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Articles by Prapansak Chaveerach
Total Records ( 3 ) for Prapansak Chaveerach
  Preeyaporn Surachon , Peerapol Sukon , Prapansak Chaveerach , Panya Waewdee and Chaiyaporn Soikum
  Salmonellosis in humans caused by consumption of contaminated poultry products with Salmonella enteritica serovar enteritidis (S. enteritidis) is still a public health problem. Many efforts have been developed to eradicate or reduce Salmonella loads in poultry industry. Use of normal microbiota (e.g., Lactic Acid Bacteria (LAB)) against the pathogen is an alternative of antibiotics used and is under extensive investigations. Therefore, the objective of this study was to screen and identify the LAB strain showing the greatest growth inhibition against S. enteritidis. LAB were isolated from chicken ceca of five clinically healthy broilers (age, 42-50 days). The bacteria were grown in MRS broth and on the plate with selective media Rogosa agar. For screening of the inhibitory effects of the isolated LAB against S. enteritidis, we used disc diffusion and agar well diffusion methods. In this study, 56 isolates exhibited inhibitory effect against S. enteritidis but only thirteen isolates producing a clear zone as large as 19 mm or greater were selected for acid tolerance test. In this test, three isolates did survive at pH 2.5 for 18 h but only 1 isolate was subjected for evaluation by coculture with S. enteritidis and for 16S rDNA sequencing. This isolate was able to grow in the coculture medium and at the same time, inhibited the growth S. enteritidis. This isolate was identified as Lactobacillus salivarius TP4.2-2.
  Panya Waewdee , Peerapol Sukon , Prapansak Chaveerach , Preeyaporn Surachon and Chaiyaporn Soikum
  Lactobacillus salivarius strain LP 4.2-2 (L. salivarius LP 4.2-2) isolated from chicken cecum can strongly inhibit Salmonella enteritidis (S. enteritidis) in vitro but little is known about its effect on prevention of this pathogen in live chickens. Therefore, the main objective of this study was to determine whether a single dose of L. salivarius LP 4.2-2 given at low or high dose by oral or cloacal route would prevent S. enteritidis infection in young broilers in addition, effects of the experimental treatments on total bacterial count in cecal contents, body weight, organ weight and intestinal length in chicks were determined. In this study, 240 of 1 day old male broiler chicks were randomly assigned to 6 groups of 40 chicks each. Chicks in each group were housed separately in a cage (size 1.5x1.5 m2). At 1 day of age, each group received none, 104 or 1010 (cfu/chick) of L. salivarius LP 4.2-2 by either oral or cloacal route. At 2 days of age, all chicks except those in one group (a negative control) were challenged orally with 104 (cfu/chick) of S. enteritidis. At 3 days of age, a half number of chicks in each group (n = 20/group) were randomly selected for the detection of S. enteritidis infection in cecal tonsils. Other parameters such as total bacterial count, body weight and intestinal length were also measured. The remaining chicks were allowed to grow until 9 days of age and then the procedures for measuring each parameter were done the same as those described above. The results showed that at 3 days of age, rates of S. enteritidis infection were lower in all groups administered with L. salivarius LP 4.2-2 than in a positive control group (13/20 or 65%-17/20 or 85% versus 19/20 or 95%). However, at 9 days of age, rates of S. enteritidis infection were high in all groups (95-100%), except in a negative control (0%). No significances were seen in total bacterial counts and in body weights between groups either at 3 or 9 days of age. After adjusted for body weight, weights of most internal organs in all groups and total lengths of intestine in most groups did not differ significantly. In conclusion, a single dose of L. salivarius cannot prevent S. enteritidis infection in all chicks but it can reduce rate of the infection in 3 days old chicks. However, the preventive effect is diminished over time.
  Arunrat Chaveerach , Nattapong Srisamoot , Suporn Nuchadomrong , Nison Sattayasai , Prapansak Chaveerach , Alongkoad Tanomtong and Krit Pinthong
  The genetic relationship of 20 Carnivora species in Thailand was determined based on sequence analysis of the Internal Transcribed Spacer (ITS) region of ribosomal DNA. Aligned sequences of the complete ITS region obtained from the 20 taxa and two primate outgroups resulted in 890 characters with 710 variable sites. Genetic distances and a phylogenetic tree were constructed from comparisons of ITS sequences using the Neighborjoining method. The dendogram demonstrates that the 22 taxa can be clearly grouped in six clusters: Mustelidae, Ursidae, Canidae, Felidae, Viveridae and Hylobatidae. Of these clusters, the 20 Canivora species are clustered together and the superfamilies Caniformia and Feliformia can be separated apart, whereas the outgroup Hylobatidae is segregated from the canivora. In superfamilies Caniformia, the families Mustelidae, Ursidae and Canidae are clustered together. In superfamilies Feliformia, the family Felidae is clustered with Viverridae. The phylogenetic tree of Viverridae species does not completely match the classification based on morphological characters. The Paradoxurinae, Arctictis binturong was grouped into the Viverrinae while the other Paradoxurinae, Paradoxurus hermaphroditus, Paguma larvata and Arctogalidia trivirgata are clustered together.
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