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Articles by Pornthap Thanonkeo
Total Records ( 2 ) for Pornthap Thanonkeo
  Haruthairat Kitwetcharoen , Sudarat Thanonkeo , Preekamol Klanrit and Pornthap Thanonkeo
  Background and Objective: Bioactive compound production has been facing many challenges including a long cultivation time, the instability and the impurity of the products from natural plants. This study aimed to produce bioactive compounds from K. parviflora by using plant cell culture system and determine their antioxidant potential. Materials and Methods: Callus was induced from different organs of the plant including the shoots, rhizomes and roots using Murashige and Skoog (MS) medium supplemented with various Plant Growth Regulators (PGRs). Fast-growing friable calli from all explants were selected and used to establish cell suspension cultures. The biomass production, the accumulation of total phenolics, total flavonoids and the antioxidant activity of the cell culture extracts were determined and compared with those from intact rhizomes of K. parviflora. Results: All cell suspension cultures of K. parviflora exhibited high biomass yields. Furthermore, the productivities of the phenolics and flavonoids from K. parviflora cell cultures were also significantly higher than those from the intact rhizomes. The antioxidant capacity of the cell culture extracts was found to be comparable with that detected in the intact rhizomes of this plant. Conclusion: Based on the high biomass yield, high bioactive compounds productivity and antioxidant capacity, K. parviflora cell culture system displayed great potential for commercial-scale production. Further investigations on the multiple biological properties of the bioactive compounds produced by K. parviflora cell suspension cultures as well as the cytotoxicity test will be explored.
  Pornthap Thanonkeo , Kaewta Sootsuwan , Vichai Leelavacharamas and Mamoru Yamada
  Heat and ethanol had an affect not only on growth and cell viability of an obligatorily fermentative Gram-negative bacterium Zymomonas mobilis, but also on protein synthesis. Analysis by SDS-polyacrylamide gel electrophoresis revealed pronounced increasing of two dominant proteins designated as groES and groEL. Molecular cloning of the gene encoding groES and groEL was performed by PCR technique using specific primers synthesized based on the Z. mobilis groESL gene. Sequencing analysis of 2179 bp led to the detection of two open reading frames encoded for 95 and 549 amino acids, respectively. The deduced amino acid sequence of the Z. mobilis groES and groEL shows a high degree of identity with other. The strongly conserved carboxyl-terminus Gly-Gly-Met motif and two small segments, which appear more conserved between ethanol-producing organisms, were found, suggesting that their may be related to stability of protein under heat or ethanol stress. Induction of groES and groEL occurs in response to heat and ethanol, but not to salt stress.
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