.5) was used to perform Bayesian network meta-analysis and compare treatments using the Markov Chain Monte Carlo (MCMC) method. Overall, 28 RCTs studies, including 12855 patients, met the inclusion criteria. Total cholesterol (TC) levels significantly reduced (p<0.05) using 2 mg Pitavastatin (Pit) than those using 20 mg Pravastatin (Pra), 10 mg Simvastatin (Sim) or 10 mg Atorvastatin (Ato). Similarly, triglyceride (TG) levels reduced using 2 mg Pit than those using 20 mg Pra (p<0.05), 10 mg Sim (p<0.05) or 20 mg Sim (p<0.05) and reduced apolipoprotein B (Apo B) levels were observed than those using 10 mg Ato or 20 mg Pra (p<0.05). Rosuvastatin (Ros) significantly reduced TC and TG levels (p<0.05) when administered at 20 and 10 mg Ros treatments ameliorated percentage changes in low-density lipoprotein cholesterol more than the other drugs (p<0.05) and increased high-density lipoprotein cholesterol levels more effectively than 10 mg Ato (p<0.05), 20 mg Pra (p<0.05) or 10 mg Sim (p<0.05). Increases in Apo A1 levels did not differ between treatments (p>0.05). Among the present statin drug regimens, 2 mg Pit and 10 or 20 mg Ros had the highest efficacy against hypercholesterolemia.
  Huadong Yin , Zhichao Zhang , Xi Lan , Xiaoling Zhao , Yan Wang and Qing Zhu
  The MyoD gene family has been proposed to profoundly modulate muscle development and carcass performance in farm animals. In this study, researchers examined Single Nucleotide Polymorphisms (SNPs) in the exons of the Myf5, Myf6 and MyoG genes using Polymerase Chain Reaction (PCR)-Single Strand Conformation Polymorphisms (SSCP) and DNA sequencing methods in 360 individuals from 6 commercial pure lines of Sichuan Daheng meat type quality chickens. About 2 SNPs (87T>C and 96C>T) in exon 1 of Myf5, 1 SNP (154T>C) in exon 1 of MyoG and no variation in Myf6 were detected. The 96C>T SNP in Myf5 was a rare variant and was not analyzed further. The association analysis of genotypes with carcass traits revealed that the genotypes of SNP (87T>C) in Myf5 were significantly associated with Live Weight (LW), Carcass Weight (CW), Semi-Eviscerated Weight (SEW) and Eviscerated Weight (EW) (p<0.05). The SNP genotypes (154T>C) in MyoG were significantly associated with Live Weight (LW), Carcass Weight (CW), Eviscerated Weight (EW) and Breast Muscle Weight (BMW) (p<0.05). The results suggested that Myf5 and MyoG genes are potential major genes or are in close linkage disequilibrium with the QTL affecting carcass traits in this population of chickens. The 2 SNPs may potentially have use as markers for Marker-Assisted Selection (MAS) in chicken breeding.
  Yao-Dong Hu , Zeng-Rong Zhang and Qing Zhu
  Calpastatin (CAST) is a naturally occurring protein that inhibits normal tenderization of meat as it ages postmortem. The aim of this study was to investigate the effect of CAST gene polymorphisms on chicken carcass traits. The researchers screened CAST Single Nucleotide Polymorphisms (SNP) in 359 meat type quality chickens from 5 commercial pure lines (S01, S02, S03, S05 and D99; developed from Chinese local breeds), 3 crossbreeds (S01xS05, S01xS10 and S01xD99) and 4 native breeds from Guangdong (Fengkai Xinghua chicken, Huiyang Huxu chicken, Qingyuan Ma chicken) and Guangxi province (Xiayan chicken) in China. Three SNPs (36127T>C, 37752A>T and 37868G>A) were detected by Single Strand Conformation Polymorphism (SSCP) method and DNA sequencing. The linkage disequilibrium analyze found that only 37868G>A SNP in Hardy-Weinberg equilibrium yet one SNP can not compose haplotype, therefore these three SNPs should be analyzed separately rather than as haplotypes. Association analysis showed that the 37752A>T genotypes were significantly associated with Body Weight (BW), Carcass Weight (CW), Breast Muscle Weight (BMW) and Leg Muscle Weight (LMW). The results suggest that CAST SNP is significantly associated with carcass trait in the twelve studied populations and could be useful in selection for changing meat quality in chicken. Further investigations on more chicken populations with larger sample size are needed to confirm this conclusion.
  Huadong Yin , Yan Wang , Xiaoling Zhao , Shiyi Chen , Zhichao Zhang , Yiping Liu and Qing Zhu
  Fatty deposition in organism is an important factor which influences meat quality. Previously, Heart Fatty Acid-Binding Protein (H-FABP) gene was studied as a candidate gene which having effect on Intramuscular Fat content (IMF). In current study, we chose four SNPs loci (g.260T>C, g.675G>A, g.783C>T and g.1198T>C) of H-FABP which have been proved to have distinct correlation with IMF as molecular markers to scan SNPs in a new breeding Chinese yellow-feather broiler population. Researchers allocated adaptive individuals into high-IMF group and low-IMF group according to the haplotypes which were constructed of genotypes at four SNP sites of each individual in F1 generation and these haplotypes had been proved to be the preponderant or recessive haplotype. The core group was chose from the F1 generation based on the traditional breeding method. And then, we detected the genotypes of the four loci by SNPs screening and analyzed the selection effect in F2 generation which were obtained from mating and crossing within each group. Results indicated that the preponderance alleles were changed at Locus 1, 3 and 4 in the three groups of F2 and there were no significant differences in IMF among the three groups (p>0.05). However, the IMF of the core group and the low fat group was higher than which of high fat group. The result of variance analysis showed that there were no significant difference in the effect of the different genotypes of the four SNPs on IMF (p>0.05). The four loci on the H-FABP gene in the research were not suitable as candidate markers to select IMF in this yellow-feather broiler strain.
  Yao-Dong Hu , Jie Wen , Mai-Qing Zheng , Ran-Ran Liu , Ji-Lan Chen , Qing Zhu and Gui-Ping Zhao
  Four generations of bi-directional selection for antibody response to challenge with SRBC on days 56 were completed in White Leghorn chickens. The anti-SRBC titer and other immune traits were measured in up-selected (H), down-selected (L) and Control (C) lines in each Generation (G0-G4) and reproductive traits were measured in G4. In the 4th generation, the difference in SRBC titers (measured on days 61) between the selected lines was 7.18 versus 6.10, a 17% increase in the H compared to the L lines. The heritability of SRBC titer was 0.26. The genetic correlations between anti-SRBC and IgG (0.87) and IgM (0.81) were high and those between SRBC titers and those against AI (0.56), ND (0.48) and the H/L ratio (0.58) were moderate. Immune and reproductive traits compared among the H, L and C lines in the final Generation (G4) showed that AI, ND and IgM were improved significantly (p<0.05) by selection for SRBC but the age at first lay was slightly delayed (0.48 days) in the H line. This study demonstrates that selecting for antibody response to SRBC improves other immune traits consistent with broad disease resistance of chickens.
  Hua-Li Wu , Hui-Ying Wang , Da-Qian He , Yi Liu , Shao-Ming Gong , Fan-Li Kong and Qing Zhu
  Peroxisome Proliferator-Activated Receptor γ (PPAR-γ) was a key regulator of proliferation and preadipocyte differentiation in mammals. The objective of this study was to investigate the effect of the PPAR-γ gene on slaughter traits of 170 individuals from Zhejiang White geese (ZW) and the ontogenetic expression pattern. PCR-SSCP technique was developed to analyze coding region of the PPAR-γ gene polymorphism. The results showed that two genotypes (AA and AG) which were the products of two alleles (A and G) were observed. Association analysis indicated that this SNP was significantly associated with percentage of goose leg muscle (p<0.05) and the allele A was the predominant allele. Quantitative real-time PCR (qRT-PCR) assay measured the PPAR-γ mRNA expression in ZW and Landes goose five tissues of different ages (P1, P14, P21, P28, P35, P42, P49, P56 and P63). The results showed that the PPAR-γ mRNA had the highest expression level in goose abdominal fat and subcutaneous fat followed by lung and intestine. The PPAR-γ mRNA levels exhibited a rise-decline change in fat tissues except for ZW subcutaneous fat. The values in fat tissues were higher than those of other tissues at the same goose breed (p<0.01). Besides, average values in ZW fat and liver tissues were higher than those of Landes goose totally. These results suggested that the PPAR-γ expressions were positively associated with goose fat development and had a breed-related tendency. The PPAR-γ gene polymorphism could be used in Marker Assistant Selection (MAS) as a genetic marker for goose slaughter traits.
  Yue Yin , Xi Lan , Kai Tian , Tian-Jie Li , Qing Zhu , Yi-Ping Liu , Xiao-Ling Zhao , Hua-Dong Yin , Yao-Dong Hu and Yan Wang
  Interferon-β (IFN-β) and immunoglobulin M (IgM), immunoglobulin G (IgG) are essential in anti-retrovirus progress. ALV-J is a kind of ubiquitous retrovirus in chicken. To investigate whether natural Avian Leukosis Virus strain J (ALV-J) infection has effect on the concentrations of serum IFN-β, IgM and IgG, two important time points after hatching, 3 and 18 weeks age which represent the early and late stages of ALV-J infection, respectively were decided to study. The results reveal that the serum IFN-β, IgM and IgG increase at the early stage of ALV-J infection while decrease at the late stage compared with the control chickens which indicate that ALV-J infection boosts the immune response and up-regulates IFN-β and antibody while long-term infection suppresses immune function and down-regulates IFN-β and antibody.
  Michela Brazzoli , Alessia Bianchi , Sara Filippini , Amy Weiner , Qing Zhu , Mariagrazia Pizza and Stefania Crotta
  Infection with hepatitis C virus (HCV) is still a major public health problem, and the events leading to hepatocyte infection are not yet fully understood. Combining confocal microscopy with biochemical analysis and studies of infection requirements using pharmacological inhibitors and small interfering RNAs, we show here that engagement of CD81 activates the Rho GTPase family members Rac, Rho, and Cdc42 and that the block of these signaling pathways drastically reduces HCV infectivity. Activation of Rho GTPases mediates actin-dependent relocalization of the HCV E2/CD81 complex to cell-cell contact areas where CD81 comes into contact with the tight-junction proteins occludin, ZO-1, and claudin-1, which was recently described as an HCV coreceptor. Finally, we show that CD81 engagement activates the Raf/MEK/ERK signaling cascade and that this pathway affects postentry events of the virus life cycle. In conclusion, we describe a range of cellular events that are manipulated by HCV to coordinate interactions with its multiple coreceptors and to establish productive infections and find that CD81 is a central regulator of these events.
  Tao Li , Shu-min Fei and Qing Zhu
  In this letter, the delay-dependent state estimation problem for recurrent neural networks with both time-varying and distributed time-varying delays is investigated. Through available output measurements, a delay-dependent criterion is established to estimate the neuron states such that the dynamics of the estimation error is globally exponentially stable. The derivative of a time-varying delay can take any value and the activation functions are assumed to be neither monotonic, nor differentiable, which are more general than the recently commonly used Lipschitz conditions. Finally, two illustrative examples are given to demonstrate the usefulness of the obtained condition.
  Hua-Dong Yin , Zhi-Xiang Kuang , Hui-Ying Wang , Qing Zhu and Da-Qian He
  According to the public declaration by commercial companies, the commercial fatty liver line of Landes geese is grey feather. However, about 0.3% varieties of pure white or white with grey spots feather phenotype were observed in our purebred stock. So, we tentatively put forward a doubt whether some alien genetic components coexist in this commercial line. In order to prove this proposition, the mtDNA D-loop sequence variation were analyzed among 115 domestic goose individuals from this commercial fatty liver line and Chinese indigenous breeds. Total 11 haplotypes were determined and all, which could be classified into Asian haplogroup and European haplogroup. Seven landes geese residing in the E1-E3 haplotypes were clustered into the European haplogroup together with the wild greylag geese, while the remaining 45 Landes geese were grouped into the Asian haplogroup, which was predominantly occupied by the Chinese geese haplotypes. Although, the frequency (45/52) would be overestimated, we also reasonably believed that the matrilineal components of Chinese geese coexist in this commercial fatty liver line of Landes geese. In addition, the extremely low genetic diversity has been retained in the 2 sampled populations of Chinese geese, especially, in Wanxi white geese population.
  Hua-Li Wu , Xiao-Lin Zhao , Fan-Li Kong , Bing Zhou , Yao-Dong Hu , Juan Luo , Hui-Ying Wang , Qing Zhu and Da-Qian He
  PLIN plays a central role in the regulation of adipocyte metabolism which drives triacylglycerol storage in adipocytes and requires to hormonally stimulated lipolysis by cellular lipases. However, there is no report about the molecular structure and the expression profile of PLIN in goose. In this study, we cloned the goose PLIN gene and predicted bioinformatics. Quantitative Real Time PCR (qRT-PCR) assays were developed for accurate measurement of PLIN mRNA levels in Zhejiang White goose (ZW) and Landes goose different tissues from different ages (0-9 weeks). The results showed that goose PLIN cDNA sequence encoded an open reading frame of 527 Amino Acids (AA). The molecular phylogenetic tree among species were constructed that the mammal grouped together, Bombyx mori became another branch while the goose and chicken became another branch. It was found that PLIN mRNA were highly expressed in adipose tissues and to a lesser extent in cardiac and skeletal muscle and the difference is extreme significant between the fat and other tissues (p<0.01) yet, no expression in liver. In addition, there was a significantly age-related and breed-related change in fat tissues (p<0.05) and PLIN mRNA expression in ZW fat higher than that of Landes goose. All these results showed that the expression of PLIN mRNA in adipose tissues exhibited specific developmental changes, age-related and breeding patterns. The patterns of PLIN gene suggested that it played an important role in geese fat development. What’s more, further study is needed to reconfirm its function in a large population and in other breeds with different genetics background.
  Yao Dong Hu , Y. Zhou , Xin Hua Liu and Qing Zhu
  In this study, we investigated the correlations between microsatellite DNA polymorphic loci and growth and carcass traits in Chinese Silkies (Gallus gallus) to find possible candidate loci for marker-assisted selection. A total of 240 birds were scored for allele size at 18 microsatellite loci which were offspring of a dam line of Silkies specialized for production performances and a sire line of Silkies for growth performances. Totally, 123 alleles were detected from these polymorphic loci, with a range of 5-8 alleles and an average of 6.83 per locus. The frequencies of the 123 alleles ranged from 0.0532-0.4035. The average value of Polymorphism Information Content (PIC) was 0.805, ranging from 0.707-0.855. The average observed heterozygosity and the average expected heterozygosity of 18 markers was 0.244 and 0.833, respectively. Seven microsatellite loci were found to be correlated with seven growth traits and four carcass traits (p<0.01 or p<0.05). Allele 113 in MCW0254, allele 270 in LEI0146 and allele 172 in MCW0154 may be regarded as three candidate-loci for growth and carcass traits of Silkies.
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