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Articles by Preekamol Klanrit
Total Records ( 2 ) for Preekamol Klanrit
  Lakkana Laopaiboon , Sunan Nuanpeng , Penjit Srinophakun , Preekamol Klanrit and Pattana Laopaiboon
  This research aims to select and evaluate the performance of three high-ethanol-producing strains of Saccharomyces cerevisiae (TISTR 5048, TISTR 5339 and NP 01) in very high gravity (VHG) ethanol fermentation. The maximum specific growth rates (μmax) of TISTR 5048 and NP 01 grown in yeast extract malt extract broth containing 150 g glucose L-1 were 0.49 and 0.46 h, respectively while μmax of TISTR 5339 could not be determined due to cell flocculation. The ethanol production by TISTR 5048 and NP 01 was further carried out in batch mode at 30 °C under normal gravity fermentation (240 g glucose L-1) and VHG fermentation (280 and 320 g glucose L-1) and the initial cell concentration was 1x108 cells mL-1. The results showed that TISTR 5048 cultured in 240 and 280 g glucose L-1 gave the maximum ethanol concentration (P) with the value of 99.58 g L-1, while NP 01 cultured in 280 and 320 g glucose L-1 gave the maximum P with the value of 104.68 g L-1. Ethanol productivities (Qp) of NP 01 were slightly higher than those of TISTR 5048 at all conditions tested.
  Haruthairat Kitwetcharoen , Sudarat Thanonkeo , Preekamol Klanrit and Pornthap Thanonkeo
  Background and Objective: Bioactive compound production has been facing many challenges including a long cultivation time, the instability and the impurity of the products from natural plants. This study aimed to produce bioactive compounds from K. parviflora by using plant cell culture system and determine their antioxidant potential. Materials and Methods: Callus was induced from different organs of the plant including the shoots, rhizomes and roots using Murashige and Skoog (MS) medium supplemented with various Plant Growth Regulators (PGRs). Fast-growing friable calli from all explants were selected and used to establish cell suspension cultures. The biomass production, the accumulation of total phenolics, total flavonoids and the antioxidant activity of the cell culture extracts were determined and compared with those from intact rhizomes of K. parviflora. Results: All cell suspension cultures of K. parviflora exhibited high biomass yields. Furthermore, the productivities of the phenolics and flavonoids from K. parviflora cell cultures were also significantly higher than those from the intact rhizomes. The antioxidant capacity of the cell culture extracts was found to be comparable with that detected in the intact rhizomes of this plant. Conclusion: Based on the high biomass yield, high bioactive compounds productivity and antioxidant capacity, K. parviflora cell culture system displayed great potential for commercial-scale production. Further investigations on the multiple biological properties of the bioactive compounds produced by K. parviflora cell suspension cultures as well as the cytotoxicity test will be explored.
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