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Articles by Pedram Kashiani
Total Records ( 3 ) for Pedram Kashiani
  Pedram Kashiani and Ghizan Saleh
  Problem statement: Genetic correlations among traits refer to the extent of relatedness among them due to">genetic causes. Estimating genetic correlations for quantitative traits is tedious if done manually. Approach: However, the use of the computer software SAS, applying mixed-model analysis of variance has facilitated many recent studies in evolutionary quantitative genetics. Results: In this two-way statistical model, the variance component corresponding to the random statement is the covariance associated with a level of the random factor across levels of the fix factor. Therefore, the SAS model has a natural application for estimating genetic correlations among traits measured. Correlation studies were undertaken for 10 yield-related traits on a series of near-homozygous sweet corn inbred lines obtained from various tropical source populations. The SAS program was used to estimate genetic correlation coefficients among traits observed, where effects of blocks were considered fixed while effects of inbred lines as random. The "asycov" was added to the "PROC MIXED" statement in order to produce the variance-covariance matrix of variance components. The "type = UN" option requested in "RANDOM" statement resulted in an unstructured covariance matrix for each inbred line being estimated, while the "G" and "GCORR" options produced genetic variance-covariance matrix and genetic correlation matrix between traits, respectively. Results showed that there was no significant difference between genetic correlations estimated by SAS MIXED model and those estimated by manual calculation. Conclusion/Recommendations: This indicated that SAS has the natural capability to estimate genetic correlations among traits measured, as opposed to manual methods employing quantitative genetics equations.
  Ahmed Mahmood Ibrahim , Fatimah Binti Kayat , Dwi Susanto , Mohammed Ariffullah and Pedram Kashiani
  Kenaf (Hibiscus cannabinus L.) is an important crop cultivated for fiber production. The haploid production using tissue culture technique is a fast and efficient tool for developing new varieties in comparatively short-time. Kenaf HF992 cultivar was chosen as an explants material, several trials were carried out to investigate the gynogenesis ability before we succeed to get high percentage of callus induction from ovule. Flower buds at the appropriate stage of ovule development were sterilized and the ovules were carefully excised from the flowers and underwent to various pretreatments and inoculated onto MS media supplemented with different combinations of Plant Growth Regulators (PGR) like NAA (a-naphthaleneacetic acid), BAP (N6-benzyladenine), 2iP (N6-(2-isopentenyl) adenine) and TDZ (Thidiazuron) and kept in the dark place for different periods before transferred to light conditions. The high frequency of callus induction (98%) was observed when the ovules collected from the flower bud size of 22-24 mm length after 2-4 weeks of the flower initiation and inculcated onto the optimized semi solid MS medium (3:1 gelrite:agar by weight) fortified with 3.0 mg L‾1 NAA+3.0 mg L‾1 2iP.
  Ahmed Mahmood Ibrahim , Fatimah Binti Kayat , Dwi Susanto , Pedram Kashiani and Mohammed Arifullah
  Regeneration into haploid plantlets had been obtained in spring onion using flower and ovary culture. Flowers and ovaries were cultured into media using two protocols (A and B) and the ability to produce callus or somatic embryogenesis were invistigated. Flowers around 3.0-5.0 mm long were collected, whole flower or ovary which were excised from flowers using dissecting microscope were cultured into BDS medium supplemented 2.0 mg L–1 2, 4-D and 2.0 mg L–1 BAP fortified with 100 g L–1 sucrose, 200 mg L–1 proline, 500 mg L–1 myo-inositol (protocol A) or into BDS media supplemented 2.0 mg L–1 2,4-0 and 2.0 mg L–1 BAP for 14 days, then sub-cultured by regeneration medium (BDS) supplemented with 1.0 mg L–1 NAA and 2.0 mg L–1 2iP (protocol B). Embryos were emerged from ovary wall after around 4-5 months, high frequency of embryogenesis induction was produced from ovaries that were using protocol A. While the less percentage observed from flower culture using protocol B.
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