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Articles by Peng Zhao
Total Records ( 7 ) for Peng Zhao
  Peng Yu , Shumin Sun , Guanglei Li , Yong Zhang , Yan Cheng , Peng Zhao , Qingbo Mao and Shuai Zhao
  The energy storage technique is one of the most effective means for the real-time wind power regulation to enhance the stability of wind power. Firstly, this study discussed the operation mode of energy storage system in the course of real-time wind power balance. Also, it was proposed that the energy storage system applied into the real-time wind power regulation should be of the properties of high power density, high energy density and long cycle life. From the standpoint of practical engineering, this study carried out a comparative analysis on the energy storage properties of different energy storage elements. Furthermore, the research status and engineering development of each energy storage element were introduced. In view of the deficiency of single energy storage pattern, the composition of hybrid energy storage system was described. According to the operation requirement for balancing the fluctuant wind power in real-time, a comparative research on the topology of battery-supercapacitor energy storage system was carried out. This study hopes to supply new hints for enhancing the power controllability of wind farm.
  Peng Zhao , Zhizhong Cui , Chengtai Ma , Xuan Dong and Deqing Li
  To investigate the Avain leukosis virus infection status in China native chicken flocks, 2530 serum samples from 26 kinds of China native chickens were collected and detected using the Avian Leukosis Virus Antibody Test kit (ALV-A/B) and Avian Leukosis Virus Antibody Test kit-Subgroup J (ALV-J). The results showed that among 2530 sera samples 118 samples were positive for ALV-A/B, 332 samples were positive for ALV-J and 35 samples were positive for both ALV-A/B and ALV-J. The positive rate for ALV-A/B, ALV-J and their co-infection was 0-16.67, 0-59.60 and 0-5.05%, respectively. This investigation suggests that the infection of ALV-A/B, ALV-J and their co-infection do exist or even very popular in some native chickens flocks in China and the prevention of ALV should be given more attention.
  Peng Zhao , Cheng-Tai Ma and Zhi-Zhong Cui
  To study the quasispecies diversity and mutations of highly pathogenic porcine reproductive and respiratory syndrome virus emerged in 2006 in China, the ORF5 gene of a wild HP-PRRSV strain SD0612 was amplified and the amplified product was cloned into T vector, all positive clones were sequenced and analyzed with DNA Star software. To exclude sequencing errors, another very conservative gene ORF6 with the similar product length to ORF5 gene was amplified, cloned and sequenced as above. About 60 positive clones of ORF5 gene were obtained, sequenced and analyzed and their nucleic acid sequence homology ranging from 97.7-100%, a total of 78 base mutants between them were found. Among these 60 sequence 17 sequence was quite the same and be determined as the sequence of advantage quasispecies which account for the magerority of SD0612. For the conservative ORF6 gene, 54 positive clones were obtained and their nucleic acid sequence homology ranging from 99.2-100%, among these 54 sequences 46 sequences was quite the same, a total of 8 base mutants were found between them. The GP5 sequence for advantages quasispecies of SD0612 was also compared with other reference GP5 sequence of 20 highly pathogenic PRRSV mutants and they shared only 93.5-98.5% homology, total 23 mutation points between them was found and some mutations located in the epitopes. This study indicate the quasispecies diversity of SD0612 can be indicated by the sequence diversity of ORF5 gene, the mutations between different HP-PRRSV mutants is also great although they have the same deletions in NSP2 gene.
  Hao Chen , Yi-Xin Wang , Peng Zhao , Jian-Liang Li and Zhi-Zhong Cui
  To explore the reason of acute fibrosarcoma formation in chickens, researchers detected the existence of viral antigens in the cytoplasm of infected CEFs by monoclonal antibody against ALV-J and rabbit polyclonal antibody against oncogene-fps protein. PCR amplified the whole genome sequence of the replication-competent ALV-J (SD1005), alignment of the complete sequence demonstrated that it has no remarkable mutation in the genome and appears not to be the real pathogen inducing the acute tumorigensis. Additionally, we obtained several genome sequences associated with ALV-J (Fu-J1-6) which is defective with parts of gag, pol and env genes replaced by a sequence of fps gene. The sequences of Fu-Js revealed that their genomes are closely related to that of SD1005 and probably derived from it in accordance with the high homology (>99%) in env gene between them. Notably, compared with homologous region of Fujinami and PRCII, the obtained v-fps genes in the study have two deletion mutations at both ends with the major diversity at the 3'-fps end, especially. So, researchers infer that it seems to be unstable for junction site in the process of recombination between c-fps oncogene and retrovirus ALV, a majority of variable individuals at gene level (quasispecies) will generate, one strain of which has biological activity at least. Obviously, the induction of acute fibrosarcomas in the experiment occurs through mechanisms involving the direct activation of viral oncogene v-fps in Fu-Js rather than insertional activation of cellular oncogene c-fps, revealing that the tumorigenesis induced by ALV-J is probable to involve fps oncogene besides for the myc oncogene. This is the first report on acute fibrosarcomas caused by ALV-J containing v-fps oncogene since the identification of this virus in 1988.
  Chengtai Ma , Yaoyao Zhang , Zhizhong Cui , Xin Wang and Peng Zhao
  To investigate the co-infection status of Reticuloendotheliosis Virus (REV) with Chicken Anemia Virus (CAV) in chicken flocks of live poultry market, 370 spleen samples from six 817 broiler chicken flocks were collected and it’s DNA was detected by nucleic acid hybridization with the REV-specific and CAV-specific probes. Out of 370, 20 samples could be detected by REV probe and 87 samples detected by CAV probe. The positive rate of co-infection of REV with CAV was 3.2%. These results indicated that co-infection of REV and CAV also existed in healthy broiler chicken flocks in the live poultry market. A subclinical infection of REV and CAV might be an important channel for cross-infection in different chicken flocks.
  Shuai Su , Ning Cui , Yixin Wang , Aijun Sun , Pengfei Qi , Zimeng Chen , Peng Zhao and Zhizhong Cui
  Marek’s Disease Virus (MDV) Chinese strain GX0101 was the first reported recombinant MDV field strain with one Reticuloendotheliosis Virus (REV) Long Terminal Repeat (LTR) insert. REV LTR in the GX0101 genome increased the potential for horizontal transmission. REV LTR fragment has the biggest impact on SORF2 gene. Researchers detected the influence of SORF2 gene deletion on its horizontal transmission ability by real time PCR. The result shows that GX0101ΔSorf2 possesses similar replication ability with bac-GX0101 no mater on the level of lymphocyte or feather follicle. Although, researchers can detect MDV positive in feather follicles of sentinel in contact chickens at age 10 in both groups respectively, the number of SPF chickens infected with bac-GX0101 by contact is higher than the number of GX0101ΔSorf2 infection group. Therefore, SORF2 gene has certain effect on the horizontal transmission ability of the virus.
  Weihua Li , Yang Li , Bohua Zhang , Xuan Dong , Zhizhong Cui and Peng Zhao
  To investigate the Reticuloendotheliosis virus infection status in large-scale breeder chicken farms of China, 563 serum samples from 4 great-grandparents breeder farms, 6557 serum samples from 8 grandparents breeder farms, 312 serum samples from parents breeder farms, totally 7432 serum samples were collected and examined using the Reticuloendotheliosis Virus Antibody Test kit. The results showed that REV-antibody positive rates of great-grandparents breeder flocks, grandparent breeder flocks and parents breeder flocks were 0-31, 0-47 and 4.23-42.50%, respectively. REV-antibody positive rate has close relationship with incidence of REV infection history and tumor complaints. This study suggests that REV infection is very common in China large-scale breeder chicken farms and the prevention of REV should be given more attention this is the largest scale serological survey for breeder chickens in China so far.
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