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Articles by Qian Wang
Total Records ( 12 ) for Qian Wang
  Chao Dai , Yibao Ma , Zhenhuan Zhao , Ruiming Zhao , Qian Wang , Yingliang Wu , Zhijian Cao and Wenxin Li
  The misuse of antibiotics has led our age to a dangerous edge, as antibiotic-resistant pathogens appear to evolve more quickly than antibiotics are invented. Thus, new agents to treat bacterial infection are badly needed. Cationic host defense peptides are on the first line of a host defense system and are thought to be good candidates for treating bacterial infection. Here, a novel cationic host defense peptide, mucroporin, was cloned and characterized from the venom of Lychas mucronatus. The MIC for Staphylococcus aureus was 25 µg/ml, including antibiotic-resistant pathogens. Based on the molecular template of mucroporin, mucroporin-M1 was designed by amino acid substitution. The MIC for S. aureus was 5 µg/ml, including the antibiotic-resistant pathogens methicillin-resistant S. aureus, methicillin-resistant coagulase-negative Staphylococcus, penicillin-resistant S. aureus, and penicillin-resistant S. epidermidis. Moreover, mucroporin-M1 also inhibited gram-negative bacteria. The modes of action of mucroporin and mucroporin-M1 were both rapid killing by disrupting the cell membrane of bacteria, and the number of surviving bacteria was reduced by about 4 to 5 orders of magnitude immediately after peptide delivery. These results showed that mucroporin could be considered a potential anti-infective drug, especially for treating antibiotic-resistant pathogens.
  Fengru Xi , Xiaoting Liu , Qian Wang and Mengmeng Liang
  Cost stickiness is an important economic phenomenon and an important factor that affects the business performance. Based on the classical Change Model of Anderson et al. (2003), this study analyzes and examines the impact of equity nature and the opportunism incentives from the management on the cost stickiness of listed companies in China. One of the features of this study is that it analyzes the influence of opportunism incentives on cost stickiness from the perspective of equity nature. According to the research results, the phenomenon of cost stickiness exists in most of the listed companies and the existence of opportunism incentives intensifies the cost stickiness behavior. Meanwhile, compared with the non-state-owned listed companies, the cost stickiness behaviors that happened in the state-owned companies are more serious, and thus are more likely to be influenced by the opportunism incentives.
  Hongxin Yao and Qian Wang
  Using the panel data of 284 Chinese cities from 2002-2010, the study examines the effect of environmental regulation on exports. In order to measure the stringency of environmental regulation on trade development under the constraint of industrial advantage in different regions, the sample is divided into eastern, central, western, Yangtze River Delta and Pearl River Delta five groups respectively. Considering the endogeneity of the environmental regulation variable, the study adopts system GMM to estimate the model for robust results. It turns out that the environmental regulation exerts different impact on trade pattern owing to the difference in industry foundation, technology accumulation and factor endowment etc. On the national degree, the environmental regulation has a positive effect on the export trade. It also plays an important role in promoting the exports of central district as well as strengthening the trade competitiveness of western district, but its influence on the eastern district is not statistically significant. Furthermore, it is shown that the environmental regulation has insignificant effects on Yangtze River Delta and Pearl River Delta, since the capital-intensive production patterns have been gradually formed in the two major economic zones.
  Xuefeng Wang , Qian Wang and Hui Tang
  This study explores the law of impact of taking G115 for a long time on the athletic ability through adopting the method of taking ginseng extract G115 continuously in order to provide a scientific basis for the rational application of traditional Chinese medicine to improve human exercise ability. Methods: This experiment takes the middle-and-long distance athletes as the research object. The athletes are divided into two groups during training, the group of taking medicine takes G115 and the control group takes placebo. Twelve minute run and related changes in physiological indicators of the group of taking medicine and the control group should be observed and measured after eight weeks. Results: Compared with the control group, hemoglobin and blood sugar of the group of taking medicine significantly increased (p<0.05). There is no significant difference (p<0.05) in activity of creatine kinase, the anaerobic threshold and anaerobic threshold intensity and 12 min run between the group of taking medicine and the control group. Conclusion: Taking ginseng G115 during the training can significantly improve content of hemoglobin and blood sugar of athletes and tends to lower activity of creatine kinase, to increase anaerobic threshold and anaerobic threshold intensity and to increase scores of 12 min race.
  Qindong Sun , Qian Wang and Hongli Qiao
  Aiming at the mobile short message (SMS) hot topic extraction, the text features and statistical regularity of SMS are analyzed in this study. The formal description of SMS hot topic is given and an algorithm of SMS hot topic extraction based on feature association analysis is proposed. According to the proposed algorithm, feature words of SMS can be clustered into different word bags by calculating the association degree of these feature words and the hot topic can be identified by means of word bags matching. Experiments results show that the proposed algorithm can detect the hot topic in the SMS messages effectively, which is useful to the analysis of SMS popular sentiments.
  Liang J. Liu , Ming Zhong , Qian Wang , Fengping Wang , Li X. Shen and Wei Li
  Background: Early change of cells in Alzheimer’s Disease (AD) occurs in hippocampal region. Investigation of hippocampal neuronal cells were provide useful information about this disease. Quercetin is a phytoestrogen, which has similar biological activity as estrogen. Since the mechanism of quercetin in prevention of AD development is still unknown, there is a need to investigate whether, quercetin can regulate hippocampal neuronal cells. Methodology and Results: Rat hippocampal neurons were isolated from one day old Sprague Dawley rats and treated with estrogen, quercetin and estrogen receptor antagonists. Cell viability was determined by MTT assay, neurite outgrowth was measured by fluorescent microsope and estrogen receptors were determine by Western blot. Quercetin functions like estrogen to increase hippocampal neuronal cell viability. It can also increase neurite outgrowth. Moreover, the effect of quercetin on hippocampal neuronal cells was mediated by estrogen receptor alpha instead of estrogen receptor beta. Furthermore, quercetin increased estrogen receptor alpha expression, but did not affect estrogen receptor beta expression. Conclusion: In conclusion, quercetin like estrogen can enhance hippocampal neuronal cell viability and promote neuritogenesis through estrogen receptor alpha.
  Qian Wang , Chunxiang Zhang , Youshe Ren , Wenbin Yue , Liguang Shi and Fulin Lei
  Selenoprotein P (Sepp1) is a protein of considerable intrigue due to its unusual composition and requirements for its biosynthesis. Most selenoproteins contained a single selenocysteine residue, the human, bovine and rodent selenoprotein P genes encode proteins contained 10±12 selenocysteines. In this study, a full-length cDNA was cloned and characterized from Taihang black goat testes. The full-length of Sepp1 (1511 bp) cDNA contained an open reading frame encoding 347 amino acids. Sequence analysis showed that the predicted Sepp1 amino acids shared high identities with that of other species. The order of Sepp1 mRNA expression levels was: liver>lung>spleen>kidney, heart, testes, caput epididymidis and corpus epididymidis.
  Wen Du , Qian Wang , Jia-Kun Wang and Jian-Xin Liu
  The catalytic activity of a xylanase retrieved from a Fosmid Library of rumen microbiota in Hu sheep was improved by directed evolution using error-prone PCR. After the first round of random mutagenesis, one (C12) was selected from 1450 clones. And E12 with the highest activity was acquired from about 2500 clones in the second round. The specific activity of purified mutant C12 and E12 was 69.67 and 118.50 U mg-1, 1.2 and 2.0 times as that of its parent enzyme HA2, respectively. The optimum temperature of Wild Type (WT) and mutants were 50°C while the mutants displayed lower thermostability than WT. The optimum pH for HA2, C12 and mutant E12 was 5.0, 5.0 and 6.0, respectively. Both WT and mutants retained about 85% of initial activity at pH range from 5.0-9.0. Sequencing analysis revealed that C12 was mutated at two amino acid positions (Q14H and K57R) and E12 was mutated at Q14H, V20A, M53I and K57R. Compared to HA2, the mutation of Q14H and K57R increased the affinity of xylanase to substrate (km was changed from 11.22-2.42 mg mL-1) decreased catalytic efficiency (kcat from 42.82-9.82 sec-1). Nevertheless, further mutation of V20A and M53I reversed the km to 45.14 mg mL-1 and kcat to 198.75 sec-1 which suggested that V20A and M53I probably contributed to the enhanced activity.
  Yuan Wang , Wei-Na Wu , Qian Wang and Zheng-Yin Yang
  Cu(II) complexes of three bis(pyrrol-2-yl-methyleneamine) ligands were synthesized and characterized by elemental analyses, mass spectra, and IR spectra. X-ray diffraction analysis shows that [CuL3]2 is a dinuclear complex with an extremely distorted square-planar geometry. Furthermore, the antioxidant activities of the compounds have been investigated. The electrochemical properties of the Cu(II) complexes have also been studied by cyclic voltammetry. The Cu(II) complexes show similar superoxide dismutase (SOD) activity compared with that of the native Cu, Zn-SOD.
  Qian Wang , Yun Wang , Changyong Liang , Jianhua Song and Xinwen Chen
  The HA2 protein of the Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus (HearNPV) is a WASP homology protein capable of nucleating branched actin filaments in the presence of the Arp2/3 complex in vitro. To determine the role of ha2 in the HearNPV life cycle, ha2 knockout and ha2 repair bacmids were constructed. Transfection and infection analysis demonstrated that the ha2 null bacmid was unable to produce infectious budded virus (BV), while the repair bacmid rescued the defect. In vitro analysis demonstrated that the WCA domain of HA2 accelerates Arp2/3-mediated actin assembly and is indispensable to the function of HA2. However, analysis of the repaired recombinant with a series of truncated ha2 mutants demonstrated that the WCA domain was essential but not enough to yield infectious virions, and a hydrophobic domain (H domain) consisting of amino acids (aa) 167 to 193 played a pivotal role in the production of BV. Subcellular localization analysis with enhanced green fluorescent protein fusions showed that the H domain functioned as a nuclear localization signal. In addition, deletion of the C terminus of the ha2 product, a phosphatidylinositol 4-kinase homolog, dramatically decreased the viral titer, while deletion of 128 aa from the N terminus did not affect HA2 function.
  Yun Wang , Qian Wang , Changyong Liang , Jianhua Song , Ni Li , Hui Shi and Xinwen Chen
  Autographa californica multiple nucleopolyhedrovirus (AcMNPV) BV/ODV-c42 (orf101; c42), which encodes a 41.5-kDa viral nucleocapsid protein with a putative nuclear localization signal (NLS) motif at the C terminus, is a highly conserved gene among members of the Baculoviridae family. C42 is demonstrated to be essential for AcMNPV propagation and can bind to nucleocapsid protein P78/83, a viral activator for the actin-related protein 2/3 (ARP2/3) complex to initiate nuclear actin polymerization, which is essential for viral nucleocapsid morphogenesis during AcMNPV infection. Here, we report the identification of a novel pathway through which c42 functions in nucleocapsid assembly. Cotransfection of Sf9 cells with c42 and p78/83 plasmids demonstrated that C42 was capable of recruiting P78/83 to the nuclei of uninfected cells and that the NLS motif of C42 was essential for this process. To validate this nuclear relocation mode in bacmid-transfected cells, a c42-disrupted bacmid (vAcc42ko-gfp) and rescued bacmids with wild-type c42 (vAcc42res-gfp) or with NLS coding sequence-mutated c42 (vAcc42nls-gfp) were prepared. By immuno-staining, P78/83 was found to be localized in the cytoplasm of either vAcc42ko-gfp- or vAcc42nls-gfp-transfected cells, whereas P78/83 was relocated to the nuclei of vAcc42res-gfp-transfected cells. Furthermore, F-actin-specific staining confirmed that there was no actin polymerization activity in the nuclei of either vAcc42ko-gfp- or vAcc42nls-gfp-transfected cells, which might be attributed to the absence of nuclear P78/83, an activator of the ARP2/3 complex to initiate nuclear actin polymerization. We therefore hypothesize a mode of action where C42 binds to P78/83 in the cytoplasm to form a protein complex and cotransports to the nucleus under the direction of the NLS motif in C42 during AcMNPV infection.
  Pauline B. McIntosh , Stephen R. Martin , Deborah J. Jackson , Jameela Khan , Erin R. Isaacson , Lesley Calder , Kenneth Raj , Heather M. Griffin , Qian Wang , Peter Laskey , John F. Eccleston and John Doorbar
  The abundant human papillomavirus (HPV) type 16 E4 protein exists as two distinct structural forms in differentiating epithelial cells. Monomeric full-length 16E1E4 contains a limited tertiary fold constrained by the N and C termini. N-terminal deletions facilitate the assembly of E1E4 into amyloid-like fibrils, which bind to thioflavin T. The C-terminal region is highly amyloidogenic, and its deletion abolishes amyloid staining and prevents E1E4 accumulation. Amyloid-imaging probes can detect 16E1E4 in biopsy material, as well as 18E1E4 and 33E1E4 in monolayer cells, indicating structural conservation. Our results suggest a role for fibril formation in facilitating the accumulation of E1E4 during HPV infection.
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