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by
QIAN Kun |
Total Records (
2 ) for
QIAN Kun |
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Jin Wenjie
,
Zheng Zhiming
,
Zhang Yongzhi
,
Wang Qianqian
,
Qin Aijian
,
Shao Hongxia
and
Qian Kun
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Two hundred and sixteen Avian Pathogenic E. coli (APEC)
isolates were used for the determination of drug susceptibility to β-lactam
and the detection of β-lactam drug resistance-related genes OXA-1,
OXA-5, OXA-31 using circular Disk Diffusion Method and PCR. Results
showed that drug sensitivity rates among isolates were 190/216, 191/216, 162/216
to cefotaxime, ceftriaxone and ampicillin/sulbactam, respecively. The amplified
gene fragments analysis of β-lactam drug resistance-related gene sequence
of isolates showed 97%, homology with reference sequences from the GenBank.
The OXA-1 and OXA-31 positive rates were only 7.4 and 10.6% but the OXA-5 positive
rate was 30.6%. Seventy five strains carried only one kind of resistance genes,
twelve strains carried two kinds of resistance genes and two strains carried
all the three kinds of resistance genes. The integrated results of resistance
gene detection and drug sensitivity test showed that the strains isolated in
APEC on the rate of drug resistance testing and three resistance gene detection
rate compared to the rate of aminoglycoside resistance and their drug resistance
gene and the detection rate is not high but the growing development of bacterial
resistance this is the alert source of bacteria in the prevention and treatment
of poultry should pay close attention to rational drug use. In this study, the
source for the understanding of avian bacteria β-lactam drug resistance
mechanisms as well as generate a broader range of further drug resistance genes
to provide a reference to epidemiological studies. |
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HUO Jin-long
,
HUO Hai-long
,
MIAO Yong-wang
,
LI Fu-quan
,
LIU Li-xian
,
WU Ge-min
,
OUYANG Yi-na
and
QIAN Kun
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The Dahe pig is one of the representative indigenous pig breeds in the middle-altitude region of southwest China, and has played an important role in the local meat production for a long time. In order to estimate the genetic variability in its population, as well as provide the guidance and basis of genetic background for its conservation and further utilization, 76 microsatellite loci situated on swine’s 19 chromosomes were analyzed for 60 individuals sampled from their natural population by employing PCR and polyacrylamide gel electrophoresis. A total of 347 alleles were detected and all the loci were polymorphic. The number of alleles varied from 3 to 10, with a mean allele and effective allele number of 4.57 and 3.50 at each locus respectively. The mean heterozygosity and polymorphism information content of 76 microsatellite loci were 0.696 2±0.071 6 and 0.644 1±0.091 4, respectively. The results indicated that the genetic diversity of the Dahe pig was rich, and the Dahe pig has a good selection potential for breeding. |
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