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Articles by Prasong Srihanam
Total Records ( 7 ) for Prasong Srihanam
  Kittisak Kerdchan , Nuanchai Kotsaeng and Prasong Srihanam
  Background and Objective: Oxidative compounds are gradually interested and studied, especially agricultural sources for value-added in health and cosmetics. The objective of the present study was to screen the oxidative compounds of the sugarcane (Saccharum officinarum L.) planted in Maha Sarakham Province, Thailand. Materials and Methods: The four cultivars of sugarcane were extracted by ethanol before the screening of phytochemicals; total phenolic, flavonoid, triterpenoid, saponin and condensed-tannin. The extracts were then tested for antioxidation using different assays. Correlation between the phytochemical and antioxidation as well as the individual phenolic compounds were analyzed. Results: All tested phytochemicals found in the node extracts higher content than the rind extracts. The node extracts of all sugarcane cultivars have a higher potent activity than the rind extracts. The phenolic compounds and antioxidation activity were significantly different by cultivars and parts of the sugarcane. All phytochemicals have positively correlated to all tested methods with moderate to high values. The main substances were catechin, quercetin, ferulic acid, resveratrol, gallic acid and epicatechin respectively. Conclusion: The sugarcane is an important source of phytochemicals which expressed high antioxidant activity. It might be used the phytochemicals from the sugarcane for health and beauty applications.
  Phongsathorn Motham , Ansaya Thonpho and Prasong Srihanam
  Background and Objective: In the sugar production process, the sugarcane tree which squeezed sucrose already were called bagasse. They have been reported as agricultural sources for phytochemicals and would be applied in health supplement products. The objectives of this work were to fractionate the crude extracts of different sugarcane bagasse cultivars, Authong 17 (AU17) and Suphanburi 72 (SP72) using silica gel column chromatography and investigate their phytochemical contents and antioxidant activities in each fraction. Materials and Methods: The two cultivars of sugarcane bagasse were extracted by ethanol before fractionation by silica gel column chromatography. The fractions were collected and then investigated for phytochemicals and antioxidant activities. The individual types and contents of phytochemicals were analyzed by High-Performance Liquid Chromatography (HPLC). Results: The total phytochemicals including total phenolic, flavonoids, saponin, proanthocyanidins and condensed-tannin as well as individual phytochemical contents were varied by the fractions depending on the eluting solvents. Moreover, the antioxidant activity of the fractionated extracts was also varied following the eluting solvents. Conclusion: The bagasse is a naturally good source of phytochemicals with an antioxidant activity that would be developed from sugarcane bagasse extracts for further health benefit products.
  Prasong Srihanam
  This study was aimed to prepare silk fibroin (SF) of Thai silk (Bombyx mori) and cassava starch blend films by solvent evaporation method. The SF solution was firstly prepared from the cocoons by dissolving pure silk fibroin with tertiary solvent system of CaCl2: Ethanol: H2O (1:2:8 by mol). Cassava starch solution could be prepared by boiling at high temperature. The mixture of the SF and cassava starch solution was then blended and cast on the polystyrene plates. The obtained films were investigated on their morphology, secondary structures and thermal properties by using SEM, FTIR and thermogravimetry, respectively. The results found that SF blended with starch could be formed the higher ratio of β-sheet. With cross-section images, the blend films showed homogeneously texture. In addition, the blend film gradually increased its thermal properties when the starch blended ratio was increased. In conclusion, the SF/starch blend films could be adjusted their properties by varying the blend ratio.
  Prasong Srihanam
  This study aims to prepare silk fibroin film of Eri (Philosamia ricini) and investigate their conformational structure and thermal properties after treating with methyl alcohol. The Eri silk fibroin solution was obtained by dissolving the Eri cocoons with 9 M Ca(NO3)2. The silk fibroin hydrolysate was dialyzed to remove the concentrated salt against distilled water. It was then concentrated to give 2% silk fibroin by weight. Ten milliliter diluted silk fibroin to 0.5% weight per polystyrene plate was used to cast film at room temperature for 2 days. The obtained films were then treated with 80% methyl alcohol with different times. They were subjected to investigate using Fourier transform infrared spectroscopy (FTIR) and thermogravimetric analyzer for conformational and thermal studies, respectively. The FT-IR spectra showed that Eri silk films were composed of both α-helix and β-sheet structures before exposure to alcohol and changed from low content of β-sheet structure to higher ratio when immersed in alcohol. In addition, the β-sheet structure gradually increased according to the increase of treating time used. The relative data were obtained from thermal investigation since the decomposition temperatures of Eri silk fibroin films were increased as follow by the increase of methyl alcohol treating time. It is promising that methyl alcohol can be affected to change both conformation and thermal behavior of the Eri silk fibroin film.
  Yodthong Baimark and Prasong Srihanam
  Silk Fibroin (SF) microparticles containing hollow structure were prepared by a water-in-oil emulsion solvent diffusion method without any surfactants. Aqueous SF solution and ethyl acetate were used as water and oil phases, respectively. Influences of SF concentration and post methanol treatment on microparticle characteristics were investigated. All microparticles contained open hollow structures. Microparticle sizes increased with the SF concentration. Conformation of SF microparticles determined from FTIR spectra changed from predominantly random coil to β-sheet form after methanol treatment. Particle surfaces of methanol-treated SF microparticles were rougher than those of non-treated. The SF concentrations and methanol treatment did not affect open hollow structure of the SF microparticles. Major advantages of this method are the elimination of surfactants and extractable pore templates. These hollow SF microparticles are expected to be potential used as biodegradable microcarriers of cell and protein drug, because their hollow structures should permit cell attachment and enzyme immobilization.
  Prasong Srihanam
  The objective of this work was to study the effect of temperatures and incubation times on Silk Fibroin (SF)/Chitosan (CS) blend films properties. The films were firstly prepared by mixing the SF and CS solution in homogeneously blended, then cast on the polystyrene culture plates before taking into the oven at 40°C for 3 days. The obtained SF/CS blend films were treated at 90 and 120°C, each for 30, 60 and 90 min, respectively. All of films were characterized for their morphology and secondary structures by using SEM and FTIR, respectively. The results showed that SF/CS films have homogeneous texture without phase separation. However, they also appeared some particles dispersed and embedded on their surfaces. The surfaces of the films slightly increased smooth texture when increased both temperatures and incubation times. The results were also noted that the characteristics were varied by those different temperatures. FTIR spectra indicated that the absorption bands at amide regions (I, II and III) of the SF were similar profile. Moreover, treatment the blended films with temperatures resulted to changed the secondary structures of the films. This means the ratio of β-sheet were increased. It can be expected that water evaporation by increasing temperature resulted to make the molecules of SF and CS close up and help to form H-bonds between them.
  Kusavadee Sangdee , Aphidech Sangdee and Prasong Srihanam
  Background and Objective: Study on medicinal plant extract is gradually interested and distributed, especially their biological activities. The present study aimed to determine the enzyme inhibition and antimicrobial activities of the fractionated extracts of wild grape (Ampelocissus martinii Planch.) seeds. Materials and Methods: Wild grape seeds in different growth stages were extracted with methanol before fractionation by silica gel chromatography. The anti-glucosidase and anti-tyrosinase enzyme activities of the extracts were then tested by using UV-Vis spectrophotometry and antimicrobial activities were observed from MIC, MBC values and time killing assay. Results: The sub-fraction of immature stage eluted by ethyl acetate/methanol at 75/25 (%v/v) has the highest enzyme inhibition activity and the most potent efficiency for time kills profiles. The MIC values of the potent immature, mature and ripe fractioned extracts were ranging from 1.25-50.00, 1.25-50.00 and 1.56-25.00 mg mL1, respectively, while the MBC values ranged from 3.12-6.25, 3.12-25.00 and 3.12-25.00 mg mL1, respectively. Conclusion: The wild grape seed composed of α-glucosidase and tyrosinase inhibition and antibacterial activities compounds. The wild grape seed extracts may be used as active ingredients sources of health-supporting products or cosmetics.
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