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Articles by Pingkan Aditiawati
Total Records ( 7 ) for Pingkan Aditiawati
  Noor Rahmawati , Ariesta Rakhmat Isfandito , Dea Indriani Astuti and Pingkan Aditiawati
  This study aims to isolate and identify endophytic fungi from tissues of stem wood at different age of Toona sinensis and test the antioxidant activity produced from the culture of these fungi. Twenty five isolates were obtained and identified into 7 types of fungi classes; compromising of Trichoderma sp., Aspergillus sp., Rhizopus sp., Cladosporium sp., Penicillium sp., Alternata sp. and Chepalosporium sp. The fungus were then cultured into 100 mL of PDB medium, agitated at 150 rpm, at 28°C for 1 week. Cultures containing antioxidant compound were extracted using n-hexane and ethyl acetate and the antioxidant activity was determined by DPPH method. The ethyl acetate extracts of culture filtrate has higher antioxidant activity comparing than n hexane extracts. Among the 25 isolates, the antioxidant activity of n-hexane extract of culture Penicillium sp., number 1, isolated from 5 years surian branch had the greatest DPPH radical scavenger of 27.79% and the lowest one was 22.29% obtained from Cladosporium sp. and Trichoderma sp., number 8. Whereas antioxidants compound extracted by ethyl acetate of Rhizopus sp., number 2, from 5 months stem shoot had the greatest DPPH radical scavenger of 81.01% and the lowest one is from isolate Aspergillus sp., number 1 from white branch shoot of 5 months which had an activity of 68.72%. This result indicated that surian have many kind of endophytic fungus and some of are potential for producing antioxidant compound.
  Aminudin Sulaeman , Rudi Dungani , Nuruddin Nurudin , Sri Hartati , Tati Karliati , Pingkan Aditiawati , Anne Hadiyane , Yoyo Suhaya and Sulistyono
  The properties of bamboo are unique compared to solid wood and other ligno- and non-lignocellulosic materials especially for manufacturing, designing and construction usages. Recent technological advancements for bamboo processing has proven the positive advantages of bamboo for various interior and exterior applications including furniture, bio-composites, packaging, transport, building and so on. The variability in size, length and diameter of bamboo, its growth and production is a big challenge for their applications as bio-based material along with durability. Since bamboo has a low durability, protection against biotic and abiotic degradation. Since it has vital importance for its longer service life. Preservation and modification treatment of bamboo are therefore regarded as a necessity. Those preservation and modification techniques would ensure the quality and durability of bamboo resulting sustainability and advanced engineering utilization of bamboo. This article reviews the preservation and modification techniques of bamboo which are crucial for advanced products manufacturing and utilization. The article also summarizes the importance of preservation and modification process, its principles and the challenges in quality and durability enhancement of bamboo products. At the end of the article, applications specially the modern one has also been discussed along with its further advancement.
  Pingkan Aditiawati , Akhmaloka , Dea Indriani Astuti , Sugilubin and Megga Ratnasari Pikoli
  Coal as fuel should be necessarily pre-treated by desulfurization in order to prevent excessive emissions of sulfur dioxide, a precursor of acid rain. Organic sulfur in coal can be eliminated by microbial action through the technology known as biodesulfurization. Source of microorganisms in the present study was coal mine soil in which microorganisms have been adapted to use the sulfur in coal. Coal mine in South Sumatra was choosen as source of microorganisms in this study, because it is an area in Indonesia with the largest of subbituminous coal reserves. The microorganisms were activated as mixed culture by culturing the soil sample in mineral salt medium containing subbituminous coal as the sole sulfur. Desulfurization activities were examined by using three variations of the initial coal concentration, i.e., 10, 15 and 20% weight per volume. Growth and activity of the mixed culture on the subbituminous coal were monitored by measuring of medium pH, cell concentration, sulfate and organic sulfur concentration. The result showed that desulfurization activity of the mixed culture on 15% of coal was able to reduce sulfur up to 82.36%. Isolation and identification of the mixed culture based on genotypic and phenotypic characterizations revealed that members of the mixed culture were identified as genera of Enterobacter, Lelcersia and Bacillus. Observation on growth curves showing that the culturable isolates grew in at least three overlapping stages when using coal as sulfur source suggested that the members of the consortium worked alternately on coal as substrate.
  Keukeu K. Rosada , Nur F. Afianti , Dea Indriani Astuti , Gede Suantika and Pingkan Aditiawati
  Bacterial communities in planktonic cells and biofilm at Saguling Hydro Power were investigated using Denaturing Gradient Gel Electrophoresis (DGGE) technique. Physicochemical characteristic of aqueous medium indicated that the water source was classified as moderately polluted (eutrophic). The study inferred that isolates in biofilm mostly derived from the planktonic cells and the Mann-Withney U tests showed that there was no significantly difference between the bacterial communities of planktonic cells and biofilm. The 16S rRNA sequences revealed that bacteria recovered from the planktonic cells were affiliated with Betaproteobacteria and Bacteroidetes phyla. Meanwhile, the sequences bacteria revealed from biofilm were closed to Betaproteobacteria, Alphaproteobacteria and Gammaproteobacteria groups. Overall, the majority of microbes identified from the two samples were belong to Betaproteobacteria group.
  Keukeu K. Rosada , Navisan Najia , Rahma Widya Ningrum , Dea Indriani Astuti , Gede Suantika and Pingkan Aditiawati
  Background and Objective: Biofilm plays an important role in causing microbial corrosion. One of prerequisites for microbes as energy source to grow and to form biofilm is carbon source. The purposes of this study were measuring the ability of biofilm community sampled from Saguling hydro power in utilizing carbon sources and assessing culturable heterotrophic of bacterial community from those biofilm. Materials and Methods: Biolog EcoPlateTM and culture-dependent approach were used to assess biofilm community. Results: Heterotrophic bacteria in biofilm have the ability to use 30 different carbon sources consistently. The source of carbon used by this community at the highest rate are N-acetyl-D-glucosamine from carbohydrates group, 2-hydroxybenzoic acid from carboxylic and kenotic acids, glycogen from polymers group and L-serine from the amino acid group at the rate of 0.16, 0.05, 0.14 and 0.09 absorbance U h–1 respectively. Whereas the carbon sources with high consumption are as follow: Pyruvic acid methyl ester, β-methyl-D-glucoside, D-mannitol and N-acetyl-D-glucosamine from carbohydrates group; D-galacturonic acid, 2-hydroxybenzoic acid, D-glucosaminic acid and D-malice acid from the group of carboxylic and kenotic acids; tween 40 and 80 as polymers; L-argentine, L-asparagines, L-serine, L-heroine and glycol-L-glutei from amino acids group. Furthermore, culturable bacterial community of those biofilm were dominated by Gram-negative bacteria, consisted of five common/heterotrophic bacteria, two manganese bacteria, two nitrifying bacteria, three iron bacteria and three sulfate reducing bacteria. Conclusion: Bacterial community of biofilm from Saguling hydro power which were dominated by Gram-negative bacteria have the ability to use various carbon sources and degrade glycogen.
  Gede Suantika , Alissa Diany Putri , Yovita Astuti Djohan , Fahma Fiqhiyyah Nur Azizah , Dea Indriani Astuti and Pingkan Aditiawati
  Background and Objective: The impact of environmental factors on the B vitamins (B1, B2, B6 and B12) content of marine diatom Skeletonema costatum has not yet been evaluated so far. Here, we aim to optimize the B vitamins production (vitamin B1, B2, B6 and B12) through culture of S. costatum on different salinity levels as well as light intensity exposures. Materials and Methods: The marine diatom Skeletonema costatum was cultured in different salinity levels (24, 29 and 34 g L–1) to optimize B vitamins production, prior to exposure to different light intensity levels (20, 34 and 47 μmol m–2 sec–1) for 3 days in f/2 medium. Results: Twenty four grams per liter salinity exposure produced the highest vitamin B1, B6 and B12 content of 90.08±2.6, 410.03±12.97 and 61.22±27.67 μg g–1, respectively. When cultivated either below or above light intensity of 34 μmol m–2 sec–1, vitamin B1, B6 and B12 content decreased. The highest total B vitamins obtained at 34 μmol m–2 sec–1 was 563.46 mg L–1 (vitamin B1: 90.08±1.48 μg g–1, vitamin B2: 2.87±0.79 μg g–1, vitamin B6: 410.03±12.97 μg g–1 and vitamin B12: 61.22±27.67 μg g–1). Conclusion: Optimum growth, biomass and total B vitamins produced was achieved by culturing at salinity of 24 g L–1 and light intensity 34 μmol m–2 sec–1. So far, optimum growth, biomass and total B vitamins produced was achieved by culturing at salinity of 24 g L–1 and light intensity 34 μmol m–2 sec–1. Note that when aiming at high vitamin productivities, it is better to culture S. costatum in a two step process: A nutrient sufficient phase in optimum environmental growth conditions to produce enough cells (e.g., salinity, illumination, pH, temperature, supply of CO2 and nutrients etc.), followed by suitable stress to stimulate B vitamins synthesis in a controlled manner.
  Gede Suantika , Magdalena Lenny Situmorang , Pingkan Aditiawati , Abdul Khakim , Shrikumar Suryanarayan , Sri Sailaja Nori , Sawan Kumar and Ferisca Putri
  Background and Objective: Several seaweeds have been reported to contain different bioactive compounds with antimicrobial activity, providing protection against certain infectious diseases in aquaculture production. This study aimed to explore the potential of red seaweed Kappaphycus alvarezii product as an alternative for anti-infective strategy and enhancement of salinity stress tolerance in shrimp (Litopenaeus vannamei) hatchery culture. Materials and Methods: Shrimp post-larvae were fed with Artemia nauplii, either enriched or not enriched with seaweed paste then challenged with Vibrio at day 5 and 8. Comparison of shrimp growth and survival between treatments following salinity stress test and Vibrio challenge were done using one-way analysis of variance analysis. Results: Shrimp fed with seaweed-enriched Artemia resulted in higher survival after Vibrio challenge (90.2±7.0%) compared to shrimp fed with non-enriched Artemia (77.7±3.1%). Shrimp fed with non-enriched Artemia resulted in lower growth after Vibrio challenge (9.65±0.20% b.wt., day–1) compared to the non-challenged group (10.34±0.25% b.wt., day–1). In contrast, there was no difference in the growth of shrimp fed with seaweed-enriched Artemia with or without Vibrio challenge (10.51±0.19 or 10.80±0.28% b.wt., day–1, respectively). The shrimp fed with seaweed-enriched Artemia also obtained a higher survival following salinity stress test (94±2%) compared to shrimp fed with non-enriched Artemia (79±4%). Conclusion: Overall results suggested that red seaweed K. alvarezii by-product enrichment on live feed Artemia for shrimp post-larvae during the hatchery phase can provide protection against V. harveyi infection, improve the growth of shrimp when exposed to pathogenic V. harveyi and also allow higher salinity stress tolerance. Further evaluation on the effect of seaweed by-products dietary supplementation in the nursery and grow-out phases are undoubtedly required, to accurately evaluate the potential of seaweed by-product application as a growth and disease resistance promoting agent in those later phases as well.
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