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Articles by Pawitra Pulbutr
Total Records ( 6 ) for Pawitra Pulbutr
  Pawitra Pulbutr , Klangnapa Thunchomnang , Karakate Lawa , Apiwat Mangkhalathon and Pattarapong Saenubol
  Zingerone is a non-volatile pungent compound mostly found in ginger which is a herbal medicine used for various purposes. The anti-obesity actions of ginger and zingerone have also been documented. This study was aimed to investigate the effects of zingerone on adipocyte lipolysis in adipocytes isolated from Normal Pellet Diet (NPD)-fed rats and High Fat Diet (HFD)-fed rats. Adipocyte suspensions were prepared from the epididymal fat pads of male Wistar rats by using the collagenase digestion method. The concentrations of Free Fatty Acid (FFA) in the incubation media were used as an index of adipocyte lipolysis. Zingerone at the concentration of 1,000 μM significantly increased both basal lipolysis and isoprenaline (0.l μM)-induced lipolysis in adipocytes derived from the NPD-fed rats with the FFA concentrations of 682.92±37.00 μM mL-1 Packed Cell Volume (PCV)/ h and 1,361.51±62.90 μM mL-1 PCV/ h, respectively (p<0.05; n = 4). In adipocytes derived from the HFD-fed rats, zingerone at any concentration tested had no effect on the basal lipolysis. Zingerone only at the concentration of 100 μM significantly stimulated the isoprenaline-induced lipolysis with the FFA concentration of 1,383.88±36.73 μM mL-1 PCV/ h (p<0.05; n = 4) in adipocytes derived from the HFD-fed rats. In conclusion, zingerone appeared to produce less lipolytic actions in adipocytes derived from the HFD-fed rats. The reason for the lower lipolytic response to zingerone in HFD-derived adipocytes should be investigated further, especially in the molecular aspects.
  Pawitra Pulbutr , Atika Jaruchotikamol , Jirawan Chaiyachart , Pannee Sonrasee and Wannaporn Poungpan
  (-)-Epigallocatechin-3-gallate (EGCG), the major catechins found in green tea, has been known to possess various pharmacological activities. It has been demonstrated that EGCG affects on several adipocyte functions, involving in its weight-controlling action. This study was aimed to investigate the effects of EGCG on adipocyte lipolysis in adipocytes derived from Normal Pellet Diet (NPD)-fed rats and High Fat Diet (HFD)-fed rats. Sixteen male Wistar rats were divided into 2 groups (8 rats per group). The rats in each group were fed with NPD or HFD for 3 weeks. The adipocyte suspensions were prepared by the collagenase digestion method. The concentration of Free Fatty Acid (FFA) was measured after 1 h-incubation as an index of adipocyte lipolysis. EGCG at every concentration tested (1, 5 and 10 μM) significantly stimulated basal lipolysis in adipocytes derived from the NPD-fed rats with the FFA concentrations of 453.67±25.57, 458.87±26.84 and 443.97±19.75 μM mL-1 Packed Cell Volume (PCV)/h, respectively (p<0.05). In adipocytes derived from the HFD-fed rat, EGCG only at the concentration of 5 μM significantly increased basal adipocyte lipolysis with the FFA concentration of 1,091.75±220.56 μM mL-1 PCV h-1 (p<0.05; n = 4). Conversely, EGCG at any of the concentrations tested did not cause a significant change in isoprenaline (0.1 μM)-induced lipolysis in both adipocyte groups. These results indicate a lower lipolytic action of EGCG in adipocytes derived from the HFD-fed rats. Further experiments thus should be done to explore the reason for the difference in the lipolytic response to EGCG between these adipocytes.
  Pawitra Pulbutr , Wanida Caichompoo , Pilanthana Lertsatitthanakorn , Methin Phadungkit and Sakulrat Rattanakiat
  The essential oil from the fruits of Amomum uliginosum K.D. Koenig has been commonly used in Thai herbal formula for the treatment of gastrointestinal diseases. However, the biological activity of the essential oil obtained from A. uliginosum fruits has never been reported. This study was aimed to investigate the pharmacological activities and cytotoxic effect of the essential oil derived from the fruit of A. uliginosum. The essential oil derived from the authentic fruits and the commercial fruits were prepared by distillation with the yield of 2.90±0.00 and 2.90±0.30% v/w, respectively. The chemical constituents of the essential oil were analyzed by GC-MS. Twenty one and 13 chemical compounds were found in the essential oil derived from the authentic and commercial fruits, respectively. The major chemical compound presented in the essential oil from both sources was bornyl acetate. The essential oil exhibited an antibacterial activity against gastrointestinal disorder-related bacteria with the lowest MIC of 75 μL mL-1 against E. coli O157:H7 DMST 12743 and S. aureus DMST 8013. The essential oil exerted the cytotoxic effect on 3T3 fibroblasts with the IC50 of 0.0725±0.0045 μL mL-1. The strong antimutagenic effect in Salmonella typhimurium TA 98 was found at the concentration of 3.75 mL plate-1. These results scientifically confirmed the use of the essential oil derived from A. uliginosum for the medicinal purposes. Additionally, its potential application for the use as cancer chemoprevention is also indicated from this study.
  Pawitra Pulbutr , Natcha Saweeram , Thitiworada Ittisan , Hattaya Intrama , Achida Jaruchotikamol and Benjamart Cushnie
  Background: Coccinia grandis (ivy gourd) an edible plant widely consumed in many Southeast Asian countries has been used traditionally for the treatment of diabetes mellitus. However, its role in the regulation of carbohydrate-digesting enzymes, especially α-amylase and α-glucosidase has not been clearly established. Materials and Methods: The aqueous leaf and stem extracts of Coccinia grandis (the CGL and the CGS extracts, respectively) were prepared and their actions toward in vitro activities of α-amylase and α-glucosidase were examined. The activities of α-amylase and α-glucosidase were indicated by the amounts of maltose and p-nitrophenol generated in the reactions, respectively. Results: The CGL and the CGS extracts possessed an α-amylase inhibitory action with the IC50s of 8.09±0.72 and 8.06±1.27 mg mL–1, respectively. The CGL extract produced a mixed inhibition against α-amylase, whilst the CGS extract inhibited the enzyme in an uncompetitive manner. The CGL and the CGS extracts exhibited an inhibitory action against α-glucosidase enzyme with the IC50s of 77.66±9.16 μg mL–1 and 0.75±0.11 mg mL–1, respectively. Both extracts acted as mixed enzyme inhibitors against α-glucosidase. Conclusion: These results support the traditional use of Coccinia grandis in diabetic patients, especially for the control of postprandial plasma glucose level. However, further studies on identification of the active phytochemicals acting as enzyme inhibitors and in vivo carbohydrate-digesting enzyme inhibition are required to verify its potential clinical use in diabetes mellitus.
  Pawitra Pulbutr , Sakulrat Rattanakiat , Wanwisa Khunawattanakul , Kritsanee Saramunee and Bunleu Sungthong
  Background and Objective: Streptococcus mutans is a major causative pathogen of dental caries, which is a multifactorial oral infection resulting in progressive tooth destruction. Several medicinal plants, including Morus alba (mulberry tree) have been used in folk medicine for the treatment and prevention of dental caries. The objectives of this study were to evaluate the antibacterial and anti-cariogenic activities of the M. alba leaf extracts against S. mutans and to develop a chewing gum containing the extract. Materials and Methods: The MIC and MBC were determined by using broth dilution and drop plate methods, respectively. Biofilm formation and glycolytic pH drop assays were performed to evaluate the anti-cariogenic activity. High-performance liquid chromatography was used for phytochemical analysis of the extracts. The chewing gum containing the M. alba leaf ethanolic extract was prepared by varying different types and concentrations of ingredients. The freeze-thaw cycle testing was conducted to determine the stability of the developed chewing gum. Results: The ethanolic and aqueous M. alba leaf extracts possessed antibacterial activity against S. mutans with the MICs of 0.14 and 5 mg mL–1, respectively. The ethanolic extract at the sub-MICs significantly inhibited both acid production and biofilm formation of S. mutans. The phytochemical analysis showed that the concentrations of chlorogenic acid and rutin in the ethanolic extract were significantly higher than those in the aqueous extract. Two satisfactory chewing gum formulas with good stability, gum A and B, were obtained. The gum B formula was found to have the better performance. Conclusion: The results from this study demonstrated the anti-cariogenic role of M. alba leaves even at the low concentration of sub-MICs. The prepared chewing gum B formula can potentially be further developed as a medicated chewing gum for the prevention of dental caries in the near future.
  Pawitra Pulbutr , Sakulrat Rattanakiat , Natnicha Phetsaardeiam , Patcharapa Modtaku , Rattazart Denchai , Achida Jaruchotikamol and Wanwisa Khunawattanakul
  Background and Objective: Streptococcus mutans is a dominant causative pathogen of dental caries, which is a major oral health problem affecting million people worldwide. Derris reticulata is a medicinal plant possessing antimicrobial activity against several Gram-positive pathogenic bacteria. None the less, its effects on growth and cariogenic properties of S. mutans has not been clearly established. This study aimed to investigate the antibacterial and anti cariogenic activities of the D. reticulata ethanolic stem extract. Materials and Methods: The TLC analysis was performed to authenticate the D. reticulata sample. Minimum inhibition concentration and minimum bactericidal concentration were determined by using broth dilution and drop plate methods, respectively. Sucrose dependent and sucrose independent-adherences, biofilm formation and glycolytic pH drop assays were performed to evaluate the anticariogenic activity. Results: The ethanolic stem extract of D. reticulata possessed the antibacterial activity against S. mutans with the MIC and MBC of 0.875±0.250 and 1.750±0.500 mg mL–1, respectively. The extract at the lower concentrations of sub-MIC also had significant inhibitory actions against the cariogenic properties of S. mutans, including surface adherence, biofilm formation and glycolytic acid production. Conclusion: The D. reticulata stem extract had a substantial anticariogenic activities and thus potentially be developed as an oral health care product for dental caries prevention in the near future.
 
 
 
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