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Articles by Qi Zhou
Total Records ( 3 ) for Qi Zhou
  Gang Luo , Li Xie , Zhonghai Zou , Qi Zhou and Jing-Yuan Wang
  Fermentative hydrogen production from cassava stillage was conducted to investigate the influences of temperature (37 °C, 60 °C, 70 °C) and initial pH (4-10) in batch experiments. Although the seed sludge was mesophilic anaerobic sludge, maximum hydrogen yield (53.8 ml H2/gVS) was obtained under thermophilic condition (60 °C), 53.5% and 198% higher than the values under mesophilic (37 °C) and extreme-thermophilic (70 °C) conditions respectively. The difference was mainly due to the different VFA and ethanol distributions. Higher hydrogen production corresponded with higher ratios of butyrate/acetate and butyrate/propionate. Similar hydrogen yields of 66.3 and 67.8 ml H2/gVS were obtained at initial pH 5 and 6 respectively under thermophilic condition. The total amount of VFA and ethanol increased from 3536 to 7899 mg/l with the increase of initial pH from 4 to 10. Initial pH 6 was considered as the optimal pH due to its 19% higher total VFA and ethanol concentration than that of pH 5. Homoacetogenesis and methonogenesis were very dependent on the initial pH and temperature even when the inoculum was heat-pretreated. Moreover, a difference between measured and theoretical hydrogen was observed in this study, which could be attributed to homoacetogenesis, methanogenesis and the degradation of protein.
  Fei Li , Chaojie Zhang , Yan Qu , Jing Chen , Xiang Hu and Qi Zhou
  In order to help to elucidate the transport and fate of perfluorinated acids (PFAs) in the environment, a reliable and sensitive analytical method has been developed in present study for determination of short- and long-chain PFAs in various solid matrices. The method consisted of solvent extraction of PFAs from solid matrices using sonication, solid phase extraction (SPE) using weak anion exchange (WAX) cartridges, clean-up of SPE eluent with dispersive carbon sorbent and quantitation by high performance liquid chromatography-negative electrospray-tandem mass spectrometry (HPLC-negative ESI-MS/MS). The method detection limits (MDL) and quantitation limits (MQL), which were analyte- and sample-dependent, ranged from 0.02 to 0.06 ng g−1 and 0.10 to 0.90 ng g−1, respectively. The recoveries of all PFAs were generally good enough for quantitative analysis of these chemicals (57−115%), especially for short-chain (<C8, 80−115%) PFAs excluded in previous studies because methods were not available. The precisions of this method, represented by the percent relative standard deviation (RSD) of spiked measurements, were in a range of 1−19%. In addition, matrix effect did not affect analyte quantification in solid matrices in most cases, and the validated method was successfully applied to analyses of short- and long-chain PFAs in various solid matrices.
  Shufen Wang , Yi Shen , Xiaohua Yuan , Kai Chen , Xiangyu Guo , Yongchang Chen , Yuyu Niu , Jian Li , Ren-He Xu , Xiyun Yan , Qi Zhou and Weizhi Ji
  The pluripotency and self-renewal of embryonic stem cells (ESC) are regulated by a variety of cytokines/growth factors with some species differences. We reported previously that rabbit ESC (rESC) are more similar to primate ESC than to mouse ESC. However, the signaling pathways that regulate rESC self-renewal had not been identified. Here we show that inhibition of the transforming growth factor β (TGFβ), fibroblast growth factor (FGF), and canonical Wnt/β-catenin (Wnt) pathways results in enhanced differentiation of rESC accompanied by down-regulation of Smad2/3 phosphorylation and β-catenin expression and up-regulation of phosphorylation of Smad1 and β-catenin. These results imply that the TGFβ, FGF, and Wnt pathways are required for rESC self-renewal. Inhibition of the MAPK/ERK and PI3K/AKT pathways, which lie downstream of the FGF pathway, led to differentiation of rESC accompanied by down-regulation of phosphorylation of ERK1/2 or AKT, respectively. Long-term self-renewal of rESC could be achieved by adding a mixture of TGFβ ligands (activin A, Nodal, or TGFβ1) plus basic FGF (bFGF) and Noggin in the absence of serum and feeder cells. Our findings also suggest that there is a regulatory network consisting of the FGF, Wnt, and TGFβ pathways that controls rESC pluripotency and self-renewal. We conclude that bFGF controls the stem cell properties of rESC both directly and indirectly through TGFβ or other pathways, whereas the effect of Wnt on rESC might be mediated by the TGFβ pathway.
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