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Articles by Pitipong Thobunluepop
Total Records ( 8 ) for Pitipong Thobunluepop
  Phyu Thaw Tun , Pitipong Thobunluepop , Ed Sarobol and Tanee Sreewongchai
  This study aimed to compare different cultivation techniques and plot levels on macronutrient utilization of lowland rice grown on acid sulfate soil for sustainable production. Cultivation technique (Modified Cultivation (MC) technique and Conventional Cultivation (CC) technique) and plot level (upper plot and lower plot) were experimental factors. Under MC technique, farmer applied 187.5 kg ha-1 of compound fertilizer (16-20-0) and 18.75 kg ha-1 of KCl (0-0-60) at planting, incorporated the previous rice stubble, transplanted rice seedlings and followed 2 weeks flooding and 1 week completely drainage system. Under CC technique, farmer applied 312.5 kg ha-1 of 16-20-0 compound fertilizer at planting, burned the previous rice stubble, broadcasted rice seeds and followed continuous flooding throughout the growing period. Upper plot was directly irrigated from drainage canal and lower plot was irrigated with the drained water from upper plot. Compared with CC technique, MC technique improved soil macronutrients content, especially N and P improving utilization of those nutrients by rice plant. Utilization of most macronutrients by rice was not statistically affected by plot level. It was suggested that farmers should follow MC technique to meet higher macronutrient utilization of lowland rice for sustainable production under acid sulfate soil.
  Natthida Trakunpaisan , Pitipong Thobunluepop , Sutkhet Nakasathien , Sukumarn Lertmongkol , Damrongvudhi Onwimol and Michael Bredemeier
  Background and Objective: Maize seed production systems are nowadays used to try to conduct early harvests and thereafter submitted for seed processing and marketing within a short period of time. Thus, the experiment was aimed to evaluate the effect of harvesting time and drying management on maize storability and seed vigor after early harvested. Materials and Methods: The experimental design was arranged in 2×4 factorial in Randomized Complete Block Design (RCBD) with four replications. The effect of two different harvesting times were H1: 100 Day after emergence (DAE, R5), H2: 110 DAE (R6) and four drying temperatures (T1: 30°C, T2: 35°C, T3: 40°C, T4: 45°C) were observed. Then, seed qualities and vigor were tested. Results: The H2 had the highest speed of germination. At 0 month, the highest germination percentage, speed of Germination, shoot length, Root Length (RL), Seedling Dry Weight (SDW) and seedling growth rate were obtained at 45°C. At 6th month of storage, the highest germination percentage, AA-test, RL and SDW were obtained at 35°C. Conclusion: The experiment could be concluded that seed drying at 45°C can be used for immediate use of seeds without storage. For storability, the anticipation of harvest and seeds drying at temperatures of 35°C result in maize seeds that have the ability to grow into normal seedlings in field conditions.
  Wilasinee Chitbanchong , Vicha Sardsud , Kanda Whangchai , Rumphan Koslanund and Pitipong Thobunluepop
  The effects of sulphur dioxide, in combination with, storage temperatures on postharvest decay, pericarp browning and physiological ultrastructure changed of the Longan fruit cv. daw were studied. The treatment of fresh the Longan fruit with SO2 fumigation combined with the suitable storage condition improved the overall the Longan fruit quality, especially on inner and outer peel tissue and aril color than no SO2 treatment, while no SO2 treatment showed the dark color of inner and outer peel of the Longan fruit was appeared, this was correlated with the increasing of polyphenol oxidase (PPO) activity. Moreover, the main factor affected Longan fruits quality was storage duration, the increasing of weight loss, pH value of both peel and aril, PPO activity, especially on the changing of dark-red color of peel was observed after long term of storage. However, the sulphite residues could detect immediately after SO2 treatment in all part of the Longan fruit, especially on peel tissue, but the residues was significantly decreased along the storage durations. On the other hand, Scanning Electron Microscope (SEM) evaluation found that the surface cracking was also impair the physiological function of the cuticle and increasing water permeability, which may cause water soaking at the inner side of the peel. The injured cell would accelerate the oxidation of phenolic substances and the oxidative products resulted in dark color of inner and outer peel. Therefore, the combination sulphur dioxide fumigation with controlling the optimum of storage temperature could control of postharvest decay and browning.
  Mgaya Athumani Maumba , Pitipong Thobunluepop , Tanee Sreewongchai , Ed Sarobol , Damrongvudhi Onwimol and Nantawan Sarobol
  Background and Objective: Rapid germination is very important for better plant growth and development under stress condition. The increase in soil and water temperature reduces seed germination ability, germination speed and hence resulting into poor seedling vigor and plat growth. This study was conducted to investigate to what extent the seed enhancement technique helped to improve the rice seed germination and seedling establishments on the effect of high soil temperature stress. Methodology: Four rice seeds varieties were primed with PEG 6000, hydropriming and non-primed seeds were used as a control. After priming, germination test under soil temperature stress of 36°C (room-control), 40, 43 and 46°C was performed in greenhouse condition to evaluate the stress effect on germination and seedling quality. Results: The results showed highly significant difference on maximum germination (max G, %), mean germination time (MGT%), time to attained 50% germination (T50%), germination energy (GE), shoot and root growth and development at different soil temperature stress between primed and non-primed seeds. Conclusion: Primed seed promote rapid germination as well as shoot and root growth under high soil temperature (40-43°C). However, growth and development were ceased at above 43°C. Furthermore, high soil temperature and priming techniques promoted root and shoot growth than to non-primed seeds.
  Paul Hategekimana , Pitipong Thobunluepop , Ed Saorobol and Nantawan Sarobol
  Background and Objective: Drought stress severely affects corn growth and development during its whole life cycle by altering cell division and leading to changes in plant morphology, reducing anthesis-silking interval and consequently results in poor yield. The aim of this research was to evaluate the adaptability of corn cultivars under various drought stress conditions at different growth stages. Materials and Methods: This research was conducted in green house condition during April to September 2017 at the Department of Agronomy, Faculty of Agriculture and Kasetsart University (KU). Four corn cultivars (SW4452, NS-3, NT6248 and NT7328) were tested under factorial in randomized complete block design (RCBD). There were three blocks where drought stress was executed in block wise. The block one executed at vegetative stage (30DAP), block two at flowering stage (60DAP) and block three at grain formation stage (76DAP).Drought stress adaptability of those cultivars at seedling stage was tested under growth chamber where germination data were collected on 21 days after germination. The physiological, morphological, roots properties and crop yield data were collected. Results: The result on maximum germination and T50 percentage showed highly statistical significant different at p<0.05 level for NT7328 and NT6248, respectively. The number of roots, root length, roots fresh and dry weight and root-shoots ratio was significantly different for Ns-3. Furthermore, the yield production (t h1) according to the period imposed to drought stress were 4.06, 3.69 and 2.85 t h1 for vegetative, flowering and grain formation stage. However high yield value within cultivars were 3.7, 3.4 and 2.6 and 4.2 t h1 for SW4452, NS-3, NT6248 and NT7328, respectively. Conclusion: Finally the result showed high drought adaptability to NT7328 and SW4452 while NT6248 cultivars was seems to be vulnerable to drought stress.
  Pitipong Thobunluepop
  Presently, chemical seed treatments are in discussion due to their directly or indirectly impacts on human health or other living organisms. They may also negatively affect the ecosystem and the food chain. In rice seeds, chemicals may cause phytotoxic effects including seed degradation. Eugenol is the main component of clove (Eugenia caryophillis) oil, which was proved to act simultaneously as bactericide, virocide and especially fungicide. The in vitro study was aimed to compare the inhibitory effect of the following seed treatment substances against seed borne fungi and their shelf-life during 12 months of storage; conventional captan (CA), chitosan-lignosulphonate polymer (CL), eugenol incorporated into chitosan-lignosulphonate polymer (E+CL) and control (CO). The obtained results of fungi inhibition were classified in three groups, which showed at first that CA treatment led to a better, i.e., longer, inhibitory effect on Alternaria padwickii, Rhizoctonia solani, Curvularia sp., Aspergillus flavus and Aspergillus niger than E+CL. Secondly, E+CL coating polymer showed the longest inhibitory effect against Bipolaris oryzae and Nigrospora oryzae compared to CA and CL coating polymer. Finally, both CA and E+CL coating polymer had non-significant difference inhibitory effect on Fusarium moniliforme. The variant of CL coating polymer for seed coating was only during the first 6 months of storage able to inhibit all species of the observed seed borne fungi, whereas CA and E+CL coating polymer were capable to inhibit most of the fungi until 9 months of storage.
  Pitipong Thobunluepop
  This study was aimed to evaluate the several chemical compounds of relatively composite structure with antifungal activity from Thai local medical plants. The antifungal activity of Stemona curtisii HK. f., Stemona tuberose L., Acorus calamus L., Eugenia caryophyllus, Memmea siamensis Kost. and an eugenol active compound were studied in vitro. Four pathogenic seed borne fungi, Alternaria solani, Colletotrichum sp., Fusarium moniliforme and Rhizoctonia solani were used as target organisms. The agar overlay technique and spore inhibition techniques were applied for the determination of their essential oil and active compound antifungal activity at various concentration; 0.10, 0.25, 0.50 and 1.00% (v/v) and untreated as control (0% v/v). Eugenol active compound showed the strongest antifungal activity on all species of tested fungal species. On the other hand, the antifungal activity of those bio-fungicides was lined up into a series from strong to low, as follows: Eugenia caryophyllus>Acorus calamus Linn.>Stemona tuberosa L.>Stemona curtisii Hk.f, while Mammea siamensis Kost. could not control any fungal species. Moreover, after eugenol application, lysis of spore and inhibition of mycelium growth were detected. Microscopic analysis exhibited complete lysis of spores after 24 h at a concentration of 1.00% v/v. Moreover, at the same concentration and 96 h incubation the mycelia growth was completely inhibited.
  Ranu Tumpaung , Pitipong Thobunluepop , Pasajee Kongsil , Damrongvudhi Onwimol , Sukumarn Lertmongkol , Ed Sarobol , Wilasinee Chitbanchong and Elke Pawelzik
  Background and Objective: Quinoa grain contained saponin in pericarp, which causes bitter flavor. After harvesting, quinoa grain is required to remove saponin before being consumed. Thus, this study aimed to study post harvest management of grain processing on the saponin and nutrition value of quinoa grain. Materials and Methods: The experiment was arranged in a Completely Randomized Design (CRD) with three replications and saponin removal technique with milling process (T1-T2) and reagent washing (T3-T8) were used as experimental treatments comparing with non-process grains as a control (T9). Nutrition analysis was an indication of quality in post-process quinoa grain. Results: The experiment found that T1, T3, T4, T5, T6 and T7 could reduce saponin content significantly different from T9, while T2 and T8 still show high saponin content when compared with T9. T2 and T4 techniques could maintain most of the nutritional value of quinoa grain when compared with control (T9). Conclusion: Finally, this experiment could be concluded that quinoa was washed by alkaline solution (pH 8) for 8 min by three times (T4) could be an optimum of saponin removal technique. This technique not only removed saponin but could also maintain quinoa grain qualities. Meanwhile, it potentially reduced for 66.03 percent of saponins content when compared with control treatment, which did not change in protein content, flavonoid content, moisture content, starch content, phenolic content and color (L*) of quinoa grain.
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