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Articles by Pengfei Qi
Total Records ( 2 ) for Pengfei Qi
  Shuai Su , Ning Cui , Yixin Wang , Aijun Sun , Pengfei Qi , Zimeng Chen , Peng Zhao and Zhizhong Cui
  Marek’s Disease Virus (MDV) Chinese strain GX0101 was the first reported recombinant MDV field strain with one Reticuloendotheliosis Virus (REV) Long Terminal Repeat (LTR) insert. REV LTR in the GX0101 genome increased the potential for horizontal transmission. REV LTR fragment has the biggest impact on SORF2 gene. Researchers detected the influence of SORF2 gene deletion on its horizontal transmission ability by real time PCR. The result shows that GX0101ΔSorf2 possesses similar replication ability with bac-GX0101 no mater on the level of lymphocyte or feather follicle. Although, researchers can detect MDV positive in feather follicles of sentinel in contact chickens at age 10 in both groups respectively, the number of SPF chickens infected with bac-GX0101 by contact is higher than the number of GX0101ΔSorf2 infection group. Therefore, SORF2 gene has certain effect on the horizontal transmission ability of the virus.
  Qing Chen , Pengfei Qi , Renlin Xu , James T. Tambong , Zeinab R. Djama and Wei Li
  Pseudomonads are of significant environmental importance as the members including plant and animal/human pathogens, xenobiotic degraders, plant growth promoters and biocontrol agents. Three typing methods were evaluated, utilising 143 strains of fluorescent pseudomonads isolated from maize-oat-alfalfa-alfalfa rotation plots in Canada, to assess their usefulness as tools to study the microbial community diversity. The methods used were repetitive element sequence-based PCR (rep-PCR) utilising BOX and Enterobacterial Repetitive Intergenic Consensus (ERIC) primers, another new PCR method using an engineered polymerase and 10-nucleotide “miniprimers” expands the scope of detectable sequences beyond those detected by standard methods using longer primers and Taq polymerase. Cluster analysis of the results clearly demonstrated the groups can be divided into two big clusters in line with the morphological variation. Rep-PCR proved to be highly discriminatory, interesting tools for the delineation or genotyping of bacterial species. We concluded that a combination of Box-, Eric- and Miniprimer-PCR results is a rapid and reliable alternative for discrimination among P. fluorescens isolates, contributing to epidemiological studies.
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