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Articles by Ping Liu
Total Records ( 9 ) for Ping Liu
  Ping Liu , Xiao Lu Hou , Pei Nie , Yin Hua Han , Yun Fei Huang , Zhen Xing Zoun , Hui Fang Deng , Peng Song , Ming Li and Bao Xiang He
  Determination of Alanine aminotransferase (ALT) activity plays an important role in the diagnosis of liver function injury. Cows manifest hypernomic fat mobilization in early lactation which causes liver function injury and lead to variation of ALT activity. The study aim was to establish an advanced diagnostic method for determining the activity of ALT and analyze their dynamics characteristics and correlation in milk and blood plasma collected from postpartum dairy cows in early lactation. In this study, 35 healthy Holstein cows were involved. About 187 blood plasma and milk samples were collected from cows 4-10 weeks post-parturition. Samples were collected once weeks in the morning before the cows were feed. In the collected samples, the concentration of ALT was detected by spectrophotometer techniques. The results showed that: ALT activity was significantly lower in milk than that in blood plasma (p<0.05); ALT activity in both blood plasma and milk significantly increased and reached the peak at the same time at week 8 postpartum. According to the results of the study, there was significant positive correlation (r = 0.84, p<0.001) in the concentration of ALT between blood plasma and milk. Therefore, the outcome results suggest that ALT activity determination in milk can replace that in blood plasma in case of detection in the earlier lactation of cows days postpartum. This method may contribute to an extensive survey and monitoring systems.
  Kenneth H. Fife , Tze-Chiang Meng , Daron G. Ferris and Ping Liu
  Resiquimod, a Toll-like receptor 7/8 agonist developed as a topical treatment to decrease recurrences of anogenital herpes, induces proinflammatory cytokines that may delay lesion healing. Adults with frequently recurring anogenital herpes were randomized within 24 h of onset of a recurrence to vehicle or resiquimod 0.01% gel two times per week for 3 weeks. Subjects underwent daily lesion assessments and sampling for herpes simplex virus DNA PCR for 21 days or until investigator-determined healing of lesion(s). Eighty-two subjects with a mean age of 39 ± 10.5 years and a median of seven recurrences per year were enrolled in the study. The qualifying recurrence was positive by PCR for herpes simplex virus in 68% of subjects. No difference was observed between the vehicle (39 subjects) and resiquimod (43 subjects) groups with respect to time to healing (median of 7.0 days versus median of 6.5 days, respectively; Cox proportional hazard model ratio of 1.229; 95% confidence interval, 0.778 to 1.942; P = 0.376). The distributions of maximum severity scores for any investigator-assessed local skin signs and for subject-assessed local symptoms were similar between treatment groups (P = 0.807 and P = 0.103, respectively). For subjects with at least one positive PCR result, no difference was observed for time to cessation of viral shedding (median of 7 days versus median of 5 days for vehicle and resiquimod groups, respectively; Cox proportional hazard model ratio of 1.471; 95% confidence interval, 0.786 to 2.754; P = 0.227). Application of resiquimod 0.01% two times per week for 3 weeks did not delay the healing of genital herpes lesions or reduce acute viral shedding.
  Taorong Qiu , Shanshan Zhang , Hou Zhou , Xiaoming Bai and Ping Liu
  Aiming at the lack of highresolution and short-term (5, 5km and next 3 h) thunderstorm forecast model, the three thunderstorm forecast models based on Rough Sets (RS), Support Vector Machine (SVM) and RS-SVM are presented in this study through analyzing Rough set, Support machine vector and the characteristic of lightning forecasting. With the real data set, the models are tested and the experimental results are analyzed, which show that the RS-SVM based model is effective and moreover, acquires a higher forecast precision.
  Ding Zhou , Yi Zhang , Daxi Xue and Ping Liu
  Background and Aim: Cataracts have the highest incidence among eye diseases, particularly blindness. Quercetin as a major flavonoid in the diet, can prevent cataracts. This study was aimed at investigating the molecular mechanism of the protective effects of quercetin in selenium-induced cataracts in Wistar rat. Materials and Methods: Ten-day old Wistar rats were randomly divided into four groups, all treated by intraperitoneal injection with 20 μM kg–1 sodium selenite to induce cataracts. The groups were a model group with no further treatment (A), a heated bath group (B), an Alanine-Glutamine treatment group (1.125 g kg–1; group, C) and a quercetin treatment group (400 mg kg–1, i.p., D). Expression level of HSP70 was determined by quantitative real-time PCR and immunohistochemistry. 8-Hydroxy-2-deoxyguanosine (8-OHdG), superoxide dismutase (SOD) and catalase (CAT) were assessed by ELISA assay. Results: The selenium-induced cataract model in rats was established successfully. The expression level of HSP70 in the groups B and C was significantly higher than that in group A. In contrast, the expression level of HSP70 in group D was significantly lower than that in group A (p<0.05). 8-OHdG levels in lens epithelial cells of group D were significantly lower than those in the cells of group A (p<0.05). CAT and SOD activities were enhanced to a much greater extent in group D than in group A (p<0.05). Conclusion: Quercetin has a protective effect on selenium-induced cataracts and probably modulates the expression of HSP70 to maintain homeostasis of oxidative stress.
  Nianli Zou , Fangfang Zhao , Ping Liu , Sanjie Cao , Xintian Wen and Yong Huang
  The serotypes and genotypes of Infectious Bronchitis Virus (IBV) was mainly determined by the Spike (S)1 glycoprotein. A prevalent IBV strain ZY3 was isolated and the highly antigenic region of its S1 gene was amplified and expressed in Escherichia coli using the pET-32a (+) vector. The fusion protein, which was expressed at a high level was similar antigenically to the native S1 protein as determined by Western blot assay using rabbits polyclonal antibodies against IBV ZY3 strain. The fusion protein was also purified. This research lays the foundation for using this recombinant protein for development of indirect Enzyme-Linked Immunosorbent Assay (ELISA) for serum antibody detection or for production of monoclonal antibodies against prevalent IBV.
  Ping Liu , BaoXiang He , XianLing Yang , XiaoLu Hou , HaiYang Zhao , YinHua Han , Pei Nie , HuiFang Deng and Long Cheng
  Depressed appetite and reduced Dry Matter Intake (DMI) and feeding energy-dense diets for a long time around parturition of cows may lead to excessive lipid mobilization which causes the liver damage. This study was meant to determine the effects of postpartun enzymes metabolic status in Holstein cows. In this study, blood samples were during the whole experimental period, obtained from the jugular venepuncture from each animal on 1 week prepartum (week 1), days delivery (week 0) and 1st 9 weeks postpartum (week 1-9). They were analyzed for examining Aspartate aminotransferase (AST), Alanine Aminotransferase (ALT), Gamma-Glutamyltransferase (GGT), Alkaline Phosphatase (ALP) activity. The resultes showed a higher activity of AST which was determined in the 1-3 weeks than other’s. ALT activity indicated a statistically significant increase from the 5-7 weeks of lactation and activity in the 7th week postpartum periods significally reached to the peak. GGT activity in the antepartum 1 week until delivery day was significally lower in comparison with the first to reach the 9th weeks postpartum. ALP activity in the delivery day and 6-8 weeks significant increased in process. Therefore, the AST, ALT, GGT and ALP of enzyme activity which could be used significantly change in the blood plasma of Holstein.
  Ping Liu , Kui Li , Roberto P. Garofalo and Allan R. Brasier
  Respiratory syncytial virus (RSV) is a primary cause of severe lower respiratory tract infection in children worldwide. RSV infects airway epithelial cells, where it activates inflammatory genes via the NF-κB pathway. NF-κB is controlled by two pathways, a canonical pathway that releases sequestered RelA complexes from the IκBα inhibitor, and a second, the noncanonical pathway, that releases RelB from the 100-kDa NF-κB2 complex. Recently we found that the retinoic acid-inducible gene I (RIG-I) is a major intracellular RSV sensor upstream of the canonical pathway. In this study, we surprisingly found that RIG-I silencing also inhibited p100 processing to 52-kDa NF-κB2 ("p52"), suggesting that RIG-I was functionally upstream of the noncanonical regulatory kinase complex composed of NIK·IKKα subunits. Co-immunoprecipitation experiments not only demonstrated that NIK associated with RIG-I and its downstream adaptor, mitochondrial antiviral signaling (MAVS), but also showed the association between IKKα and MAVS. To further understand the role of the NIK·IKKα pathway, we compared RSV-induced NF-κB activation using wild type, Ikkγ-/-, Nik-/-, and Ikkα-/--deficient MEF cells. Interestingly, we found that in canonical pathway-defective Ikkγ-/- cells, RSV induced RelA by liberation from p100 complexes. RSV was still able to activate IP10, Rantes, and Groβ gene expression in Ikkγ-/- cells, and this induction was inhibited by small interfering RNA-mediated RelA knockdown but not RelB silencing. These data suggest that part of the RelA activation in response to RSV infection was induced by a "cross-talk" pathway involving the noncanonical NIK·IKKα complex downstream of RIG-I·MAVS. This pathway may be a potential target for RSV treatment.
  Guo-Dong Li , Xi Zhang , Rong Li , Yue-Dan Wang , Yan-Li Wang , Ke-Jun Han , Xiao-Ping Qian , Cheng-Gang Yang , Ping Liu , Qun Wei , Wei-Feng Chen , Jun Zhang and Yu Zhang
  CHP2 (calcineurin B homologous protein 2) was initially identified as a tumor-associated antigen highly expressed in hepatocellular carcinoma. Its biological function remains largely unknown except for a potential role in transmembrane Na+/H+ exchange. In the present study, we observed that ectopic expression of CHP2 promoted the proliferation of HEK293 cells, whereas knockdown of endogenous CHP2 expression in HepG2 inhibited cell proliferation. When inoculated into nude mice, CHP2 transfected HEK293 cells displayed markedly increased oncogenic potential. In analysis of the underlying molecular mechanisms, we found that like calcineurin B, CHP2 was able to bind to and stimulate the phosphatase activity of calcineurin A. In accord with this, CHP2-transfected cells showed increased nuclear presence of NFATc3 (nuclear factor of activated T cells) and enhanced NFAT activity. Finally, both accelerated cell proliferation and NFAT activation following CHP2 transfection could be suppressed by the calcineurin inhibitor cyclosporine A, suggesting an intrinsic connection between these events. Taken together, our results highlighted a potential role of CHP2 in tumorigenesis and revealed a novel function of CHP2 as an activator of the calcineurin/NFAT signaling pathway.
  Bo Zhao , Changxin Chen , Boluo Yadian , Ping Liu , Zijiong Li , Dong Xu and Yafei Zhang
  Ultrasonic nanowelding technique was used to improve the field emission properties of carbon nanotube (CNT) cathodes. Two kinds of welding heads were used and the effects of the head on the emission properties were studied. The results show that cathodes welded by Al2O3 flat head demonstrate excellent field emission properties with high emission current density and good current stability. The improved field emission performance is attributed to the reliable and low resistance contact between CNTs and metal substrates. Cathodes welded by steel matrix head show a lower turn-on electric field due to the protruding CNTs at the edge of the welded pits.
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