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Articles by Q. Xu
Total Records ( 2 ) for Q. Xu
  H.H. Musa , B.C. Li , G.H. Chen , T.P. Lanyasunya , Q. Xu and W.B. Bao
  Traditional karyotyping is invented in animal research for several decades depend on the analysis of characteristic banding patterns along the length of chromosome. In the present study chicken metaphase chromosomes were obtained by peripheral blood lymphocyte culture techniques, G-band patterns were obtained with trypsin and Giemsa, C-band patterns were treated with barium and the nuclear organizer regions (NORs) were identified by silver staining. All species studied presented a diploid number of 78 chromosomes, with 10 pairs of macro chromosomes including the sex chromosome and 29 pairs of micro chromosomes. G-band patterns were found quite different between breeds. The dark stained of C-band was observed on micro chromosome and W chromosome. Karyotype resemblance near coefficient was possible for breeds clustering. The position of centromers, relative length, arm ratio and the evolutionary distance of chicken breeds was estimated. The application of chromosome karyotype and banding techniques was used to study the origin, evolution and relationship of species, also used for gene location and sex determination. While, in the Medical field was used to identify genetic disease. The techniques was consider as a base for further molecular research, for example FISH.
  B.C. Li , F.Yu , Q. Xu , L.G. Ni , G.H. Chen , X.M. Cheng , H.H. Musa and T.Z. Liu
  Chicken embryos from stage 15 to stage 45 were studied by means of serial section and light microscopy in order to investigate the relationship between the spermatogonium and the testicular development in early chicken embryos. The results showed that the glycogen in the PGCs (primordial germ cells) cytoplasm reduced gradually at the stage 22-28 (3.5th-5th hatching day). On the stage 29 (6th hatching day), the gonad of the embryo appeared the feature of testis and the glycogen in the PGCs cytoplasm reduced further. On the stage 31 (7th hatching day), the differentiation of ovary or testis was obvious and the glycogen in the PGCs cytoplasm later disappeared. On the stage 34 (8th hatching day), the testicular cord had began to differentiate. The each cord had been solid tubule with the spermatogonium. On the stage 35-37 (9th-11th hatching day), the number of testicular cord had been increased following embryonic development. The spermatogoniums were monolayer located in cord. At this stage, fewer sustentacular cells had been differentiated, while difficult to distinguish. On the stage 38-40 (12th-14th hatching day), the seminiferous tubules had been formed typically, the number of spermatogonium increased as well as sustentacular cells and the interstitial cells in seminiferous distributed in groups. On the stage 40-45 (16th-19th hatching day), the testis on the right was a little bigger than that of the left, the number of spermatogonium increased obviously and appeared like clusters of grape in the middle of seminiferous. Lumina in seminiferous tubule had formed and spermatogonium aligned with different layers.
 
 
 
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