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Articles by Ping Yang
Total Records ( 2 ) for Ping Yang
  Chiaki Endo , Zhifu Sun , Julian R. Molina , John R. Attewell and Ping Yang
  To compare gene expression levels of lung cancer by real time quantitative reverse transcription polymerase chain reaction (qRT-PCR), choosing appropriate control genes for normalization of RNA input is important. We examined whether frequently used control genes are really appropriate. Eighteen lung cancer tissue samples were used in this study. Gene expression levels were measured by qRT-PCR and compared to the data generated by a DNA microarray experiment. The expression levels of four housekeeping genes that are most frequently used as control genes for qRT-PCR published reports in lung cancer were examined. Four test genes (TP53, laminin γ2, TGFα and cathepsin L2) were also examined. These genes had significantly higher expression in the high aggressive disease group. When taken as single genes, the expression levels of these so called control genes (glyceraldehydes-3phosphate dehydrogenase, TATA box binding protein, β actin and β2 microgloblin) were not consistent among the tested samples. However, the geometric mean of the threshold cycle of the TATA box binding protein and β2 microglobulin showed less variation than that of the single control gene. These results indicate that normalization by multiple control genes is more appropriate for comparison of gene expression levels of lung cancer when using qRT-PCR analysis.
  Ping Yang , Ruofu Yang , Feng Shen , Xinyang Li and Wenhan Jiang
  To control the phase noise of two ytterbium fiber amplifiers, a coherent combination system based on an active segmented mirror (ASM) has been established in our laboratory. The ASM is controlled by a feed back control loop on the basis of a Peak Rate (PR) algorithm which is realized on a DSP+FPGA hardware control board. Experimental results indicate that when the control loop is off, the far-field interference pattern is blurred and dynamic, while when it is on, the far-field beams interference pattern achieves clear and stable. At two different output powers, the contrasts of the interference stripes are improved from 7% to 19% and 8% to 28% respectively.
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