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Articles by Prashant Kaushik
Total Records ( 6 ) for Prashant Kaushik
  Prashant Kaushik
  Background and Objectives: The modern-day eggplant consumers desire varieties with a higher content of chlorogenic acid, but the cultivated varieties of eggplant are with a lower content of chlorogenic acid. Whereas, the wild relatives of eggplant are higher in phenolic acids. Therefore, this study characterized the cultivated eggplant and its wild relatives for the fruit dry matter content, total fruit phenolics and chlorogenic acid content. Materials and Methods: Fruits of the accessions of cultivated eggplant, one primary genepool species, nine secondary genepool species and three tertiary genepool species were characterized for dry matter content (%), total phenolics and the fruit chlorogenic acid content (mg g1). The chlorogenic acid content in the fruit flesh was determined by using the High Performance Liquid Chromatography (HPLC). Results: Highest content of dry matter content of around 29% was determined for the species S. tomentosum and S. elaeagnifolium. Whereas, the highest content of total fruit phenolics were determined in the secondary genepool species S. linnaeanum. The most top content of chlorogenic acid around 4.5 mg g1 of fruit dry weight was present in the species S. linnaeanum and S. torvum. Different clustering approaches were able to cluster the primary genepool species with the cultivated eggplant. Conclusion: Overall, this work provides important information about the wild relatives of eggplant concerning their dry matter content, total phenolics and chlorogenic acid content. This information can be used to engineer eggplant varieties rich in fruit phenolics.

  Ishan Saini , Himanshi , Kavita Rani , Neha Gill , Kajal Sandhu , Neema Bisht , Tarun Kumar and Prashant Kaushik
  Microbes play a vital role in ecosystem stability. Here, microbes-Acacia association is discussed with particular reference to Arbuscular Mycorrhizal Fungi (AMF) which help in the establishment of crop-plants, especially in arid and semi-arid areas. The association helps to restore the structural composition of soil from the hazardous impact of agrochemicals, increase resistance against various pathogenic attack as well as several abiotic stresses. Further, a comparative account of microbes found in the rhizosphere of Acacia is illustrated. Among these, Rhizobia, Acetobacter, Bradyrhizobium, Bacillus, Pseudomonas and Trichoderma were described in detail. All these microbes can be regarded as Plant Growth Promoting Rhizospheric Microbes (PGPM), some of PGPM are Phosphate Solubilizing Microbe (PSM). Both of them help AMF for infecting mycorrhizal hyphae inside the plant cell. Overall, microbes can be used as biofertilizers along with other organic compounds, that can compensate for the nutrient's availability.
  Ishan Saini , Tarun Kumar , Jyoti Chauhan and Prashant Kaushik
  Members of genus Acacia comprises of trees used as fuelwood, timber and fodder. Moreover, some parts of plants are also used for their therapeutic properties. The development and applications of breeding and biotechnological tools are advancing at a significantly fast rate. Molecular markers and genomics offer vital information with regard to the inherited variation. The aim of this study to complied and discussed the developments in molecular maker technology, genomics and genetic engineering concerning genus Acacia. Overall, this information will be useful to gain awareness about the crucial trees in the genus Acacia from breeding and a biotechnological perspective.
  S. Vijeth , I. Sreelathakumary , C.S. Aiswarya and Prashant Kaushik
  Background and Objective: Chilli pepper is an important spice crop and viral diseases hamper the successful of chilli peppers. The present investigation entitled was carried out to identify the sources for Chilli leaf curl virus (ChiLCV) resistance. Screening of genotypes against the ChiLCV is crucial to select the appropriate genotypes to get the successful crop production under the disease pressure. Materials and Methods: A collection of 70 popular chilli genotypes across India were evaluated under the open field conditions and using artificial inoculum for the ChiLCV disease. Thereafter, the virus presence and absence was also determined by using the Polymerase Chain Reaction (PCR) using universal primers (AV494/AC1048). Results: It determined that 23 genotypes were moderately susceptible, 12 each were susceptible and moderately resistant, 10 were symptomless, 6 were resistant, 5 were highly resistant and 2 were highly susceptible. Further, the 10 symptomless and 5 highly resistant genotypes identified under open field conditions were subjected to artificial screening by using whitefly mediated and graft inoculations. Thereafter, the 6 resistant genotypes identified with artificial inoculation by showed the presence of the virus when confirmed with PCR. However, in the whitefly mediated inoculation, four genotypes viz., Sel-3 (T1), Sel-4 (T2), Sel-6 (T3) and CHIVAR-1 (T4) did not show any amplification for the presence of the virus. Conclusion: Overall, this study provides useful information regarding the behaviour of popular chilli cultivars/genotypes against the ChiLCV disease.
  Gurpreet Singh , Rambir Singh Kanwar , Lochan Sharma , Neeraj , Lakshman Kumar Chugh and Prashant Kaushik
  Background and Objective: Meloidogyne graminicola is a potential threat to the successful cultivation of the pearl millet, significantly reducing its yield and produce quality. Moreover, biochemical and physiological changes induced by Meloidogyne graminicola susceptible and resistant hybrids of pearl millet is not known in detail. The study was focused on the biochemical changes induced by the root-knot nematode,Meloidogyne graminicolaon pearl millet hybrids to determine the role of different biochemical on nature of resistance of the plants. Materials and Methods: Resistant (HHB 146) and susceptible (HHB 272) pearl millet hybrids were grown in steam-sterilized soil with two different sets, i.e., inoculated and uninoculated. Data was recorded on various biochemical parameters from roots and shoot portion on 15, 30 and 45 Days After Inoculation (DAI). Results: Total protein and phenols were increased in the roots and shoot portion of the infected plants. Total sugar was reduced inM. graminicolainfected plants and this reduction was more pronounced in case of susceptible inoculated plants. Phenol content increases as a result of nematode infection. On the other side, due to nematode infection sugar content in roots of susceptible hybrid is comparatively lesser as compared to resistant hybrid. Conclusion: It is concluded that root-knot nematodes,M. graminicolabring about significant biochemical changes in infected plants, which appear to employ physiological and biochemical strategies either to avoid or to tolerate the nematode infection.
  Hari Kesh and Prashant Kaushik
  Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is the major limiting factor in successful rice production. The disease causes around 20-30 % annual loss in rice production and under severe conditions, the yield loss goes upto 50%. The development of resistant genotypes against this disease is the most effective and economic way to control production loss rather than spraying harmful chemicals that affects the environment. In this direction, several varieties with the single resistant gene have been released for cultivation but due to continuous evolution of new pathotypes, there is a continuous breakdown of resistance against the bacterial blight disease. Although durable resistance can be attained by introducing multiple resistant genes in a single desirable genetic background. But with conventional breeding, it is challenging due to dominance and epistatic effects of disease resistance genes against bacterial blight. However, marker-assisted breeding made it possible to identify and introduce multiple genes into a desirable genetic background with rapid, recurrent parent genome recovery and with minimum linkage drag. Molecular markers play a significant role in speeding up the disease resistance breeding programs with different stages like screening, identification, mapping and cloning of disease-resistant genes y. Hereafter, in this review article the application and achievements of marker-assisted breeding in rice against bacterial blight disease was summarized.
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