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Articles by Prasetyastuti
Total Records ( 4 ) for Prasetyastuti
  Prasetyastuti , Sunarti , Ahmad Hamim Sadewa , Sri Mursiti and Mustofa
  Background and Objective: Type 2 diabetes is characterized by hyperglycemia leads to increased production of reactive oxygen species. There is increasing evidence that active compounds of medicinal plants may be used to reduce oxidative stress. The aims of this study were to investigate anti-diabetic effects of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one derived from Swietenia macrophylla King seed on oxidized LDL, homeostatic model assessment beta-cell function (HOMA-β) and glucagon like peptide 1 (GLP-1) gene expression in diabetic rats. Materials and Methods: A total of 30 rats were used. They were divided into 6 groups as follows: (A) Normal rats, (B) Diabetic rats, (C) Diabetic rats with metformin, (D), (E) and (F) Diabetic rats with 7-Hydroxy-2-(4-hydroxy-3-methoxy-phenyl)-chromen-4-one Swietenia macrophylla King seed dose 10, 30 or 90 mg/200 g b.wt., respectively. Oxidized LDL and HOMA-β value were analyzed twice, before and after treatment. The GLP-1 gene expression in intestine was analyzed at the end of study. Data were analyzed by paired t-test and one-way ANOVA followed by Games-Howell test. Results: Administration of three different doses of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one from Swietenia macrophylla King seeds in diabetic rats significantly reduced oxidized LDL and increased HOMA-β value (p<0.001) and also GLP-1 gene expression (p<0.05). Conclusion: These findings demonstrate that administration of 7-Hydroxy-2-(4-hydroxy-3-methoxyphenyl)-chromen-4-one from Swietenia macrophylla King seeds, improved beta cell function through reducing oxidized LDL and increased GLP-1 expression.
  Prasetyastuti , Adhi Tanjung Laksono , Ricky Taufiqurrohman , Tantra Dewi Rahardyanti , Gandhi Anandika Febryanto , Yan Mahmud Fau , Sri Herwiyanti , Ngadikun and Sunarti
  Background and Objective: Carbon tetrachloride (CCl4), a carcinogenic compound commonly used in household cleaning agents and spot removers can cause free radical-mediated liver damage. Recent research shows polyphenols in pomegranate (Punica granatum L.), rich in exogenous antioxidants, may protect the liver from hepatotoxic damage. This study aimed to investigate the effect of pomegranate juice on CCl4-induced liver damage in a rat model. Materials and Methods: A total of 25 rats, aged 8 weeks and weighing an average of 200 g were used. They were divided into 5 groups as follows: (1) Normal, (2) Hepatotoxic rats and (3), Hepatotoxic rats with pomegranate juice 0.4 mL/200 g body weight (BW) (4) Hepatotoxic rats with pomegranate juice 0.8 mL/200 g BW and (5) Hepatotoxic rats with pomegranate juice 1.2 mL/200 g BW, respectively. Hepatotoxicity was done by intraperitoneal injection of 0.5 mL–1 CCl4 kg–1 BW for 5 days. Before and after 2 weeks administration of treatment, blood samples were collected for assessment of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (γ-GT) and malondialdehyde (MDA). At the end of the study, the animals were euthanized and the livers were used for histopathological assessment. Data were analyzed by paired t-test and one-way ANOVA followed by the Games-Howell test. Results: Supplementation with three different doses of pomegranate juices in CCl4-induced rats significantly reduced the serum levels of ALT, AST, ALP, γ-GT and MDA (p<0.05) and pomegranate juice as much as 0.8 and 1.2 mL/200 g BW reduced liver damage in CCl4-induced rats. Conclusion: According to findings of this study supplementation with pomegranate juice can improve liver damage induced by CCl4 in rats.
  Prasetyastuti , Mochammad Willy Pratama Anthony , Nuha Aulia Rahman , Ngadikun and Sunarti
  Diabetes mellitus is a metabolic disorder that is characterized by hyperglycemia and associated with increased oxidative stress. The polyphenol property in pomegranate juice was reported to have significant antioxidant effect. The present study aims to investigate the hypoglycemic and antioxidative effect of pomegranate juice in streptozotocin-induced diabetic rats. Twenty healthy male rattus norvegicus with age of eight weeks and weighing at average of 200 g were randomly assigned into four groups. Diabetic models were induced by using 60 mg/kg body weight (BW) streptozotocin (STZ) intraperitoneally. The rats were allocated into four groups, group treated with 0.1 mg/kg BW glibenclamide, groups supplemented with 1, 2 and 4 mL/200 g BW pomegranate juice, respectively. Evaluation of hypoglycemic and antioxidative effect were performed by measuring blood glucose level, lipid peroxidation, including malondialdehyde (MDA) and advanced glycation end-product (AGE) before and after treatment. Lipid profile was also measured before and after treatment. Treatment with glibenclamide and supplementation with three different doses of pomegranate juice in diabetic rats were significantly reduced blood glucose level. Supplementation with 2 mL/200 g BW pomegranate juice was significantly reduced MDA concentration, whereas the AGE concentration was not significantly decreased by pomegranate juice supplementation. Lipid profile related to total cholesterol and triglyceride levels were not significantly reduced. Furthermore, high density lipoprotein was not increased significantly after each treatment. Pomegranate juice has hypoglycemic and antioxidative effects in streptozotocin-induced diabetic rats.
  Prasetyastuti , Singgih Setiawan , Yuwinda Prima Ardelia , Tsurayya Fatma Hanifah , Yunardi Singgo and Sunarti
  Background: Retinol Binding Protein 4 (RBP4) is adipocytokine that it’s secretion and expression in adipose tissue is highly correlated with glucose uptake and insulin sensitivity. Increasing of RBP4 in type 2-diabetes mellitus correlate with insulin resistence. Swietenia macrophylla king contain alkaloid, saponin and flavonoid. Objective: The aim of this study is to investigate the effect of 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline alkaloid compound of Swietenia macrophylla king seed to lipid profile and liver tissue RBP4 expression in type 2-diabetes rats. Methodology: Twenty five adult rattus norvegicus were used, five in each group. Group I: Diabetic rat (untreated), group II: Diabetic rat receive glibenclamid, group III: Diabetic rat receive 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline dose 10 mg/200 g b.wt., day–1, group IV: Diabetic rat receive 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline dose 20 mg/200 g b.wt., day–1 and group V: Diabetic rat receive 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline dose 40 mg/200 g b.wt., day–1. Blood samples were analysed for blood glucose, cholesterol, low density lipoprotein, triglyceride and high density lipoprotein level before and after 4 weeks treatment. At the end of the experiment sample of liver tissue were removed for immunohistochemical analysis. Results: Blood glucose level decreased significantly (p<0.05) in group II, III, IV and V. Cholesterol level decreased non-significantly (p>0.05), Low Density Lipoprotein (LDL) decreased significantly (p<0.05) at dose 40 mg/200 g b.wt. High Density Lipoprotein (HDL) level increased significantly (p<0.05) at doses 10 and 20 mg/200 g b.wt. Conclusion: However, the expression of Retinol Binding Protein 4 (RBP4) decreased after treatment with 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline. These results demonstrated that 3,6,7-trimethoxy-4-methyl-1,2,3,4-tetrahydro-isoquinoline increased HDL and decreased LDL and RBP4 expression.
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