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Articles by Q. Yin
Total Records ( 2 ) for Q. Yin
  M.H. Sulaiman , C.A. Kudi , J.O. Hambolu , S.A. Ojo , I.M. Hussaini , M.M. Roebuck , A.R. Dodson , T.R. Helliwell , Q. Yin and S.P. Frostick
  Cell-cell attachment in the epithelial and endothelial cell sheets are held in place by tight junctions, adherens junctions and desmosomes. Tight junctions are made up of three integral membrane proteins, namely; claudins, occludin and junctional adhesion molecules. There is a wealth of evidence in the literature suggesting that tight junctions are primarily involved in the sealing of cellular sheets thereby regulating paracellular ion movements between sheets of epithelial cells. Thus, tight junction proteins constitute an essential part of this barrier in perineurial and endothelial cells particularly in peripheral nerves. Schwann cells in the peripheral nerves are isolated from the adjacent tissues by the perineurium which creates a diffusion barrier responsible for the maintenance of endoneurial stability. Some researchers have suggested that oncogenic Ras down regulates tight junction barrier functions thereby leading to the disruption of these barriers. Therefore, the loss of tight junction barriers function may enable growth factors to penetrate from the surrounding tissues to quiescent Schwann cells of the peripheral nerves that are capable of proliferation thus, ensuing neurofibroma formation. Here the researchers demonstrate the cellular expression of Zonula Occludens-1 (ZO-1) to the perineurial fibroblasts of the normal peripheral nerve and benign neurofibroma.
  M.H. Sulaiman , C.A. Kudi , J.O. Hambolu , S.A. Ojo , I.M. Hussaini , M.M. Roebuck , A.R. Dodson , T.R. Helliwell , Q. Yin and S.P. Frostick
  Neurofibromatosis type 1 (NF1)-Von Recklinghausen disease is the most frequent single-gene disorder that affects the nervous system. NF1 is inherited in an autosomal dominant manner with an estimated incidence of about 1 in 3000. The NF1 gene is mapped to chromosome 17, (17p21). Its product neurofibromin reduces cell proliferation. The apparent lack of a suitable in vitro model of human Neurofibromatosis type 1 (NF1) has directly limited the progress of research on its tumourigenesis and therapy. The problems of establishing pure NF1 culture include the control of fibroblasts proliferation. At the moment, efforts put in place in order to extend the life span of NF1 Schwann cells and to suppress the growth of fibroblasts has yielded poor results in neurofibroma. Tumour specimens from 17 patients were processed for cell culture and grown at 37°C with 5% CO2 and 100% humidity. Key modifications in limiting fibroblast proliferation included Dulbecco modified medium devoid of 10% foetal calf serum at the initial stage of the cell culture. Following 60% confluence, the unattached cells (Schwann cells) were preferentially detached. The presence of Schwann cells and the absence of fibroblast were confirmed through the staining with S-100 protein and vimentin primary antibodies. Light microscopy demonstrated the typical spindle-shaped cells. Thus, the researchers describe an easy and efficient method of obtaining Schwann cells from NF1 tissues. These pure cultures of Schwann cells are useful tools for the study of the pathogenesis of NF1 in vitro.
 
 
 
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