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Articles by Phalisteen Sultan
Total Records ( 5 ) for Phalisteen Sultan
  Phalisteen Sultan , A.S. Shawl , P.W. Ramteke , Arif Jan , Nahida Chisti , Neelofar Jabeen and S. Shabir
  A protocol has been developed for in vitro shoot proliferation from callus cultures of Podophyllum hexandrum Royle. Callus initiation occurs from root segments of established in vitro grown seedlings on Gamborg`s B5 media (half strength) supplemented with 2,4-D (0.5-1.5 mg L-1) and BA (0.2-1.0 mg L-1). The rhizome of this plant contains several important lignans and most important being podophyllotoxin, main precursor for anticancer drugs teniposide and ertoposide. In addition Podophyllum hexandrum has been reported to have radioprotection properties. Ruthless collection has led to the disappearance of this important medicinal plant from many areas of Himalayas. Thus there is need for immediate conservation of this important medicinal plant through tissue culture means. In the present study shoot proliferation occurs from callus cultures cultured on basal MS medium fortified with BA and IAA either alone or in combination (0.5-5.0 mg L-1) each. Regenerated shoots were rooted with high efficiency on MS medium fortified with activated charcoal (0.5-1.0%) and NAA (0.5-2.0 mg L-1). The rooted plantlets were transferred to green house in jiffy pots containing sand, soil and vermiculite in 1:1:1 ratio.
  Neelofar Jabeen , A.S. Shawl , G.H. Dar , Arif Jan and Phalisteen Sultan
  A protocol has been developed for in vitro shoot proliferation from callus cultures of Aconitum heterophyllum Wall. Callus initiation occurs from nodal segments on MS media fortified with NAA (0.5 mg L-1) and BAP (0.25 mg L-1). Callus was transferred on Msmediasupplemented with BAP (0.25 mg L-1) for shoot proliferation. The best response for shoot proliferation was obtained on MS media+ NAA (0.25 mg L-1) + BAP (0.5 mg L-1). The well-developed micro shoots were transferred to root induction media containing MS basalmedia + IAA (1.0 mg L-1). The rooted plantlets were finally transferred to green house for hardening and field transfer.
  Nahida Chishti , A.S. Shawl , Z.A. Kaloo , M.A. Bhat and Phalisteen Sultan
  Nodal explants of Mentha arvensis L. from naturally grown plants were cultured on MS basal medium fortified with various combinations of auxins and cytokinins. The best response for multiple shoot regeneration (4±1.58) and callogenesis was observed in MS+4.4 μM BAP. Whereas, explants cultured on MS+8.9 μM BAP+4.4 μM IAA exhibited the best direct multiple shoot regeneration (14±2.74) and rhizogenesis (1±0.70). The well developed micro shoots were successfully acclimated.
  Nahida Chishti , Z.A. Kaloo , A.S. Shawl and Phalisteen Sultan
  An efficient regeneration protocol has been developed for rapid and mass scale clonal propagation of Lavendula officinalis from shoot tip explants. For the induction of multiple shoot formation, the explants were cultured on Murashige and Skoog`s (MS) basal medium supplemented with different concentrations of cytokinins viz., BAP (0.5-2.0 mg L-1), Kn (0.5-2.0 mg L-1) and on different cytokinin plus auxin combinations viz., BAP (1.0-2.0 mg L-1+IAA (0.5-1.0 mg L-1) and Kn (1.0-2.0 mg L-1) + IAA(0.5-1.0 mg L-1). These shoot tips produced multiple shoots, with the best result in BAP (2.0mg L-1) whereby (45±6.40) shoots were produced/explant in 80% cultures in 4 weeks time. The regenerated shoots were subcultured on the best induction medium (BAP 2.0 mg L-1) after every 4 weeks so as to increase their number. These shoots rooted best in half strength MS basal medium fortified with IBA (1.0mg L-1) with 7-18 roots per shoot(Avg. 16±6.89) roots in 80% cultures in 4 weeks time. Rooted plantlets were shifted to the plastic pots containing mixture of sand:clay:vermiculite (1:1:1) and kept for two weeks under controlled conditions of temperature (25±2°C) and relative humidity 60%. Afterwards these plantlets were hardened in green house environment (Vista Biocell Limited ) for one month and finally shifted to field conditions were 70% plants survived.
  M.I. Shah , Phalisteen Sultan , A. Nasier , P. Williams , Arif Jan , M. Sajad , Suriya Rehman and A.S. Shawl
  The present study was conducted to see the effect of some fungicides viz., Carbendazim, Mancozeb, Sulphur and conjoint Carbendazim and Mancozeb against Fusarium oxysporum in the division of Microbiology and Mycology of this laboratory. Poisoned food technique was adopted for carrying out this experiment for measuring radial growth of the test fungus in cm. All the treatments significantly reduced the growth of the F. oxysporum udum as compared to control but it was observed that the growth of the fungus were significantly less in 10,000 ppm concentration as compared to 10, 100 and 1000 ppm concentration of fungicides. On comparative analysis of different fungicides tested Mancozeb showed maximum inhibition of F. oxysporum, udum as compared to other fungicides.
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