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| Articles
by
QIAN Jin-qiao |
Total Records (
2 ) for
QIAN Jin-qiao |
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HE Ying-ying
,
LIU Shu-bai
,
QIAN Jin-qiao
,
LEE Wen-hui
and
ZHANG Yun
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βγ-CAT is a naturally existing protein complex of non-lens βγ-crystallin
and trefoil factor, purified from Bombina maxima skin secretions. In HUVECs, βγ-CAT
can be rapidly endocytosed via intracellular vacuole formation and translocated
to the nucleus to regulate cell fuction. In this paper, we found that it contains
conserved Walker B motifs (IILYDEPS, residues 6-13) and Walker A motifs (GQSLSGKS,
residues 96-103) in the βγ-CAT α-subunit sequence. βγ-CAT
showed potential NTP-binding and weak GTPase/ATPase activities in vitro. Through
Western blotting analysis, we found that the α- and β-subunits of βγ-CAT
participated in a 150 kDa SDS-stable protein complex formation, which also contained
positive ubiquitination signals in the βγ-CAT treated HUVEC. Furthermore,under
confocal microscopy, the immunofluorescence signals of ubiquitin and βγ-CAT
subunits were co-localized in the vacuoles that were distributed in the cytoplasm
and nucleus. In addition, βγ-CAT could induce several tumor cell`s detachment
and apoptosis, and selectively kill tumor cells. These findings provide a clue
to understand the mechanism of βγ-CAT endocysis and nuclear transport,
and give an insight to investigate the possible occurrence of similar molecule’s
cellular functions and action mechanisms of non-lens βγ-crystallins
and trefoil factors in mammals. |
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LIU Shu-bai
,
HE Ying-ying
,
QIAN Jin-qiao
and
ZHANG Yun
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βγ-CAT is a naturally existing multifunctional protein complex of non-lens βγ-crystallin and trefoil factor from Bombina maxima skin secretions. In this paper, we first analysed the time-response curve of hemolysis and intracellular potassium efflux induced by βγ-CAT on human erythrocytes. After βγ-CAT (3 nmol/L) in human erythrocytes was treated at 37¡æ for 5 min, we found that about 93.31±5.89% (P<0.01) of the intracellular potassium ions leaked, whereas the percentage of hemolysis was only13.12±1.92 % (P<0.05). We employed electron microscopy to observe the erythrocytes’ morphological changes and found erythrocytes were swollen and some had globular structures occurring on the cell surfaces, and fast releasing hemoglobins. These results indicated that the hemolytic effect of βγ-CAT on human erythrocyte membranes was due to intracellular potassium ion¡¯s rapid efflux. These results demonstrate morphological proof in understanding the mechanism of βγ-CAT pore-forming effect on human erythrocytes. |
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